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Morinda reticulata Gamble Tubers: A Potent Hepatoprotective Agent Against CCl4 and Paracetamol Induced Hepatotoxicity in Wistar Albino Rats

Year 2020, Volume: 4 Issue: 1, 26 - 39, 01.06.2020

Raju Asirvatham Joshila Akhil

Abstract

Liver toxicityis the common issue in social life and it may induce by various chemicals and drugs. The hepatoprotective study was aimed to explore the extracts of Morinda reticulata Gamble for hepatoprotective activity against CCl4 and paracetamol induced hepatotoxicity on Wistar albino rats. Treatment group rats (4 groups, n=6 in each) were received either 200 mg/kg and 400 mg/kg of ethanol or aqueous extract of M. reticulata (p.o) and one group (n=6) was received 25 mg/kg of silymarin (p.o) twice daily for 7 days. CCl4 (1.5 ml/kg body weight, p.o) or paracetamol (2 g/kg) intoxication was done after the last dose of treatment and standard drug administration. Hepatoprotective potential was determined on the basis of measurement of hepatic serum marker enzymes such as Alanine Transaminase (AST), Aspartate transaminase (AST), Alkaline Phosphatase (ALP), Total bilirubin and liver weight. Further, liver tissue was subjected to histopathological studies. HPTLC studies were done to determine the presence of phytochemical constituents. Pretreatment with silymarin, ethanol and aqueous tuber extract of M. reticulata significantly (p < 0.001) reduced the CCl4 or paracetamol induced elevated AST, ALT, ALP, Total bilirubin level in serum and also reduced liver weight and total protein levels. HPTLC study of ethanol extract showed six distinct peaks for chemical constituents. The present study indicated that ethanol and aqueous tuber extract of M. reticulata has potential hepatoprotective effect against chemicals and it can be used as a clinical remedy for hepatotoxicity disorders.

Keywords

Hepatoprotective HPTLC fingerprint Morinda reticulata Gamble paracetamol sylimarin

Supporting Institution

SKCPRC, Parassala

Project Number

PG/005

Thanks

The authors gratefully acknowledge Dr. Sr. Betty Carla, Director and Dr. Vinod B, Principal, St. Joseph’s College of Pharmacy for providing support and facilities for this research work.

