Araştırma Makalesi
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HELIOTROPIUM HIRSUTISSIMUM, HELIOTROPIUM DOLOSUM VE HELIOTROPIUM LASIOCARPUM METANOL EKSTRELERİNİN FAREDE PENTİLENTETRAZOL İNDÜKLÜ NÖBETLERE ETKİSİ

Yıl 2018, Cilt: 42 Sayı: 3, 13 - 19, 30.09.2018

Okan Arıhan Songul Karakaya Ceyda Sibel Kılıc Ozlem Ergul Erkec Mehmet Kara Hayri Duman

Öz

Amaç: Epilepsi antik dönemlerden bu yana bilinen yaygın bir nörolojik problemdir. Epilepsinin modern tedavisi nöbetleri engelleyici ilaçlar ile yapılmaktadır. İlaç tedavisine dirençli hastaların varlığı nedeniyle yeni antiepileptik ilaçların arayışı devam etmektedir. Bitkiler yeni ilaçların bulunabilmesi için önemli bir kaynaktır. Bu çalışmada Heliotropium (Türkçe; Akrep otu, Siğil otu) türlerinin; Heliotropium hirsutissimum Grauer, H. dolosum De Not ve H. lasiocarpum Fisch. et Mey.’in faredeki etkilerini değerlendirmeyi amaçladık. 

Gereç ve Yöntem: Nöbetlerin oluşması için PTZ (pentilentetrazol) kullanıldı. Deney grupları PTZ ve bahsedilen 3 Heliotropium türünün ekstreleri+PTZ olarak belirlendi. 

Sonuç ve Tartışma: H. hirsutissimum ve H. lasiocarpum gruplarında, tonik-klonik nöbete giren hayvan sayısı PTZ ve H. dolosum gruplarına göre azaldı ancak istatistik anlamlılık gözlenmedi. İlk miyoklonik nöbet ve ilk tonik-klonik nöbet için gereken süre tüm Heliotropium gruplarında PTZ grubuna göre uzadı (p>0.05). Bu tonik klonik nöbetler sonrasında ölen hayvan sayısı tüm Heliotropium gruplarında azaldı (p>0.05). Tonikklonik nöbet süresi H. hirsutissimum ve H. lasiocarpum gruplarında azaldı (p>0.05). Sonuçlar birlikte değerlendirildiğinde Heliotropium türlerinin antikonvülsif olarak etnobotanik kullanımının bir karşılığını olabileceğini düşündürmektedir.

