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Serological and Virological Investigation of Bovine Viral Diarrhea Virus (BVDV) Infection in Candidate Bulls Before Taken in Artifical Insemination Centers by Enzyme Linked Immunosorbent Assay (ELISA)

Year 2013, Volume: 1 Issue: 2, 56 - 63, 02.02.2014

Sibel Yavru Oya Bulut Orhan Yapıcı Mehmet Kale Ayhan Ata

Abstract

Bovine Viral Diarrhea (BVD) is a common infection all over the world. It causes important economical losses in cattle breeding. In this study, a total of 46 blood samples were examined taken into tubes with and without EDTA from candidate bulls in Artifical Insemination Centers. Blood serum samples were tested to detect for antibodies against Bovine Viral Diarrhea Virus (BVDV) and leukocyte samples were tested for BVDV antigens by ELISA methods. Eight (17.3%) out of 46 serum samples were found as positive by the means of antibodies against BVDV while 3 (6.5%) out of 46 leukocyte samples were detected as positive for BVD antigens. One (2.1%) of the seropositive bulls was detected as positive for BVD antigen. Two (4.3%) of the seronegative bulls were detected as positive for BVD antigens.

Keywords

BVDV Bull ELISA

References

  • Bock RE, Burgess GW, Douglas IC. 1986. Development of an enzyme linked immunosorbent assay (ELISA) for the detection of bovine sera antibody to bovine viral diarrhoea virus. Aust. Vet. J. 63, 406-408. 2.
  • Brownlie J, Clarke MC, Howard CJ. 1984. Experimental production of fatal mucosal disease in cattle,.Vet. Rec. 114, 535-536. 3.
  • Brownlie J. 1991. The pathways for bovine diarrhoe virus biotypes in the pathogenesis of disease. Arch. Virol. Supp. 3, 79-96. 4.
  • Chu HJ, Zee CY, Ardans AA, et al. 1985. Enzyme-linked immunosorbent assay for the detection of antibodies to bovine viral diarrhea virus in bovine sera. Vet. Microbiol. 10, 325-333. 5.
  • Cornish TE, Van Olphen AL, Cavender JL, et. al. 2005. Comparison of ear
  • notch Reprod. Sci. 60-61, 615-627. 9.
  • Justewicz DM, Magar R, Marsolais G, et al. 1987. Bovine viral diarrhea virus-infected MDBK monolayer
  • immunosorbent assay (ELISA) for the measurment of antibodies in bovine sera. Vet. Immun. Immunopathol. 14, 377-384. in
  • enzyme-linked 10. Kale M, Yavru S, Ata A, ve ark. 2011. Bovine viral diarrhea virus (BVDV) infection in relation to fertility in heifers. J. Vet. Med. Sci. 73, 331-336.
  • Lamm CG, Broaddus CC, Holyoak GR et al. 2009. Distribution of bovine viral diarrhea virus antigen in aborted fetal and neonatal goats by immunohistochemistry. Vet. Pathol. 46, 54-58.
  • Marley MS, Tabor JM, Givens MD, et al. 2009. Bovine viral diarrhea virus is inactivated when whole milk from persistently infected cows is heated to prepare semen extender. Vet. Microbiol. 134, 249-253.
  • Mockeliuniene V, Salomskas A, Mockeliunas R, et al. 2004. Prevalence and epidemiological features of bovine viral diarrhoea virus infection in Lithuania. Vet. Microbiol. 99, 51-57.
  • Musoke AJ, Nantulya VM, Barbet AF, et al. 1981. Bovine
  • trypanosomes. Parasitol. Immunol. 3, 97-106. response to
