Araştırma Makalesi
Yıl 2018,
Cilt: 1 Sayı: 1, 5 - 8, 22.12.2018
Öz
Kulak zarının temel fonksiyonu ses titreşimlerini dış ortamdan alarak kulak kemikçiklerine iletmektir. Yara iyileşmesinde mast hücrelerinin sayılarının arttığı bildirilmektedir. Bu çalışmada, deneysel pars tensa perforasyonunda mast hücrelerinin sayı ve heterojenitesi incelenmiştir. Pars tensa %10 formol’de tespit edilip yıkandıktan sonra rutin histolojik doku takibi prosedürlerinden geçirilerek parafinde bloklandı. Alınan kesitler toluidin blue ve alcian blue/safranin O kombine boya metodu ile boyandı. Tüm gruplarda, pars tensada mast hücreleri metakromatik, alcian blue (+) ve safranin O (+) boyandı. İyileşme sürecinde en fazla sayıda mast hücresine nar ekstratı verilmiş grupta rastlandı.
Anahtar Kelimeler
Kaynakça
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- Santa Maria PL, Redmond SL, Atlas MD, Ghassemifar R. 2010. Histology of the healing tympanic membrane following perforation in rats. Laryngoscope, 120(10): 206170.
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- Krystel-Whittemore M, Dileepan KN, Wood JG. 2016. Mast cell: a multi- functional master cell. Frontiers in Immunology, 6, 620.
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- Wulff BC, Parent AE, Meleski MA, DiPietro LA, Schrementi ME, Wilgus TA. 2012. Mast cells contribute to scar formation during fetal wound healing. Journal of Investigative Dermatology, 132(2): 458-65.
- Crossmon O. 1937. A modifi cation of mallory’s connective tissue stain with a discussion of the principles ınvolued. The Anatomical Record, 69: 31-38.
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- Ertuğrul T, Çiftci G, Tütüncü Ş. 2017. Formaldehite maruz kalmış ratlarda böbrek dokusunda mast hücrelerinin dağılımı ve heterojenitesi. Veteriner Hekimler Derneği Dergisi, 88(2): 26-37.
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- Mattsson C, Johansson C, Hellström S. 1999. Myringosclerosis develops within 9h of myringotomy. Otorhinolaryngol Relat Spec, 61 (1): 31-6.
- Eriksson PO, Mattsson C, Hellstrom S. 2003. First forty-eight hours of developing otitis media: An experimental study. Annals of Otology, Rhinology & Laryngology, 112, 558-66.
- Spratley JE, Hellström SO, Mattsson CK, Pais-Clemente M. 2001. Topical ascorbic acid reduces myringosclerosis in perforated tympanic membranes. A study in the rat. Annals of Otology, Rhinology & Laryngology, 110 (6): 585-91.
- Spratley J, Hellström S, Eriksson PO, Pais-Clemente M. 2002. Myringotomy delays the tympanic membrane recovery in acute otitis media: a study in the rat model. Laryngoscope, 112: 1474-81.
- Santa Maria PL, Redmond SL, Atlas M.D, Ghassemifar R. 2010. Histology of the healing tympanic membrane following perforation in rats. Laryngoscope, 120 (10): 2061-70.
- Schmidt SH, Hellström S, Carlsöö B. 1984. Shortterm effect of local anaesthetic agents on the structure of the rat tympanic membrane. Arch Otorhinolaryngology, 240: 159-166.
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- Cayé-Thomasen P, Tos M. 1996. Mast cell clusters in pars tensa membranae tympani in acute otitis media: a possible role in perforation healing. Acta Oto-Laryngologica, 116(6): 845-9.
- Watanabe T, Kawauchi H, Fujiyoshi T, Mogi G. 1991. Distribution of mast cells in the tubotympanum of guinea pigs. Annals of Otology, Rhinology & Laryngology, 100: 407-12.
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- Eren U, Kum S, Sandıkcı M, Kara E. 2006. Effects of long-term passive smoking on the mast cells in rat lungs. Revue de Médecine Vétérinaire, 157, 6, 319-322.
Toplam 29 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Veteriner Cerrahi |
| Bölüm | Makaleler |
| Yazarlar | |
| Yayımlanma Tarihi | 22 Aralık 2018 |
| Yayımlandığı Sayı | Yıl 2018 Cilt: 1 Sayı: 1 |