Anahtar Kelimeler

epilepsy Heliotropium nöbet PTZ pentilentetrazol

Kaynakça

  • Baranano, K., Hartman, A. (2008). The Ketogenic Diet: Uses in Epilepsy and Other Neurologic Illnesses. Current Treatment Options in Neurology, 10, 410-419.
  • Eadie, M.J. (2004). Could valerian have been the first anticonvulsant? Epilepsia, 45, 1338-1343.
  • Yapıcı, Ü., Hoşgören, H., Saya, Ö. (2009). Kurtalan (Siirt) İlçesinin Etnobotanik Özellikleri. Dicle Üniversitesi Ziya Gökalp Eğitim Fakültesi Dergisi, 12, 191-196.
  • Tuzlacı, E., Doğan, A. (2010). Turkish folk medicinal plants, IX: Ovacık (Tunceli). Marmara Pharmaceutical Journal, 14, 136-143.
  • Sarı, A.O., Oğuz, B., Bilgiç, A., Tort, N., Günseven. A., Şenol, S.G. (2010). Ege ve Güney Marmara Bölgelerinde Halk İlacı Olarak Kullanılan Bitkiler. Anadolu Journal of AARI, 20(2), 1-21.
  • Melikoğlu, R.G., Kurtoğlu, S. (2015). Türkiye’de Astım Tedavisinde Geleneksel Olarak Kullanılan Bitkiler. Marmara Pharmaceutical Journal, 19, 1-11.
  • Singh, B., Sharma, R.A. (2015). Anti-Inflammatory and Antimicrobial Properties of Flavonoids from Heliotropium subulatum Exudate. Inflammation & Allergy Drug Targets, 14(2), 125-132.
  • Khan, H., Khan, M.A., Gul, F., Hussain, S., Ashraf, N. (2015). Anti-inflammatory activity of Heliotropium strigosum in animal models. Toxicology and Industrial Health, 31, 1281-1287.
  • Janbaz, K.H., Javed, S., Saqib, F., Imran, I., Zia-Ul-Haq, M., De Feo, V. (2015). Validation of ethnopharmacological uses of Heliotropium strigosum Willd. as spasmolytic, bronchodilator and vasorelaxant remedy. BMC Complementary and Alternative Medicine, 15, 169.
  • Murugesh, K., Yeligar, V., Dash, D.K., Sengupta, P., Maiti, B.C., Maity, T.K. (2006). Antidiabetic, antioxidant and antihyperlipidemic status of Heliotropium zeylanicum extract on streptozotocin-induced diabetes in rats. Biological and Pharmaceutical Bulletin, 29, 2202-2205.
  • Ghaffari, M.A., Chaudhary, B.A., Uzair, M., Ashfaq, K. (2016). Cytotoxic, α-chymotrypsin and urease inhibition activities of the plant Heliotropium dasycarpum L. African Journal of Traditional, Complementary and Alternative Medicines, 13, 194-198.
  • Fu, P.P., Xia, Q., He, X., Barel, S., Edery, N., Beland, F.A., Shimshoni, J.A. (2017). Detection of Pyrrolizidine Alkaloid DNA Adducts in Livers of Cattle Poisoned with Heliotropium europaeum. Chemical Research in Toxicology, 30, 851-858.
  • Sargın, S.A., Akçiçek, E., Selvi, S. (2013). An ethnobotanical study of medicinal plants used by the local people of Alaşehir (Manisa) in Turkey. Journal of Ethnopharmacology, 150, 860-874.
  • Loscher, W. (2011). Critical review of current animal models of seizures and epilepsy used in the discovery and development of new antiepileptic drugs. Seizure, 20, 359-368.
  • Koyunoğlu, S., Arıhan, O., Sara, Y., Onur, R., Kır, S., Çalış, İ. (2012). Paeoniflorin Inhibits Maximal Electroshock- and PTZ-induced Convulsions In Mice. Hacettepe University Journal of the Faculty of Pharmacy, 32, 17-30.
  • Erkeç Ergül, Ö., Arıhan, O. (2015). Pentylenetetrazole Kindling Epilepsy Model. Pentilentetrazol Tutuşma Epilepsi Modeli. Epilepsi, 21, 6-12.
  • Gawande, D.Y., Druzhilovsky, D., Gupta, R.C., Poroikov, V., Goel, R.K. (2017). Anticonvulsant activity and acute neurotoxic profile of Achyranthes aspera Linn., Journal of Ethnopharmacology, 202, 97-102.
  • Rajput, M.A., Khan, R.A., Assad, T. (2017). Anti-epileptic activity of Nelumbo nucifera fruit. Metabolic Brain Disease, 32, 1883-1887.
  • Shimshoni, J.A, Mulder, P.P., Bouznach, A., Edery, N., Pasval, I., Barel, S., Abd-El Khaliq, M., Perl, S. (2015). Heliotropium europaeum poisoning in cattle and analysis of its pyrrolizidine alkaloid profile. Journal of Agricultural and Food Chemistry, 63, 1664-1672.
  • Owolabi, M.A., Oribayo, O.O., Ukpo, G.E., Mbaka, G.O., Akindehin, O.E. (2015). A 5-month toxicity study of the ethanol extract of the leaves of Heliotropium indicum in Sprague Dawley rats after oral administration. Nigerian Quarterly Journal of Hospital Medicine, 25(3), 184-192.
  • Eröksüz, Y., Eröksüz, H., Ozer, H., Canatan, H., Yaman, I., Cevik, A. (2001). Toxicity of dietary Heliotropium dolosum seed to broiler chickens. Veterinary and Human Toxicology, 43, 334-338.
  • Eröksüz, Y., Eröksüz, H., Ozer, H., Sener, B., Tosun, F, Akyüz C. (2001). Toxicity of dietary Heliotropium dolosum seed to mice. Veterinary and Human Toxicology, 43, 152-155.
  • Sivarajan, Y.V., Balachandran, L. (1994). Ayurvedic drugs and their plant resources, Oxford and IBH publishing Co. Ltd., New Delhi, p. 570.
  • Tiwari, V.K.N., Tiwari Singh, B.D. (2001). Comparative studies of cytokinins on in vitro propagation of Becopa monnieri. Plant Cell, Tissue and Organ Culture, 66, 9-16.
  • Behera, K.K., Manjari Mishra, N., Rout, G.R. (2008). Potential Ethnomedicinal Plants at Kaptipada Forest Range Orissa, India and Their Uses. Journal of Economic and Taxonomic Botany, 32 (Suppl.), 194-202.
  • Khan, H., Khan, M.A., Hussain, S., Gaffar, R., Ashraf, N. (2016). In vivo antinociceptive and anticonvulsant activity of extracts of Heliotropium strigosum. Toxicology and Industrial Health, 32, 860-865.