  • Africa 15. Obando C, Baule C, Pedrique C, et al. 1999. Serological and molecular diagnosis of bovine viral diarrhoea virus and evidence of other viral infection in dairy calves with respiratory disease in Venezuela. Acta. Vet. Scand. 40, 253-262.
  • Ozturk D, Kale M, Pehlivanoglu F, et al. 2012. Evaluation for some bacterial and viral abortions of dairy cattle farms in Burdur district of Turkey. Kafkas Univ. Vet Fak. Derg. 18, 255-258.
  • Pacheco JM, Lager I. 2003. Indirect method ELISA for the detection of antibodies against bovine diarrhea virus in bovine serum. Rev. Argent. Microbiol. 35, 19-23.
  • Passler T, Walz P, Ditchkoff S, et al. 2007. Experimental persistent infection with bovine viral diarrhea virus in white-tailed deer. Vet. Microbiol. 122, 350-356.
  • Peterhans E, Schweizer M et al. 2010. Pestiviruses: How to outmaneuver your hosts. Vet. Microbiol. 142, 18-25.
  • Polak MP, Zmudzinski J. 1995. Prevalence of bovine viral diarrhea virus in the cattle population of Poland. Immunobiology of Viral Infections, Proc. 3rd Congress Europ. Soc. Vet. Virol. 309-310.
  • Radostits OM, Littlejohns IR. 1988. New concepts in the pathogenesis, diagnosis and control of diseases caused by the bovine viral diarrhea virus. Can. Vet. J. 29, 513-528.
  • Revell SG, Chasey D, Drew TW, et al. 1988. Some observations on the semen of bulls persistently infected with bovine virus diarrhoea virus. Vet. Rec. 123, 122-125.
  • Rossi CR, Bridgman CR, Kiesel GK. 1980. Viral contamination of bovine fetal lung cultures and bovine fetal sera. Am. J. Vet. Res. 41, 1680-1681.
  • Sandvik T, Fredriksen B, Loken T. 1997. Level of viral antigen in bloodleukocytefrom cattle acutely infected with bovine viral diarrhoes virus. J. Vet. Med. B. 44, 583-590.
  • Shannon AD, Richards SG, Kirkland PD, et al. 1991. An antigen-capture ELISA detects pestivirus antigens in blood and tissue of immunotolerant carrier cattle. J. Virol. Methods 34, 1-12.
  • Simsek A. 1997. Investigation of persistent bovine viral diarrhea virus infection in clinically healthy
  • importance. Vet. Bil. Derg. 13, 113-119. and its
  • epizootiologic 27. Tarry DW, Bernal L, Edwards S et al. 1991. Transmission of bovine virus diarrhoea virus by blood feding flies. Vet. Rec. 128, 82-84.
  • Tsvetkov P, Petkova K, Ognianov D. 1982. Serological study of the bovine virus diarrhea- mucosal disease. Vet. Med. Nauki. 19, 29-35.
  • Wasteway EG, Brinton M, Gaidamovich SY, et. al. 1985. Togaviridae, Intervirology 24, 125-139. 30. Westenbrink F, Middel WGJ, Straver PJ, et al. 1986. A blocking Enzyme-Linked Immunosorbent Assay (ELISA) for Bovine Virus Diarrhoea Virus Serology. J. Vet. Med. B. 33, 354-361.
  • Westenbrink F, Straver PJ, Kimman TG, et al. 1989. Development of a neutralising antibody response to an inoculated cytopathogenic strain of bovine virus diarrhoe virus. Vet. Rec. 125, 262-265. 32. Xia H, Vijayaraghavan B, Belak S, et al. 2011. Detection and Identification of the Atypical Bovine Pestiviruses in Commercial Foetal Bovine Serum Batches, PloS One, 6, e28553.
  • Zemke J, Knig P, Mischkale K, et al. 2010. Novel BVDV-2 mutants as new candidates for modified-live vaccines. Vet. Microbiol. 142, 69-80.

