Morinda reticulata Gamble Tubers: A Potent Hepatoprotective Agent Against CCl4 and Paracetamol Induced Hepatotoxicity in Wistar Albino Rats
Öz
Liver toxicityis the common issue in social life and it may induce by various chemicals and drugs. The hepatoprotective study was aimed to explore the extracts of Morinda reticulata Gamble for hepatoprotective activity against CCl4 and paracetamol induced hepatotoxicity on Wistar albino rats. Treatment group rats (4 groups, n=6 in each) were received either 200 mg/kg and 400 mg/kg of ethanol or aqueous extract of M. reticulata (p.o) and one group (n=6) was received 25 mg/kg of silymarin (p.o) twice daily for 7 days. CCl4 (1.5 ml/kg body weight, p.o) or paracetamol (2 g/kg) intoxication was done after the last dose of treatment and standard drug administration. Hepatoprotective potential was determined on the basis of measurement of hepatic serum marker enzymes such as Alanine Transaminase (AST), Aspartate transaminase (AST), Alkaline Phosphatase (ALP), Total bilirubin and liver weight. Further, liver tissue was subjected to histopathological studies. HPTLC studies were done to determine the presence of phytochemical constituents. Pretreatment with silymarin, ethanol and aqueous tuber extract of M. reticulata significantly (p < 0.001) reduced the CCl4 or paracetamol induced elevated AST, ALT, ALP, Total bilirubin level in serum and also reduced liver weight and total protein levels. HPTLC study of ethanol extract showed six distinct peaks for chemical constituents. The present study indicated that ethanol and aqueous tuber extract of M. reticulata has potential hepatoprotective effect against chemicals and it can be used as a clinical remedy for hepatotoxicity disorders.
Anahtar Kelimeler
Hepatoprotective HPTLC fingerprint Morinda reticulata Gamble paracetamol sylimarin
Destekleyen Kurum
SKCPRC, Parassala
Proje Numarası
PG/005
Teşekkür
The authors gratefully acknowledge Dr. Sr. Betty Carla, Director and Dr. Vinod B, Principal, St. Joseph’s College of Pharmacy for providing support and facilities for this research work.
Kaynakça
- Akram, E., Pejman, M., Masoud, E. T., Ali, H. R., Shahabaldin, S. (2012). Hepatoprotective effects of pantothenic acid on carbontetrachloride induced toxicity in rats. EXCLI Journal, 11, 748-759.
- Alharbi, N., Imam, F., Nadeem, A., Alharbi, M. M., Iqbal, M., Ahmad, S. F. (2014). Carbon tetrachloride induced hepatotoxicity in rat is reversed by treatment with riboflavin. International Immunopharmacology, 21 (2), 383-388.
- Alhassan, A. J., Sule, M. S., Aliyu, S. A., Aliyu, M. D. (2009). Ideal hepatotoxicity model in rats using carbon tetrachloride (CCl4). Bayero Journal of Pure and Applied Sciences, 2 (2), 185-187.
- Ashoush, I. S., El-Batawy, O. I., Gehan, A. (2013). Antioxidant activity and hepatoprotective effect of pomegranate pell and whey powder in rats. Annals of Agricultural Science, 58 (1), 27-32.
- David, S., Hamilton, J. P. (2010). Drug-induced liver Injury. American Gastroenterology and Hepatology, 6, 73-80.
- Deepak, K. D., Veerendra, C. Y., Siva, S. N., Tirtha, G., Rajalingamd, P. S., Bhim, C. M., Tapan, K. M. (2007). Evaluation of hepatoprotective and antioxidant activity of Lchnocarpus frutescens (Linn.) R.Br. on paracetamol induced hepatotoxicity in rats. Tropical Journal of Pharmaceutical Research, 6 (3), 755-765.
- Garimamishra, R. l., Khosa, P. S., Jha, K. K. (2015). Hepatoprotective potential of ethanolic extract of Pandanus odoratissimus root against paracetamol-induced hepatotoxicity in rats. Journal of Pharmacy and Bioallied Science, 7 (1), 45-48.
- Gini, C. K., Muraleedhara, K. G. (2008). Antioxidant activity of Aulosira fertilisimaon CCl4 induced hepatotoxicity in rats. Indian. Journal of Experimental Biology, 46, 52-59.
- Howida, S., Abou, S. (2016). Physiological changes due to hepatotoxicity and protective role of some medicinal plants. Benisuef University Journal of Basic and Applied Sciences, 5, 134-146.