EFFECT OF HELIOTROPIUM HIRSUTISSIMUM, HELIOTROPIUM DOLOSUM AND HELIOTROPIUM LASIOCARPUM METHANOLIC EXTRACTS IN PENTYLENTETRAZOL INDUCED CONVULSIONS ON MICE

Yıl 2018, Cilt: 42 Sayı: 3, 13 - 19, 30.09.2018

Okan Arıhan Songul Karakaya Ceyda Sibel Kılıc Ozlem Ergul Erkec Mehmet Kara Hayri Duman

Öz

Objective: Epilepsy is a common neurological problem known since ancient times. Modern treatment of epilepsy includes treatment with drugs to avoid seizures. Search for new antiepileptic drugs continues due to the presence of resistant patients to drug treatment. Plants are important sources of new drug discovery for epilepsy. We aimed to evaluate anticonvulsive activity of methanolic extracts of Heliotropium (Scorpion herb, Herb for warts in Turkish) species namely Heliotropium hirsutissimum Grauer, H. dolosum De Not and H. lasiocarpum Fisch. et Mey. on mice.

Material and Method: PTZ (pentylentetrazol) is used for the induction of convulsions. Experimental groups are PTZ and 3 groups of extracts of aforementioned Heliotropium species+PTZ.

Result and Discussion: In H. hirsutissimum and H. lasiocarpum groups, number of animals having tonic-clonic convulsions was decreased compared to PTZ and H. dolosum groups however difference was found insignificant. Latency time for first myoclonic convulsion and latency time for first tonic-clonic convulsion were prolonged in all Heliotropium groups compared to PTZ group (p>0.05). Number of ex animals following tonicclonic convulsions decreased in Heliotropium groups (p>0.05). Tonic-clonic convulsion period decreased in H. hirsutissimum and H. lasiocarpum (p>0.05). When results are evaluated together Heliotropium species may have an ethnopharmacological relevance for its anticonvulsive usage.