Suni Tohumlama Merkezlerine Alınma Öncesi Aday Boğalarda Enzyme Linked Immunosorbent Assay (ELISA) ile Bovine Viral Diarrhea Virus (BVDV) Enfeksiyonunun Serolojik ve Virolojik Olarak Araştırılması

Year 2013, Volume: 1 Issue: 2, 56 - 63, 02.02.2014

Sibel Yavru Oya Bulut Orhan Yapıcı Mehmet Kale Ayhan Ata

Abstract

Bovine Viral Diarrhea (BVD) tüm dünya üzerinde görülen yaygın bir enfeksiyondur. Sığır yetiĢtiriciliğindeki en önemli ekonomik kayıpların nedenidir. Bu çalıĢmada suni tohumlama merkezlerinde kullanılmak üzere seçilen toplam 46 adet aday boğadan EDTA‟lı ve EDTA‟sız tüplere kan örnekleri alınarak incelendi. Kan serum örnekleri BVDV‟ye karĢı oluĢan antikorlar yönünden, lökosit örnekleri ise BVDV antijen varlığı yönünden ELISA
ile test edildi. 46 adet serum örneğinden 8 (%17.3)
adedi BVDV antikorları yönünden seropozitif olarak
tespit edilirken, lökosit örneklerinden 3 adedi (%6.5)
ise BVDV antijen varlığı yönünden pozitif bulundu. 3
adet BVDV antijen pozitif boğanın 2 adedi (%4.3)
seronegatif iken 1 adedi (%2.1) ise seropozitif olarak
tespit edildi.

