Elazığ’da Yetişen Polygonum cognatum Meissn (Madımak) Bitki Ekstraktlarının In vitro Biyolojik Aktiviteleri ve Bazı Fitokimyasal Bileşenlerinin Belirlenmesi
Abstract
Halk arasında bitkilerin çeşitli kısımları kullanım amacına yönelik basit işlemlerden geçirilerek gerek gıda, gerekse tedavi amacıyla yaygın olarak kullanılmaktadır. Bitkiler, insan beslenmesinde çok önemli bir yer tutar ve insan sağlığı açısından bazı önemli bileşenler içermektedir. Dünyadaki bitkisel çeşitliliğin fazlalığı, çoğu bitkinin biyolojik etkileri ve etki mekanizmaları hakkındaki bilimsel verilerin hala yetersiz olması bu konuya olan ilgiyi her geçen gün artırmaktadır. Bu çalışmanın amacı, Elazığ yöresinden toplanan ve özellikle gıda olarak mevsiminde bolca tüketilen Polygonum cognatum Meissn (Madımak, Kuşekmeği) bitkisinin etanol, metanol, aseton ve hekzan ekstraktlarının antiradikal aktiviteleri, fitokimyasal bileşenleri, antimikrobiyal ve in vitro antikanserojen aktivitelerinin araştırılmasıdır. Çalışma sonuçlarımıza göre, P. cognatum Meissn özütlerinin ABTS radikal giderme aktivitelerinin DPPH a kıyasla daha iyi sonuç verdiği görüldü. Ayrıca ABTS radikal giderme aktivitelerinin standart bir antioksidan olan askorbik asite (Vitamin C) yakın değerlerde olduğu bulundu. Polygonum cognatum Meissn’ in yüksek miktarda fitosteroller, K ve D vitaminleri, α-tokoferol, flavonoidler, proantosiyanidin, fenolikler ve doymamış yağ asitleri içerdiği tespit edilmiştir. Bitki ekstraktlarının antimikrobiyal etkileri Escherichia coli, Bacillus subtilis, Klebsiella pneumoniae, Staphylococcus aureus and Candida albicans mikroorganizmaları kullanılarak Disk Difüzyon ve Minimum İnhibitör Konsantrasyonu (MİK) yöntemleri ile belirlendi. Bütün ekstraktların tüm mikroorganizmalara karşı antimikrobiyal aktivite gösterdiği görüldü. Aynı zamanda Polygonum cognatum Meissn ekstraktlarının, MCF-7, PC3 ve MKN-45 kanser hücre hatları üzerinde kontrole göre özellikle yüksek konsantrasyonlarda oldukça iyi sitotoksik etkiler sergilediği görüldü. Kanser hücreleri içerisinde en yüksek etkiyi ise MKN-45 (insan mide kanseri) hücre hattında göstermiştir. Elde edilen sonuçlar antioksidan ve antipiroliferatif etki gösteren ve biyoaktif bileşikleri içeren Polygonum cognatum Meissn bitkisinin insanlar tarafından tüketilmeye devam edilebileceğini göstermiştir.
Supporting Institution
Bingöl Üniversitesi
Project Number
FEF.2018.00.002
Thanks
Yazarlar; Prof. Dr. Ökkeş YILMAZ’a, Prof. Dr. Sevda KIRBAĞ’a, Doç. Dr. Serhat KESER’ e, Doç Dr. Sevgi Durna DAŞTAN’a, Dr. Mustafa Ersin PEKDEMİR’ e ve Şule İNCİ’ ye katkılarından dolayı teşekkür eder. Yazarlar ayrıca, bu çalışmanın gerçekleşmesine finansal destek sağlayan FEF.2018.00.002 nolu BÜBAP projesine teşekkür eder.
References
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- Baytop, T. (1999). Türkiye'de bitkiler ile tedavi: geçmişte ve bugün: Nobel Tıp Kitabevleri.
- BİLGİN, A., & KOCABAĞLI, N. (2010). Etlik piliç beslemede esansiyel yağların kullanımı. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, 36(1), 75-82.
- Boivin, D., Blanchette, M., Barrette, S., Moghrabi, A., & Beliveau, R. (2007). Inhibition of cancer cell proliferation and suppression of TNF-induced activation of NFκB by edible berry juice. Anticancer research, 27(2), 937-948.