Anahtar Kelimeler

convulsion epilepsy Heliotropium PTZ pentylentetrazol

Kaynakça

  • Baranano, K., Hartman, A. (2008). The Ketogenic Diet: Uses in Epilepsy and Other Neurologic Illnesses. Current Treatment Options in Neurology, 10, 410-419.
  • Eadie, M.J. (2004). Could valerian have been the first anticonvulsant? Epilepsia, 45, 1338-1343.
  • Yapıcı, Ü., Hoşgören, H., Saya, Ö. (2009). Kurtalan (Siirt) İlçesinin Etnobotanik Özellikleri. Dicle Üniversitesi Ziya Gökalp Eğitim Fakültesi Dergisi, 12, 191-196.
  • Tuzlacı, E., Doğan, A. (2010). Turkish folk medicinal plants, IX: Ovacık (Tunceli). Marmara Pharmaceutical Journal, 14, 136-143.
  • Sarı, A.O., Oğuz, B., Bilgiç, A., Tort, N., Günseven. A., Şenol, S.G. (2010). Ege ve Güney Marmara Bölgelerinde Halk İlacı Olarak Kullanılan Bitkiler. Anadolu Journal of AARI, 20(2), 1-21.
  • Melikoğlu, R.G., Kurtoğlu, S. (2015). Türkiye’de Astım Tedavisinde Geleneksel Olarak Kullanılan Bitkiler. Marmara Pharmaceutical Journal, 19, 1-11.
  • Singh, B., Sharma, R.A. (2015). Anti-Inflammatory and Antimicrobial Properties of Flavonoids from Heliotropium subulatum Exudate. Inflammation & Allergy Drug Targets, 14(2), 125-132.
  • Khan, H., Khan, M.A., Gul, F., Hussain, S., Ashraf, N. (2015). Anti-inflammatory activity of Heliotropium strigosum in animal models. Toxicology and Industrial Health, 31, 1281-1287.
  • Janbaz, K.H., Javed, S., Saqib, F., Imran, I., Zia-Ul-Haq, M., De Feo, V. (2015). Validation of ethnopharmacological uses of Heliotropium strigosum Willd. as spasmolytic, bronchodilator and vasorelaxant remedy. BMC Complementary and Alternative Medicine, 15, 169.
  • Murugesh, K., Yeligar, V., Dash, D.K., Sengupta, P., Maiti, B.C., Maity, T.K. (2006). Antidiabetic, antioxidant and antihyperlipidemic status of Heliotropium zeylanicum extract on streptozotocin-induced diabetes in rats. Biological and Pharmaceutical Bulletin, 29, 2202-2205.
  • Ghaffari, M.A., Chaudhary, B.A., Uzair, M., Ashfaq, K. (2016). Cytotoxic, α-chymotrypsin and urease inhibition activities of the plant Heliotropium dasycarpum L. African Journal of Traditional, Complementary and Alternative Medicines, 13, 194-198.
  • Fu, P.P., Xia, Q., He, X., Barel, S., Edery, N., Beland, F.A., Shimshoni, J.A. (2017). Detection of Pyrrolizidine Alkaloid DNA Adducts in Livers of Cattle Poisoned with Heliotropium europaeum. Chemical Research in Toxicology, 30, 851-858.
  • Sargın, S.A., Akçiçek, E., Selvi, S. (2013). An ethnobotanical study of medicinal plants used by the local people of Alaşehir (Manisa) in Turkey. Journal of Ethnopharmacology, 150, 860-874.