Keywords

BVDV Boğa ELISA

References

  • Bock RE, Burgess GW, Douglas IC. 1986. Development of an enzyme linked immunosorbent assay (ELISA) for the detection of bovine sera antibody to bovine viral diarrhoea virus. Aust. Vet. J. 63, 406-408. 2.
  • Brownlie J, Clarke MC, Howard CJ. 1984. Experimental production of fatal mucosal disease in cattle,.Vet. Rec. 114, 535-536. 3.
  • Brownlie J. 1991. The pathways for bovine diarrhoe virus biotypes in the pathogenesis of disease. Arch. Virol. Supp. 3, 79-96. 4.
  • Chu HJ, Zee CY, Ardans AA, et al. 1985. Enzyme-linked immunosorbent assay for the detection of antibodies to bovine viral diarrhea virus in bovine sera. Vet. Microbiol. 10, 325-333. 5.
  • Cornish TE, Van Olphen AL, Cavender JL, et. al. 2005. Comparison of ear
  • notch Reprod. Sci. 60-61, 615-627. 9.
  • Justewicz DM, Magar R, Marsolais G, et al. 1987. Bovine viral diarrhea virus-infected MDBK monolayer
  • immunosorbent assay (ELISA) for the measurment of antibodies in bovine sera. Vet. Immun. Immunopathol. 14, 377-384. in
  • enzyme-linked 10. Kale M, Yavru S, Ata A, ve ark. 2011. Bovine viral diarrhea virus (BVDV) infection in relation to fertility in heifers. J. Vet. Med. Sci. 73, 331-336.
  • Lamm CG, Broaddus CC, Holyoak GR et al. 2009. Distribution of bovine viral diarrhea virus antigen in aborted fetal and neonatal goats by immunohistochemistry. Vet. Pathol. 46, 54-58.
  • Marley MS, Tabor JM, Givens MD, et al. 2009. Bovine viral diarrhea virus is inactivated when whole milk from persistently infected cows is heated to prepare semen extender. Vet. Microbiol. 134, 249-253.
  • Mockeliuniene V, Salomskas A, Mockeliunas R, et al. 2004. Prevalence and epidemiological features of bovine viral diarrhoea virus infection in Lithuania. Vet. Microbiol. 99, 51-57.
  • Musoke AJ, Nantulya VM, Barbet AF, et al. 1981. Bovine
  • trypanosomes. Parasitol. Immunol. 3, 97-106. response to
  • Africa 15. Obando C, Baule C, Pedrique C, et al. 1999. Serological and molecular diagnosis of bovine viral diarrhoea virus and evidence of other viral infection in dairy calves with respiratory disease in Venezuela. Acta. Vet. Scand. 40, 253-262.
  • Ozturk D, Kale M, Pehlivanoglu F, et al. 2012. Evaluation for some bacterial and viral abortions of dairy cattle farms in Burdur district of Turkey. Kafkas Univ. Vet Fak. Derg. 18, 255-258.
  • Pacheco JM, Lager I. 2003. Indirect method ELISA for the detection of antibodies against bovine diarrhea virus in bovine serum. Rev. Argent. Microbiol. 35, 19-23.
  • Passler T, Walz P, Ditchkoff S, et al. 2007. Experimental persistent infection with bovine viral diarrhea virus in white-tailed deer. Vet. Microbiol. 122, 350-356.
  • Peterhans E, Schweizer M et al. 2010. Pestiviruses: How to outmaneuver your hosts. Vet. Microbiol. 142, 18-25.
  • Polak MP, Zmudzinski J. 1995. Prevalence of bovine viral diarrhea virus in the cattle population of Poland. Immunobiology of Viral Infections, Proc. 3rd Congress Europ. Soc. Vet. Virol. 309-310.
  • Radostits OM, Littlejohns IR. 1988. New concepts in the pathogenesis, diagnosis and control of diseases caused by the bovine viral diarrhea virus. Can. Vet. J. 29, 513-528.
  • Revell SG, Chasey D, Drew TW, et al. 1988. Some observations on the semen of bulls persistently infected with bovine virus diarrhoea virus. Vet. Rec. 123, 122-125.
  • Rossi CR, Bridgman CR, Kiesel GK. 1980. Viral contamination of bovine fetal lung cultures and bovine fetal sera. Am. J. Vet. Res. 41, 1680-1681.
  • Sandvik T, Fredriksen B, Loken T. 1997. Level of viral antigen in bloodleukocytefrom cattle acutely infected with bovine viral diarrhoes virus. J. Vet. Med. B. 44, 583-590.
  • Shannon AD, Richards SG, Kirkland PD, et al. 1991. An antigen-capture ELISA detects pestivirus antigens in blood and tissue of immunotolerant carrier cattle. J. Virol. Methods 34, 1-12.
  • Simsek A. 1997. Investigation of persistent bovine viral diarrhea virus infection in clinically healthy
  • importance. Vet. Bil. Derg. 13, 113-119. and its
  • epizootiologic 27. Tarry DW, Bernal L, Edwards S et al. 1991. Transmission of bovine virus diarrhoea virus by blood feding flies. Vet. Rec. 128, 82-84.
  • Tsvetkov P, Petkova K, Ognianov D. 1982. Serological study of the bovine virus diarrhea- mucosal disease. Vet. Med. Nauki. 19, 29-35.
  • Wasteway EG, Brinton M, Gaidamovich SY, et. al. 1985. Togaviridae, Intervirology 24, 125-139. 30. Westenbrink F, Middel WGJ, Straver PJ, et al. 1986. A blocking Enzyme-Linked Immunosorbent Assay (ELISA) for Bovine Virus Diarrhoea Virus Serology. J. Vet. Med. B. 33, 354-361.
  • Westenbrink F, Straver PJ, Kimman TG, et al. 1989. Development of a neutralising antibody response to an inoculated cytopathogenic strain of bovine virus diarrhoe virus. Vet. Rec. 125, 262-265. 32. Xia H, Vijayaraghavan B, Belak S, et al. 2011. Detection and Identification of the Atypical Bovine Pestiviruses in Commercial Foetal Bovine Serum Batches, PloS One, 6, e28553.
  • Zemke J, Knig P, Mischkale K, et al. 2010. Novel BVDV-2 mutants as new candidates for modified-live vaccines. Vet. Microbiol. 142, 69-80.
There are 32 citations in total.