- Ijinu, T. P, Anish, N., Shyju, H. (2011). Home gardens for nutrition and primary health security of rural poor of south Kerala. Indian Journal of Traditional Knowledge, 10 (3), 413-428.
- Molehin, O. R., Oloyede, O. I., Idowu, K. A., Adeyanju, A. A., Olowoyeye, A. O., Tubi, O. I., Komolafe, O. E., Gold, A. S. (2017). White butterfly (Clerodendrum volubile) leaf extract protects against carbon tetrachloride induced hepatotoxicity in rats. Biomedicine and Pharmacotherapy, 96, 924-929.
- Naik, S. R., Panda, V. S. (2008). Hepatoprotective effect of Ginkgo selectphytosome in Rifampicin induced liver injury in rats: evidence of antioxidant activity. Fitoterapia, 79, 439-45.
- Navarro, V. J., Senior, J. R. (2006). Drug-related hepatotoxicity. New England Journal of Medicine, 354, 731-739.
- Raju A., Joshila, A. (2017). Evaluation of in vitro and in vivo antioxidant potential of Morinda reticulata Gamble tubers in wistar albino rats subjected to ccl4 and paracetamol induced hepatotoxicity. Indonesian Journal of Pharmacy, 28 (3), 147-157.
- Rease, G. E., Evans, W. C. (1989). A Textbook of Pharmacognosy. 13th edn. London; Bailliere Tindall Ltd.
- Rotem, B. S., Yifei, C., Shishi, L., Catherine, H., Michael, R. H., Frederik, N. (2012). The biochemistry of acetaminophen hepatotoxicity and rescue: a mathematical model. Theoretical Biology and Medical Modelling, 9, 55.
- Saumendu D. R., Sumit, D., Dibeyendu, S., Koushik, N. D. (2012). Herbal hepatoprotective agents. A review. World Journal of Pharmaceutical Research, 1 (2), 87-99.
- Thabrew, M., Joice, P. A. (1987). Comparative study of the efficacy of Pavetta indica and Osbeckia octanda in the treatment of liver dysfunction. Planta Medica, 53 (3), 239-241.
- Thangaraj, F. X., Moorthy, K., Leyone, L., Anthonysamy, A., Antony, K. F. R., Subburaman, S. K. (2014). Ethnobotanical study of Kani tribes in Thoduhills of Kerala, South India. Journal of Ethnopharmacology, 152, 78-90.
- Uorakkottil, I., Deepshikha, P., Katare, V. A., Punnooth, P. N. (2016). A review on hepatoprotective and ımmunomodulatory herbal plants. Pharmacognosy Reviews, 10 (19), 66-70.
- Xiaowen, L. I., Rong, Z., Boli., Mei, Z., Qing-Jian, S., Yepeng, Yang., Nan-Yin, H., Zai-Quan, L. I. (2010). Mechanism underlying carbon tetrachloride inhibited protein synthesis in liver. World Journal of Gastroenterology, 16 (31), 3950-3956.
Öz
Proje Numarası
PG/005
Kaynakça
- Akram, E., Pejman, M., Masoud, E. T., Ali, H. R., Shahabaldin, S. (2012). Hepatoprotective effects of pantothenic acid on carbontetrachloride induced toxicity in rats. EXCLI Journal, 11, 748-759.
- Alharbi, N., Imam, F., Nadeem, A., Alharbi, M. M., Iqbal, M., Ahmad, S. F. (2014). Carbon tetrachloride induced hepatotoxicity in rat is reversed by treatment with riboflavin. International Immunopharmacology, 21 (2), 383-388.
- Alhassan, A. J., Sule, M. S., Aliyu, S. A., Aliyu, M. D. (2009). Ideal hepatotoxicity model in rats using carbon tetrachloride (CCl4). Bayero Journal of Pure and Applied Sciences, 2 (2), 185-187.
- Ashoush, I. S., El-Batawy, O. I., Gehan, A. (2013). Antioxidant activity and hepatoprotective effect of pomegranate pell and whey powder in rats. Annals of Agricultural Science, 58 (1), 27-32.
- David, S., Hamilton, J. P. (2010). Drug-induced liver Injury. American Gastroenterology and Hepatology, 6, 73-80.