  • Loscher, W. (2011). Critical review of current animal models of seizures and epilepsy used in the discovery and development of new antiepileptic drugs. Seizure, 20, 359-368.
  • Koyunoğlu, S., Arıhan, O., Sara, Y., Onur, R., Kır, S., Çalış, İ. (2012). Paeoniflorin Inhibits Maximal Electroshock- and PTZ-induced Convulsions In Mice. Hacettepe University Journal of the Faculty of Pharmacy, 32, 17-30.
  • Erkeç Ergül, Ö., Arıhan, O. (2015). Pentylenetetrazole Kindling Epilepsy Model. Pentilentetrazol Tutuşma Epilepsi Modeli. Epilepsi, 21, 6-12.
  • Gawande, D.Y., Druzhilovsky, D., Gupta, R.C., Poroikov, V., Goel, R.K. (2017). Anticonvulsant activity and acute neurotoxic profile of Achyranthes aspera Linn., Journal of Ethnopharmacology, 202, 97-102.
  • Rajput, M.A., Khan, R.A., Assad, T. (2017). Anti-epileptic activity of Nelumbo nucifera fruit. Metabolic Brain Disease, 32, 1883-1887.
  • Shimshoni, J.A, Mulder, P.P., Bouznach, A., Edery, N., Pasval, I., Barel, S., Abd-El Khaliq, M., Perl, S. (2015). Heliotropium europaeum poisoning in cattle and analysis of its pyrrolizidine alkaloid profile. Journal of Agricultural and Food Chemistry, 63, 1664-1672.
  • Owolabi, M.A., Oribayo, O.O., Ukpo, G.E., Mbaka, G.O., Akindehin, O.E. (2015). A 5-month toxicity study of the ethanol extract of the leaves of Heliotropium indicum in Sprague Dawley rats after oral administration. Nigerian Quarterly Journal of Hospital Medicine, 25(3), 184-192.
  • Eröksüz, Y., Eröksüz, H., Ozer, H., Canatan, H., Yaman, I., Cevik, A. (2001). Toxicity of dietary Heliotropium dolosum seed to broiler chickens. Veterinary and Human Toxicology, 43, 334-338.
  • Eröksüz, Y., Eröksüz, H., Ozer, H., Sener, B., Tosun, F, Akyüz C. (2001). Toxicity of dietary Heliotropium dolosum seed to mice. Veterinary and Human Toxicology, 43, 152-155.
  • Sivarajan, Y.V., Balachandran, L. (1994). Ayurvedic drugs and their plant resources, Oxford and IBH publishing Co. Ltd., New Delhi, p. 570.
  • Tiwari, V.K.N., Tiwari Singh, B.D. (2001). Comparative studies of cytokinins on in vitro propagation of Becopa monnieri. Plant Cell, Tissue and Organ Culture, 66, 9-16.
  • Behera, K.K., Manjari Mishra, N., Rout, G.R. (2008). Potential Ethnomedicinal Plants at Kaptipada Forest Range Orissa, India and Their Uses. Journal of Economic and Taxonomic Botany, 32 (Suppl.), 194-202.
  • Khan, H., Khan, M.A., Hussain, S., Gaffar, R., Ashraf, N. (2016). In vivo antinociceptive and anticonvulsant activity of extracts of Heliotropium strigosum. Toxicology and Industrial Health, 32, 860-865.
Toplam 26 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Araştırma Makalesi
Yazarlar