References

  • Akram, E., Pejman, M., Masoud, E. T., Ali, H. R., Shahabaldin, S. (2012). Hepatoprotective effects of pantothenic acid on carbontetrachloride induced toxicity in rats. EXCLI Journal, 11, 748-759.
  • Alharbi, N., Imam, F., Nadeem, A., Alharbi, M. M., Iqbal, M., Ahmad, S. F. (2014). Carbon tetrachloride induced hepatotoxicity in rat is reversed by treatment with riboflavin. International Immunopharmacology, 21 (2), 383-388.
  • Alhassan, A. J., Sule, M. S., Aliyu, S. A., Aliyu, M. D. (2009). Ideal hepatotoxicity model in rats using carbon tetrachloride (CCl4). Bayero Journal of Pure and Applied Sciences, 2 (2), 185-187.
  • Ashoush, I. S., El-Batawy, O. I., Gehan, A. (2013). Antioxidant activity and hepatoprotective effect of pomegranate pell and whey powder in rats. Annals of Agricultural Science, 58 (1), 27-32.
  • David, S., Hamilton, J. P. (2010). Drug-induced liver Injury. American Gastroenterology and Hepatology, 6, 73-80.
  • Deepak, K. D., Veerendra, C. Y., Siva, S. N., Tirtha, G., Rajalingamd, P. S., Bhim, C. M., Tapan, K. M. (2007). Evaluation of hepatoprotective and antioxidant activity of Lchnocarpus frutescens (Linn.) R.Br. on paracetamol induced hepatotoxicity in rats. Tropical Journal of Pharmaceutical Research, 6 (3), 755-765.
  • Garimamishra, R. l., Khosa, P. S., Jha, K. K. (2015). Hepatoprotective potential of ethanolic extract of Pandanus odoratissimus root against paracetamol-induced hepatotoxicity in rats. Journal of Pharmacy and Bioallied Science, 7 (1), 45-48.
  • Gini, C. K., Muraleedhara, K. G. (2008). Antioxidant activity of Aulosira fertilisimaon CCl4 induced hepatotoxicity in rats. Indian. Journal of Experimental Biology, 46, 52-59.
  • Howida, S., Abou, S. (2016). Physiological changes due to hepatotoxicity and protective role of some medicinal plants. Benisuef University Journal of Basic and Applied Sciences, 5, 134-146.
  • Ijinu, T. P, Anish, N., Shyju, H. (2011). Home gardens for nutrition and primary health security of rural poor of south Kerala. Indian Journal of Traditional Knowledge, 10 (3), 413-428.
  • Molehin, O. R., Oloyede, O. I., Idowu, K. A., Adeyanju, A. A., Olowoyeye, A. O., Tubi, O. I., Komolafe, O. E., Gold, A. S. (2017). White butterfly (Clerodendrum volubile) leaf extract protects against carbon tetrachloride induced hepatotoxicity in rats. Biomedicine and Pharmacotherapy, 96, 924-929.
  • Naik, S. R., Panda, V. S. (2008). Hepatoprotective effect of Ginkgo selectphytosome in Rifampicin induced liver injury in rats: evidence of antioxidant activity. Fitoterapia, 79, 439-45.
  • Navarro, V. J., Senior, J. R. (2006). Drug-related hepatotoxicity. New England Journal of Medicine, 354, 731-739.
  • Raju A., Joshila, A. (2017). Evaluation of in vitro and in vivo antioxidant potential of Morinda reticulata Gamble tubers in wistar albino rats subjected to ccl4 and paracetamol induced hepatotoxicity. Indonesian Journal of Pharmacy, 28 (3), 147-157.
  • Rease, G. E., Evans, W. C. (1989). A Textbook of Pharmacognosy. 13th edn. London; Bailliere Tindall Ltd.
  • Rotem, B. S., Yifei, C., Shishi, L., Catherine, H., Michael, R. H., Frederik, N. (2012). The biochemistry of acetaminophen hepatotoxicity and rescue: a mathematical model. Theoretical Biology and Medical Modelling, 9, 55.
  • Saumendu D. R., Sumit, D., Dibeyendu, S., Koushik, N. D. (2012). Herbal hepatoprotective agents. A review. World Journal of Pharmaceutical Research, 1 (2), 87-99.
  • Thabrew, M., Joice, P. A. (1987). Comparative study of the efficacy of Pavetta indica and Osbeckia octanda in the treatment of liver dysfunction. Planta Medica, 53 (3), 239-241.
  • Thangaraj, F. X., Moorthy, K., Leyone, L., Anthonysamy, A., Antony, K. F. R., Subburaman, S. K. (2014). Ethnobotanical study of Kani tribes in Thoduhills of Kerala, South India. Journal of Ethnopharmacology, 152, 78-90.
  • Uorakkottil, I., Deepshikha, P., Katare, V. A., Punnooth, P. N. (2016). A review on hepatoprotective and ımmunomodulatory herbal plants. Pharmacognosy Reviews, 10 (19), 66-70.
  • Xiaowen, L. I., Rong, Z., Boli., Mei, Z., Qing-Jian, S., Yepeng, Yang., Nan-Yin, H., Zai-Quan, L. I. (2010). Mechanism underlying carbon tetrachloride inhibited protein synthesis in liver. World Journal of Gastroenterology, 16 (31), 3950-3956.