Details

Primary Language English
Journal Section Makaleler
Authors

Sibel Yavru This is me

Oya Bulut This is me

Orhan Yapıcı This is me

Mehmet Kale

Ayhan Ata This is me

Publication Date February 2, 2014
Submission Date March 10, 2013
Published in Issue Year 2013 Volume: 1 Issue: 2

Cite

APA Yavru, S., Bulut, O., Yapıcı, O., Kale, M., et al. (2014). Serological and Virological Investigation of Bovine Viral Diarrhea Virus (BVDV) Infection in Candidate Bulls Before Taken in Artifical Insemination Centers by Enzyme Linked Immunosorbent Assay (ELISA). Mehmet Akif Ersoy University Journal of Health Sciences Institute, 1(2), 56-63.
AMA Yavru S, Bulut O, Yapıcı O, Kale M, Ata A. Serological and Virological Investigation of Bovine Viral Diarrhea Virus (BVDV) Infection in Candidate Bulls Before Taken in Artifical Insemination Centers by Enzyme Linked Immunosorbent Assay (ELISA). Mehmet Akif Ersoy University Journal of Health Sciences Institute. February 2014;1(2):56-63.
Chicago Yavru, Sibel, Oya Bulut, Orhan Yapıcı, Mehmet Kale, and Ayhan Ata. “Serological and Virological Investigation of Bovine Viral Diarrhea Virus (BVDV) Infection in Candidate Bulls Before Taken in Artifical Insemination Centers by Enzyme Linked Immunosorbent Assay (ELISA)”. Mehmet Akif Ersoy University Journal of Health Sciences Institute 1, no. 2 (February 2014): 56-63.
EndNote Yavru S, Bulut O, Yapıcı O, Kale M, Ata A (February 1, 2014) Serological and Virological Investigation of Bovine Viral Diarrhea Virus (BVDV) Infection in Candidate Bulls Before Taken in Artifical Insemination Centers by Enzyme Linked Immunosorbent Assay (ELISA). Mehmet Akif Ersoy University Journal of Health Sciences Institute 1 2 56–63.
IEEE S. Yavru, O. Bulut, O. Yapıcı, M. Kale, and A. Ata, “Serological and Virological Investigation of Bovine Viral Diarrhea Virus (BVDV) Infection in Candidate Bulls Before Taken in Artifical Insemination Centers by Enzyme Linked Immunosorbent Assay (ELISA)”, Mehmet Akif Ersoy University Journal of Health Sciences Institute, vol. 1, no. 2, pp. 56–63, 2014.
ISNAD Yavru, Sibel et al. “Serological and Virological Investigation of Bovine Viral Diarrhea Virus (BVDV) Infection in Candidate Bulls Before Taken in Artifical Insemination Centers by Enzyme Linked Immunosorbent Assay (ELISA)”. Mehmet Akif Ersoy University Journal of Health Sciences Institute 1/2 (February 2014), 56-63.
JAMA Yavru S, Bulut O, Yapıcı O, Kale M, Ata A. Serological and Virological Investigation of Bovine Viral Diarrhea Virus (BVDV) Infection in Candidate Bulls Before Taken in Artifical Insemination Centers by Enzyme Linked Immunosorbent Assay (ELISA). Mehmet Akif Ersoy University Journal of Health Sciences Institute. 2014;1:56–63.
MLA Yavru, Sibel et al. “Serological and Virological Investigation of Bovine Viral Diarrhea Virus (BVDV) Infection in Candidate Bulls Before Taken in Artifical Insemination Centers by Enzyme Linked Immunosorbent Assay (ELISA)”. Mehmet Akif Ersoy University Journal of Health Sciences Institute, vol. 1, no. 2, 2014, pp. 56-63.
Vancouver Yavru S, Bulut O, Yapıcı O, Kale M, Ata A. Serological and Virological Investigation of Bovine Viral Diarrhea Virus (BVDV) Infection in Candidate Bulls Before Taken in Artifical Insemination Centers by Enzyme Linked Immunosorbent Assay (ELISA). Mehmet Akif Ersoy University Journal of Health Sciences Institute. 2014;1(2):56-63.
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