Okan Arıhan

Songul Karakaya Bu kişi benim

Ceyda Sibel Kılıc Bu kişi benim

Ozlem Ergul Erkec Bu kişi benim

Mehmet Kara Bu kişi benim

Hayri Duman Bu kişi benim

Yayımlanma Tarihi 30 Eylül 2018
Gönderilme Tarihi 10 Mayıs 2018
Yayımlandığı Sayı Yıl 2018 Cilt: 42 Sayı: 3

Kaynak Göster

APA Arıhan, O., Karakaya, S., Kılıc, C. S., Ergul Erkec, O., vd. (2018). EFFECT OF HELIOTROPIUM HIRSUTISSIMUM, HELIOTROPIUM DOLOSUM AND HELIOTROPIUM LASIOCARPUM METHANOLIC EXTRACTS IN PENTYLENTETRAZOL INDUCED CONVULSIONS ON MICE. Journal of Faculty of Pharmacy of Ankara University, 42(3), 13-19.
AMA Arıhan O, Karakaya S, Kılıc CS, Ergul Erkec O, Kara M, Duman H. EFFECT OF HELIOTROPIUM HIRSUTISSIMUM, HELIOTROPIUM DOLOSUM AND HELIOTROPIUM LASIOCARPUM METHANOLIC EXTRACTS IN PENTYLENTETRAZOL INDUCED CONVULSIONS ON MICE. Ankara Ecz. Fak. Derg. Eylül 2018;42(3):13-19.
Chicago Arıhan, Okan, Songul Karakaya, Ceyda Sibel Kılıc, Ozlem Ergul Erkec, Mehmet Kara, ve Hayri Duman. “EFFECT OF HELIOTROPIUM HIRSUTISSIMUM, HELIOTROPIUM DOLOSUM AND HELIOTROPIUM LASIOCARPUM METHANOLIC EXTRACTS IN PENTYLENTETRAZOL INDUCED CONVULSIONS ON MICE”. Journal of Faculty of Pharmacy of Ankara University 42, sy. 3 (Eylül 2018): 13-19.
EndNote Arıhan O, Karakaya S, Kılıc CS, Ergul Erkec O, Kara M, Duman H (01 Eylül 2018) EFFECT OF HELIOTROPIUM HIRSUTISSIMUM, HELIOTROPIUM DOLOSUM AND HELIOTROPIUM LASIOCARPUM METHANOLIC EXTRACTS IN PENTYLENTETRAZOL INDUCED CONVULSIONS ON MICE. Journal of Faculty of Pharmacy of Ankara University 42 3 13–19.
IEEE O. Arıhan, S. Karakaya, C. S. Kılıc, O. Ergul Erkec, M. Kara, ve H. Duman, “EFFECT OF HELIOTROPIUM HIRSUTISSIMUM, HELIOTROPIUM DOLOSUM AND HELIOTROPIUM LASIOCARPUM METHANOLIC EXTRACTS IN PENTYLENTETRAZOL INDUCED CONVULSIONS ON MICE”, Ankara Ecz. Fak. Derg., c. 42, sy. 3, ss. 13–19, 2018.
ISNAD Arıhan, Okan vd. “EFFECT OF HELIOTROPIUM HIRSUTISSIMUM, HELIOTROPIUM DOLOSUM AND HELIOTROPIUM LASIOCARPUM METHANOLIC EXTRACTS IN PENTYLENTETRAZOL INDUCED CONVULSIONS ON MICE”. Journal of Faculty of Pharmacy of Ankara University 42/3 (Eylül 2018), 13-19.
JAMA Arıhan O, Karakaya S, Kılıc CS, Ergul Erkec O, Kara M, Duman H. EFFECT OF HELIOTROPIUM HIRSUTISSIMUM, HELIOTROPIUM DOLOSUM AND HELIOTROPIUM LASIOCARPUM METHANOLIC EXTRACTS IN PENTYLENTETRAZOL INDUCED CONVULSIONS ON MICE. Ankara Ecz. Fak. Derg. 2018;42:13–19.
MLA Arıhan, Okan vd. “EFFECT OF HELIOTROPIUM HIRSUTISSIMUM, HELIOTROPIUM DOLOSUM AND HELIOTROPIUM LASIOCARPUM METHANOLIC EXTRACTS IN PENTYLENTETRAZOL INDUCED CONVULSIONS ON MICE”. Journal of Faculty of Pharmacy of Ankara University, c. 42, sy. 3, 2018, ss. 13-19.
Vancouver Arıhan O, Karakaya S, Kılıc CS, Ergul Erkec O, Kara M, Duman H. EFFECT OF HELIOTROPIUM HIRSUTISSIMUM, HELIOTROPIUM DOLOSUM AND HELIOTROPIUM LASIOCARPUM METHANOLIC EXTRACTS IN PENTYLENTETRAZOL INDUCED CONVULSIONS ON MICE. Ankara Ecz. Fak. Derg. 2018;42(3):13-9.
 Kapak Resmi İndir

Kapsam ve Amaç

Ankara Üniversitesi Eczacılık Fakültesi Dergisi, açık erişim, hakemli bir dergi olup Türkçe veya İngilizce olarak farmasötik bilimler alanındaki önemli gelişmeleri içeren orijinal araştırmalar, derlemeler ve kısa bildiriler için uluslararası bir yayım ortamıdır. Bilimsel toplantılarda sunulan bildiriler supleman özel sayısı olarak dergide yayımlanabilir. Ayrıca, tüm farmasötik alandaki gelecek ve önceki ulusal ve uluslararası bilimsel toplantılar ile sosyal aktiviteleri içerir.