Year 2020, Volume: 4 Issue: 1, 26 - 39, 01.06.2020

Raju Asirvatham Joshila Akhil

Abstract

Project Number

PG/005

References

  • Akram, E., Pejman, M., Masoud, E. T., Ali, H. R., Shahabaldin, S. (2012). Hepatoprotective effects of pantothenic acid on carbontetrachloride induced toxicity in rats. EXCLI Journal, 11, 748-759.
  • Alharbi, N., Imam, F., Nadeem, A., Alharbi, M. M., Iqbal, M., Ahmad, S. F. (2014). Carbon tetrachloride induced hepatotoxicity in rat is reversed by treatment with riboflavin. International Immunopharmacology, 21 (2), 383-388.
  • Alhassan, A. J., Sule, M. S., Aliyu, S. A., Aliyu, M. D. (2009). Ideal hepatotoxicity model in rats using carbon tetrachloride (CCl4). Bayero Journal of Pure and Applied Sciences, 2 (2), 185-187.
  • Ashoush, I. S., El-Batawy, O. I., Gehan, A. (2013). Antioxidant activity and hepatoprotective effect of pomegranate pell and whey powder in rats. Annals of Agricultural Science, 58 (1), 27-32.
  • David, S., Hamilton, J. P. (2010). Drug-induced liver Injury. American Gastroenterology and Hepatology, 6, 73-80.
  • Deepak, K. D., Veerendra, C. Y., Siva, S. N., Tirtha, G., Rajalingamd, P. S., Bhim, C. M., Tapan, K. M. (2007). Evaluation of hepatoprotective and antioxidant activity of Lchnocarpus frutescens (Linn.) R.Br. on paracetamol induced hepatotoxicity in rats. Tropical Journal of Pharmaceutical Research, 6 (3), 755-765.
  • Garimamishra, R. l., Khosa, P. S., Jha, K. K. (2015). Hepatoprotective potential of ethanolic extract of Pandanus odoratissimus root against paracetamol-induced hepatotoxicity in rats. Journal of Pharmacy and Bioallied Science, 7 (1), 45-48.
  • Gini, C. K., Muraleedhara, K. G. (2008). Antioxidant activity of Aulosira fertilisimaon CCl4 induced hepatotoxicity in rats. Indian. Journal of Experimental Biology, 46, 52-59.
  • Howida, S., Abou, S. (2016). Physiological changes due to hepatotoxicity and protective role of some medicinal plants. Benisuef University Journal of Basic and Applied Sciences, 5, 134-146.
  • Ijinu, T. P, Anish, N., Shyju, H. (2011). Home gardens for nutrition and primary health security of rural poor of south Kerala. Indian Journal of Traditional Knowledge, 10 (3), 413-428.
  • Molehin, O. R., Oloyede, O. I., Idowu, K. A., Adeyanju, A. A., Olowoyeye, A. O., Tubi, O. I., Komolafe, O. E., Gold, A. S. (2017). White butterfly (Clerodendrum volubile) leaf extract protects against carbon tetrachloride induced hepatotoxicity in rats. Biomedicine and Pharmacotherapy, 96, 924-929.
  • Naik, S. R., Panda, V. S. (2008). Hepatoprotective effect of Ginkgo selectphytosome in Rifampicin induced liver injury in rats: evidence of antioxidant activity. Fitoterapia, 79, 439-45.
  • Navarro, V. J., Senior, J. R. (2006). Drug-related hepatotoxicity. New England Journal of Medicine, 354, 731-739.
  • Raju A., Joshila, A. (2017). Evaluation of in vitro and in vivo antioxidant potential of Morinda reticulata Gamble tubers in wistar albino rats subjected to ccl4 and paracetamol induced hepatotoxicity. Indonesian Journal of Pharmacy, 28 (3), 147-157.
  • Rease, G. E., Evans, W. C. (1989). A Textbook of Pharmacognosy. 13th edn. London; Bailliere Tindall Ltd.
  • Rotem, B. S., Yifei, C., Shishi, L., Catherine, H., Michael, R. H., Frederik, N. (2012). The biochemistry of acetaminophen hepatotoxicity and rescue: a mathematical model. Theoretical Biology and Medical Modelling, 9, 55.
  • Saumendu D. R., Sumit, D., Dibeyendu, S., Koushik, N. D. (2012). Herbal hepatoprotective agents. A review. World Journal of Pharmaceutical Research, 1 (2), 87-99.
  • Thabrew, M., Joice, P. A. (1987). Comparative study of the efficacy of Pavetta indica and Osbeckia octanda in the treatment of liver dysfunction. Planta Medica, 53 (3), 239-241.
  • Thangaraj, F. X., Moorthy, K., Leyone, L., Anthonysamy, A., Antony, K. F. R., Subburaman, S. K. (2014). Ethnobotanical study of Kani tribes in Thoduhills of Kerala, South India. Journal of Ethnopharmacology, 152, 78-90.
  • Uorakkottil, I., Deepshikha, P., Katare, V. A., Punnooth, P. N. (2016). A review on hepatoprotective and ımmunomodulatory herbal plants. Pharmacognosy Reviews, 10 (19), 66-70.
  • Xiaowen, L. I., Rong, Z., Boli., Mei, Z., Qing-Jian, S., Yepeng, Yang., Nan-Yin, H., Zai-Quan, L. I. (2010). Mechanism underlying carbon tetrachloride inhibited protein synthesis in liver. World Journal of Gastroenterology, 16 (31), 3950-3956.
There are 21 citations in total.

Details

Primary Language English
Subjects Pharmacology and Pharmaceutical Sciences
Journal Section Research Article
Authors

Raju Asirvatham 0000-0002-7939-4975

Joshila Akhil This is me 0000-0002-9651-0464

Project Number PG/005
Publication Date June 1, 2020
Submission Date February 17, 2020
Acceptance Date May 12, 2020
Published in Issue Year 2020 Volume: 4 Issue: 1

Cite

APA Asirvatham, R., & Akhil, J. (2020). Morinda reticulata Gamble Tubers: A Potent Hepatoprotective Agent Against CCl4 and Paracetamol Induced Hepatotoxicity in Wistar Albino Rats. International Journal of Nature and Life Sciences, 4(1), 26-39.
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