- Christie, W. W. (1989). Gas chromatography and lipids: Oily.
- Collins, C., & Lyne, P. (1987). Microbiological Methods Butter Morths & Co (Publishers) Ltd. London. 450pp.
- Cragg, G. M., & Newman, D. J. (2005). Plants as a source of anti-cancer agents. Journal of ethnopharmacology, 100(1-2), 72-79.
- Endo, Y., Usuki, R., & Kaneda, T. (1985). Antioxidant effects of chlorophyll and pheophytin on the autoxidation of oils in the dark. II. The mechanism of antioxidative action of chlorophyll. Journal of the American Oil Chemists' Society, 62(9), 1387-1390.
- Eruygur, N., Ucar, E., Ataş, M., Ergul, M., Ergul, M., & Sozmen, F. (2020). Determination of biological activity of Tragopogon porrifolius and Polygonum cognatum consumed intensively by people in Sivas. Toxicology Reports, 7, 59-66.
- FAYDAOĞLU, E., & SÜRÜCÜOĞLU, M. (2013). TIBBİ VE AROMATİK BİTKİLERİN ANTİMİKROBİYAL, ANTİOKSİDAN AKTİVİTELERİ VE KULLANIM OLANAKLARI. Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 6(2), 233-265.
- Fiedorov, A. (1985). Rastitelnie Resursi SSCB. Nauka, Liningrad, 260, 61-97.
- Hartwell, J. L. (1982). Plants used against cancer: a survey: Quarterman Publications.
- Ho, C.-T., Ferraro, T., Chen, Q., Rosen, R. T., & Huang, M.-T. (1994). Phytochemicals in teas and rosemary and their cancer-preventive properties: ACS Publications.
- Hsieh, T.-c., Wu, P., Park, S., & Wu, J. M. (2006). Induction of cell cycle changes and modulation of apoptogenic/anti-apoptotic and extracellular signaling regulatory protein expression by water extracts of I'm-Yunity™(PSP). BMC complementary and alternative medicine, 6(1), 30.
- İnci, Ş., Dalkılıç, L. K., Dalkılıç, S., & Kırbağ, S. (2019). Helvella leucomelaena (Pers.) Nannf.’ın antimikrobiyal ve antioksidan Etkisi. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, 20(2), 249-253.
- İnci, Ş., & Kırbağ, S. (2018). Terfezia claveryi Chatin’in besinsel içeriği, antioksidan ve antimikrobiyal aktivitesi. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, 19(2), 138-143.
- Jain, A., Katewa, S., Galav, P., & Nag, A. (2007). Unrecorded ethnomedicinal uses of biodiversity from Tadgarh-Raoli Wildlife sanctuary, Rajasthan, India. 雲南植物研究, 29(3), 337-344.
- Keser, S., Celik, S., & Turkoglu, S. (2013). Total phenolic contents and free-radical scavenging activities of grape (Vitis vinifera L.) and grape products. International journal of food sciences and nutrition, 64(2), 210-216.
- Keser, S., Kaygili, O., Keser, F., Tekin, S., Yilmaz, Ö., Demir, E., . . . Sandal, S. (2018). Phytochemical Composition, Antiradical, Antiproliferative and Antimicrobial Activities of Capsicum frutescens L. Analytical Chemistry Letters, 8(5), 642-652.
- Kim, D.-O., Chun, O. K., Kim, Y. J., Moon, H.-Y., & Lee, C. Y. (2003). Quantification of polyphenolics and their antioxidant capacity in fresh plums. Journal of agricultural and food chemistry, 51(22), 6509-6515.
- KÜÇÜKBAY, H., Mumcu, A., Tekin, S., & SANDAL, S. (2016). Synthesis and evaluation of novel $ N, N'$-disubstituted benzimidazolium bromides salts as antitumor agents. Turkish Journal of Chemistry, 40(3), 393-401.
- Morales, G., Paredes, A., Sierra, P., & Loyola, L. A. (2008). Antimicrobial activity of three Baccharis species used in the traditional medicine of Northern Chile. Molecules, 13(4), 790-794.
- Nishina, A., Kubota, K., Kameoka, H., & Osawa, T. (1991). Antioxidizing component, musizin, inrumex japonicus houtt. Journal of the American Oil Chemists Society, 68(10), 735-739.