- Deepak, K. D., Veerendra, C. Y., Siva, S. N., Tirtha, G., Rajalingamd, P. S., Bhim, C. M., Tapan, K. M. (2007). Evaluation of hepatoprotective and antioxidant activity of Lchnocarpus frutescens (Linn.) R.Br. on paracetamol induced hepatotoxicity in rats. Tropical Journal of Pharmaceutical Research, 6 (3), 755-765.
- Garimamishra, R. l., Khosa, P. S., Jha, K. K. (2015). Hepatoprotective potential of ethanolic extract of Pandanus odoratissimus root against paracetamol-induced hepatotoxicity in rats. Journal of Pharmacy and Bioallied Science, 7 (1), 45-48.
- Gini, C. K., Muraleedhara, K. G. (2008). Antioxidant activity of Aulosira fertilisimaon CCl4 induced hepatotoxicity in rats. Indian. Journal of Experimental Biology, 46, 52-59.
- Howida, S., Abou, S. (2016). Physiological changes due to hepatotoxicity and protective role of some medicinal plants. Benisuef University Journal of Basic and Applied Sciences, 5, 134-146.
- Ijinu, T. P, Anish, N., Shyju, H. (2011). Home gardens for nutrition and primary health security of rural poor of south Kerala. Indian Journal of Traditional Knowledge, 10 (3), 413-428.
- Molehin, O. R., Oloyede, O. I., Idowu, K. A., Adeyanju, A. A., Olowoyeye, A. O., Tubi, O. I., Komolafe, O. E., Gold, A. S. (2017). White butterfly (Clerodendrum volubile) leaf extract protects against carbon tetrachloride induced hepatotoxicity in rats. Biomedicine and Pharmacotherapy, 96, 924-929.
- Naik, S. R., Panda, V. S. (2008). Hepatoprotective effect of Ginkgo selectphytosome in Rifampicin induced liver injury in rats: evidence of antioxidant activity. Fitoterapia, 79, 439-45.
- Navarro, V. J., Senior, J. R. (2006). Drug-related hepatotoxicity. New England Journal of Medicine, 354, 731-739.
- Raju A., Joshila, A. (2017). Evaluation of in vitro and in vivo antioxidant potential of Morinda reticulata Gamble tubers in wistar albino rats subjected to ccl4 and paracetamol induced hepatotoxicity. Indonesian Journal of Pharmacy, 28 (3), 147-157.
- Rease, G. E., Evans, W. C. (1989). A Textbook of Pharmacognosy. 13th edn. London; Bailliere Tindall Ltd.
- Rotem, B. S., Yifei, C., Shishi, L., Catherine, H., Michael, R. H., Frederik, N. (2012). The biochemistry of acetaminophen hepatotoxicity and rescue: a mathematical model. Theoretical Biology and Medical Modelling, 9, 55.
- Saumendu D. R., Sumit, D., Dibeyendu, S., Koushik, N. D. (2012). Herbal hepatoprotective agents. A review. World Journal of Pharmaceutical Research, 1 (2), 87-99.
- Thabrew, M., Joice, P. A. (1987). Comparative study of the efficacy of Pavetta indica and Osbeckia octanda in the treatment of liver dysfunction. Planta Medica, 53 (3), 239-241.
- Thangaraj, F. X., Moorthy, K., Leyone, L., Anthonysamy, A., Antony, K. F. R., Subburaman, S. K. (2014). Ethnobotanical study of Kani tribes in Thoduhills of Kerala, South India. Journal of Ethnopharmacology, 152, 78-90.
- Uorakkottil, I., Deepshikha, P., Katare, V. A., Punnooth, P. N. (2016). A review on hepatoprotective and ımmunomodulatory herbal plants. Pharmacognosy Reviews, 10 (19), 66-70.
- Xiaowen, L. I., Rong, Z., Boli., Mei, Z., Qing-Jian, S., Yepeng, Yang., Nan-Yin, H., Zai-Quan, L. I. (2010). Mechanism underlying carbon tetrachloride inhibited protein synthesis in liver. World Journal of Gastroenterology, 16 (31), 3950-3956.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Eczacılık ve İlaç Bilimleri |
| Bölüm | Araştırma Makalesi |
| Yazarlar | |
| Proje Numarası | PG/005 |
| Yayımlanma Tarihi | 1 Haziran 2020 |
| Gönderilme Tarihi | 17 Şubat 2020 |
| Kabul Tarihi | 12 Mayıs 2020 |
| Yayımlandığı Sayı | Yıl 2020 Cilt: 4 Sayı: 1 |