- Onen, H., Yılar, M., & Kaya, C. (2009). Phenoleic composition of madimak (Polygonum cognatum Meissn.) plants. Paper presented at the 3rd Plant protection Congress. Abstract Book.
- Önen, H., Altuntaş, E., ÖzGöz, E., Bayram, M., & Özcan, S. (2014). Moisture effect on physical properties of knotweed (Polygonum cognatum Meissn.) seeds.
- Sanchez-Machado, D., Lopez-Hernandez, J., & Paseiro-Losada, P. (2002). High-performance liquid chromatographic determination of α-tocopherol in macroalgae. Journal of Chromatography A, 976(1-2), 277-284.
- SARAÇ, H., DAŞTAN, T., DEMİRBAŞ, A., DAŞTAN, S. D., KARAKÖY, T., & DURUKAN, H. Madımak (Polygonum cognatum Meissn.) Bitki Özütlerinin Besin Elementleri ve In Vitro Antikanserojen Aktiviteleri Yönünden Değerlendirilmesi. Ziraat Fakültesi Dergisi, 340-347.
- Shimada, K., Fujikawa, K., Yahara, K., & Nakamura, T. (1992). Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. Journal of agricultural and food chemistry, 40(6), 945-948.
- Shu, Y.-Z. (1998). Recent natural products based drug development: a pharmaceutical industry perspective. Journal of natural products, 61(8), 1053-1071.
- Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. M. (1999). [14] Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent Methods in enzymology (Vol. 299, pp. 152-178): Elsevier.
- Tatli, A. (1988). Important Range Plants of Erzurum Province. Food and Agriculture Organisation of the United Nations, Rome, 43.
- TOKUR, O., & AKSOY, A. (2017). In vitro sitotoksisite testleri. Harran Üniversitesi Veteriner Fakültesi Dergisi, 6(1), 112-118.
- Wu, L.-c., Chang, L.-H., Chen, S.-H., Fan, N.-c., & Ho, J.-a. A. (2009). Antioxidant activity and melanogenesis inhibitory effect of the acetonic extract of Osmanthus fragrans: A potential natural and functional food flavor additive. LWT-Food Science and Technology, 42(9), 1513-1519.
- Yalçın, A. S., Yılmaz, A. M., AltunDAğ, E. M., & KOçtürK, S. (2017). Kurkumin, kuersetin ve çay kateşinlerinin anti-kanser etkileri.
- Yıldırım, A., Mavi, A., & Kara, A. A. (2003). Antioxidant and antimicrobial activities of Polygonum cognatum Meissn extracts. Journal of the Science of Food and Agriculture, 83(1), 64-69.
Determination of In Vitro Biological Activities and Some Phytochemical Components of Polygonum cognatum Meissn (Madımak) Plant Grown in Elazığ
Abstract
Among the people, various parts of the plants are passed through simple processes for their intended use and are widely used for both food and therapeutic purposes. Plants have a very important place in human nutrition and contain some important components in terms of human health. Excess of the global herbal diversity, the lack of the scientific data on the biologic effects and mechanism of action of most plants are increasing the interest in this issue day by day. The aim of this study, is to investigate the antiradical activities, phytochemical components, antimicrobial and in vitro anticancer activities of the ethanol, methanol, acetone and hexane extracts of the Polygonum cognatum Meissn (Madımak, Kuşekmeği) collected from Elazığ and consumed abundantly as a food in season. According to our study results, ABTS radical scavenging activities of Polygonum cognatum Meissn extracts were found to give better results compared to DPPH. Also, ABTS radical scavenging activities were found to be close to ascorbic acid, a standard antioxidant. It was established that Polygonum cognatum Meissn contains high amounts of phytosterols, vitamin K and D, α-tocopherol, flavonoids, proanthocyanidin, phenolics and unsaturated fatty acids. Antimicrobial effects of Polygonum cognatum Meissn extracts were determined by Disc Diffusion and the Minimum Inhibitory Concentration (MIC) methods using Escherichia coli, Bacillus subtilis, Klebsiella pneumoniae, Staphylococcus aureus and Candida albicans microorganisms. It was observed that all plant extracts showed antimicrobial activity against all microorganisms. However, Polygonum cognatum Meissn extracts were exhibited good cytotoxic effects on MCF-7, PC3 and MKN-45 cancer cell lines than control, especially at higher concentration. Among the cancer cells, MKN-45 (human stomach cancer) showed the highest effect in the cell lines. Consequently, obtained results indicate that Polygonum cognatum Meissn may continue to be consumed by humans owing to it has potentially antioxidant, antiproliferative properties and including bioactive compounds.
Project Number
FEF.2018.00.002
References
- Amaeze, O., Ayoola, G., Sofidiya, M., Adepoju-Bello, A., Adegoke, A., & Coker, H. (2011). Evaluation of antioxidant activity of Tetracarpidium conophorum (Müll. Arg) Hutch & Dalziel leaves. Oxidative medicine and cellular longevity, 2011.
- Baytop, T. (1999). Türkiye'de bitkiler ile tedavi: geçmişte ve bugün: Nobel Tıp Kitabevleri.
- BİLGİN, A., & KOCABAĞLI, N. (2010). Etlik piliç beslemede esansiyel yağların kullanımı. İstanbul Üniversitesi Veteriner Fakültesi Dergisi, 36(1), 75-82.
- Boivin, D., Blanchette, M., Barrette, S., Moghrabi, A., & Beliveau, R. (2007). Inhibition of cancer cell proliferation and suppression of TNF-induced activation of NFκB by edible berry juice. Anticancer research, 27(2), 937-948.
- Christie, W. W. (1989). Gas chromatography and lipids: Oily.
- Collins, C., & Lyne, P. (1987). Microbiological Methods Butter Morths & Co (Publishers) Ltd. London. 450pp.
- Cragg, G. M., & Newman, D. J. (2005). Plants as a source of anti-cancer agents. Journal of ethnopharmacology, 100(1-2), 72-79.
- Endo, Y., Usuki, R., & Kaneda, T. (1985). Antioxidant effects of chlorophyll and pheophytin on the autoxidation of oils in the dark. II. The mechanism of antioxidative action of chlorophyll. Journal of the American Oil Chemists' Society, 62(9), 1387-1390.
- Eruygur, N., Ucar, E., Ataş, M., Ergul, M., Ergul, M., & Sozmen, F. (2020). Determination of biological activity of Tragopogon porrifolius and Polygonum cognatum consumed intensively by people in Sivas. Toxicology Reports, 7, 59-66.
- FAYDAOĞLU, E., & SÜRÜCÜOĞLU, M. (2013). TIBBİ VE AROMATİK BİTKİLERİN ANTİMİKROBİYAL, ANTİOKSİDAN AKTİVİTELERİ VE KULLANIM OLANAKLARI. Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 6(2), 233-265.
- Fiedorov, A. (1985). Rastitelnie Resursi SSCB. Nauka, Liningrad, 260, 61-97.
- Hartwell, J. L. (1982). Plants used against cancer: a survey: Quarterman Publications.
- Ho, C.-T., Ferraro, T., Chen, Q., Rosen, R. T., & Huang, M.-T. (1994). Phytochemicals in teas and rosemary and their cancer-preventive properties: ACS Publications.
- Hsieh, T.-c., Wu, P., Park, S., & Wu, J. M. (2006). Induction of cell cycle changes and modulation of apoptogenic/anti-apoptotic and extracellular signaling regulatory protein expression by water extracts of I'm-Yunity™(PSP). BMC complementary and alternative medicine, 6(1), 30.
- İnci, Ş., Dalkılıç, L. K., Dalkılıç, S., & Kırbağ, S. (2019). Helvella leucomelaena (Pers.) Nannf.’ın antimikrobiyal ve antioksidan Etkisi. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, 20(2), 249-253.
- İnci, Ş., & Kırbağ, S. (2018). Terfezia claveryi Chatin’in besinsel içeriği, antioksidan ve antimikrobiyal aktivitesi. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, 19(2), 138-143.
- Jain, A., Katewa, S., Galav, P., & Nag, A. (2007). Unrecorded ethnomedicinal uses of biodiversity from Tadgarh-Raoli Wildlife sanctuary, Rajasthan, India. 雲南植物研究, 29(3), 337-344.
- Keser, S., Celik, S., & Turkoglu, S. (2013). Total phenolic contents and free-radical scavenging activities of grape (Vitis vinifera L.) and grape products. International journal of food sciences and nutrition, 64(2), 210-216.
- Keser, S., Kaygili, O., Keser, F., Tekin, S., Yilmaz, Ö., Demir, E., . . . Sandal, S. (2018). Phytochemical Composition, Antiradical, Antiproliferative and Antimicrobial Activities of Capsicum frutescens L. Analytical Chemistry Letters, 8(5), 642-652.
- Kim, D.-O., Chun, O. K., Kim, Y. J., Moon, H.-Y., & Lee, C. Y. (2003). Quantification of polyphenolics and their antioxidant capacity in fresh plums. Journal of agricultural and food chemistry, 51(22), 6509-6515.
- KÜÇÜKBAY, H., Mumcu, A., Tekin, S., & SANDAL, S. (2016). Synthesis and evaluation of novel $ N, N'$-disubstituted benzimidazolium bromides salts as antitumor agents. Turkish Journal of Chemistry, 40(3), 393-401.
- Morales, G., Paredes, A., Sierra, P., & Loyola, L. A. (2008). Antimicrobial activity of three Baccharis species used in the traditional medicine of Northern Chile. Molecules, 13(4), 790-794.
- Nishina, A., Kubota, K., Kameoka, H., & Osawa, T. (1991). Antioxidizing component, musizin, inrumex japonicus houtt. Journal of the American Oil Chemists Society, 68(10), 735-739.
- Onen, H., Yılar, M., & Kaya, C. (2009). Phenoleic composition of madimak (Polygonum cognatum Meissn.) plants. Paper presented at the 3rd Plant protection Congress. Abstract Book.
- Önen, H., Altuntaş, E., ÖzGöz, E., Bayram, M., & Özcan, S. (2014). Moisture effect on physical properties of knotweed (Polygonum cognatum Meissn.) seeds.
- Sanchez-Machado, D., Lopez-Hernandez, J., & Paseiro-Losada, P. (2002). High-performance liquid chromatographic determination of α-tocopherol in macroalgae. Journal of Chromatography A, 976(1-2), 277-284.
- SARAÇ, H., DAŞTAN, T., DEMİRBAŞ, A., DAŞTAN, S. D., KARAKÖY, T., & DURUKAN, H. Madımak (Polygonum cognatum Meissn.) Bitki Özütlerinin Besin Elementleri ve In Vitro Antikanserojen Aktiviteleri Yönünden Değerlendirilmesi. Ziraat Fakültesi Dergisi, 340-347.
- Shimada, K., Fujikawa, K., Yahara, K., & Nakamura, T. (1992). Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. Journal of agricultural and food chemistry, 40(6), 945-948.
- Shu, Y.-Z. (1998). Recent natural products based drug development: a pharmaceutical industry perspective. Journal of natural products, 61(8), 1053-1071.
- Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. M. (1999). [14] Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent Methods in enzymology (Vol. 299, pp. 152-178): Elsevier.
- Tatli, A. (1988). Important Range Plants of Erzurum Province. Food and Agriculture Organisation of the United Nations, Rome, 43.
- TOKUR, O., & AKSOY, A. (2017). In vitro sitotoksisite testleri. Harran Üniversitesi Veteriner Fakültesi Dergisi, 6(1), 112-118.
- Wu, L.-c., Chang, L.-H., Chen, S.-H., Fan, N.-c., & Ho, J.-a. A. (2009). Antioxidant activity and melanogenesis inhibitory effect of the acetonic extract of Osmanthus fragrans: A potential natural and functional food flavor additive. LWT-Food Science and Technology, 42(9), 1513-1519.
- Yalçın, A. S., Yılmaz, A. M., AltunDAğ, E. M., & KOçtürK, S. (2017). Kurkumin, kuersetin ve çay kateşinlerinin anti-kanser etkileri.
- Yıldırım, A., Mavi, A., & Kara, A. A. (2003). Antioxidant and antimicrobial activities of Polygonum cognatum Meissn extracts. Journal of the Science of Food and Agriculture, 83(1), 64-69.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Engineering |
| Journal Section | Articles |
| Authors | |
| Project Number | FEF.2018.00.002 |
| Publication Date | April 15, 2020 |
| Published in Issue | Year 2020 Issue: 18 |
