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Molecular detection of Ovine listeric abortion in Nineveh governorate, Iraq

Yıl 2024, Cilt: 19 Sayı: 1, 46 - 51, 26.04.2024

Hadeel Mohammad Maab Ibrahim Al-farwachi Balsam Rasheed

https://doi.org/10.17094/vetsci.1415509

Öz

Listeriosis is a important abortive diseases in sheep , and is considered one of the most risky bacterial zoonotic disease worldwide.The study was carried out in 50 sheep flocks placed in the Nineveh governorate, Iraq during November and December 2022, a total of 300 specimens of blood , abomasum content and brain ( 100 each)were collected from local breed ovine aborted fetues(in the last stage of gestation) were obtained to be tested for molecular detection of Listeria monocytogenes . L monocytogenes DNA was detected in a total of 61(20.3%) specimens , distributed as: 35(57.4%) ,15(24.6% and 11(18.0%) strains from fetal brain, abomasum and blood specimens respectively using direct genus-specific conventional polymerase chain reaction (prfA gene) C- PCR. Two L. monocytogenes strains ( HMB1 listeriolysin, HMB 2 listeriolysin ) deposited in GenBank under accession numbers LC769365.1,and LC769366.1. All L. monocytogenes strains were positive for three genes (InlJ, InlA, and hlyA) except actA gene was detected in 46 (75.4%) strains. In conclusion , L. monocytogenes is one of the important causal agents of abortion in sheep flocks in Nineveh province , Iraq, and most of the brain specimens were positive for listeric infection compared to the other specimens.

Anahtar Kelimeler

Listerosis Abortion Ovine Listeriolysin Iraq

Kaynakça

  • 1. Gebretensay A, Alemayehu G, Rekik M, et al. Risk factors for reproductive disorders and major infectious causes of abortion in sheep in the highlands of Ethiopia. Small Rum Res. 2019;177(1):1-9.
  • 2. Constable PD, Hinchcliff KW, Done SH, Grünberg W. Veterinary medicine: A textbook of the diseases of cattle, horses,sheep, pigs, and goats. 11th ed. Amsterdam: Elsevier Ltd.; 2017:2278.
  • 3. Schlech III WF. Epidemiology and clinical manifestations of Listeria monocytogenes infection. Microbiol Spectr. 2019; 7(3):3-7.
  • 4. Anderson ML. Infectious causes of bovine abortion during mid- to late-gestation. Theriogenol. 2007;68:474-486.
  • 5. Quereda JJ, Andersson C, Cossart P, et al. Role in virulence of phospholipases, listeriolysin O and listeriolysin S from epidemic Listeria monocytogenes using the chicken embryo infection model. Vet Res. 2018;49:13.
  • 6. Allerberger F. Listeria: growth, phenotypic differentiation and molecular microbiology. FEMS Immunol Med Microbiol. 2003;35(3):183-189.
  • 7. Lantz PG, Tjerneld F, Borch E, Hahn-Hagerdal B, Radstrom P. Enhanced sensitivity in PCR detection of Listeria monocytogenes in soft cheese through use of an aqueous two-phase system as a sample preparation method. J Appl Environ Microbiol. 1994;60(9):3416-3418.
  • 8. Somer L, Kash Y. A PCR method based on 16S rRNA sequence for simultaneous detection of the genus Listeria and the species Listeria monocytogenes in food products. J Food Prot. 2003;66(9):1658-1665.
  • 9. Al-Brefkania AMT, Narin A Rasheed NA, Hussein NR. Virulotyping of listeria monocytogenes isolated from human and food products samples using multiplex pcr in duhok province, Iraq. Sci J of the University of Zakho. 2023;11(2):215–219.
  • 10. Noomi BS, Anwar SHA, Salih SM. Detection of Listeria monocytogenes in raw milk and aborted cow cases at Salahudeen province. Iraqi J of Agri Sci. 2021;52(2):315-321.
  • 11. Jami S, Jamshidi A, Khanzadi S. The presence of Listeria monocytogenes in raw milk samples in Mashhad, Iran. Iran J Vet Med. 2010; 11(4):363-367.
  • 12. Skowron K, Wiktorczyk N, Grudlewska K, et al. Phenotypic and genotypic evaluation of Listeria monocytogenes strains isolated from fish and fish processing plants. Ann Microbiol. 2019;69:469-482.
  • 13. Liu D, Lawrence ML, Austin FW, Ainsworth AJ. A multiplex PCR for species-and virulence-specific determination of Listeria monocytogenes. J Microbial Methods. 2007;71(2):133-140.
  • 14. Hudson JA, Lake RJ, Savill MG, Scholes P, McCormick RE. Rapid detection of Listeria monocytogenes in ham samples using immunomagnetic separation followed by polymerase chain reaction. J Appl Microbial. 2001;90(4):614-621.
  • 15. Suárez M, González-Zorn B, Vega Y, Chico-Calero I, Vázquez-Boland JA. A role for ActA in epithelial cell invasion by Listeria monocytogenes. Cell Microbiol. 2001;3(12):853-64.
  • 16. Bubert A, Hein I, Rauch M, Lehner A, et al. Detection and differentiation of Listeria spp. by a single reaction based multiplex PCR. J Appl Environ Microbiol. 1999;65(10):4688-4892.
  • 17. Hatipoğlu F, Terzi F, Özdemir Ö, Ortatatli M, Çiftçi MK, Ateş MB. Comparison of histopathological, immunohistochemical and real-time PCR methods for diagnosis of listeriosis in ruminants with encephalitis. Kafkas Univ Vet Fak Derg. 2022;28(5):643-652.
  • 18. Haligur M, Aydogan A, Ozmen O, Ipek V. Immunohistochemical evaluation of natural cases of encephalitic listeriosis in sheep. Biotech Histochem. 2019;94(5):341-347.
  • 19. Janzten MM, Navas J, Corujo A, Moreno R, López V, Martínez-Suárez JV. Specific detection of Listeria monocytogenes in foods using commercial methods: From chromogenic media to real-time PCR. Span J Agric Res. 2006;4:235-247.
  • 20. Haley BJ, Sonnier J, Schukken YH, Karns JS, Van Kessel JA. Diversity of Listeria monocytogenes within a U.S. dairy herd, 2004–2010. Foodborne Pathog Dis. 2015;12:844-850.
  • 21. Yawoz M, Jaafar SE, Alali F, Babur C. Seroprevalence of camels listeriosis, brucellosis and toxoplasmosis from Kirkuk province, Iraq. Pak Vet J. 2021;41(3):335-340.
  • 22. Mohammad HA, AL-Farwachi MI, Rasheed BY. Seroprevalence of anti-listeriolysin O amongst aborted ewes in Nineveh province, Iraq. Iraqi J of Vet Sci. 2023;37(Supplement IV):227-231.
  • 23. Monteiro LR, Mesquita AJD, André MCD, et al. Molecular characterization of Listeria monocytogenes isolated from animal products in a city of Northern Brazil. Cienc Rural. 2013;43:1443-1448.
  • 24. Shoukat S, Mailk SVS, Rawool DB, et al. A Study on Detection of pathogenic Listeria monocytogenes in ovine’s of Kashmir region having abortion or history of abortion. Proc Natl Acad Sci India. Sect B Biol Sci. 2014;84: 311.
  • 25. Agerholm JS, Aalbaek B, Fog-Larsen AM, et al. Veterinary and medical aspects of abortion in Danish sheep. APMIS. 2006; 114: 146-152.
  • 26. El-Gedawy AA, Rasha A, MohsenA et al. Role of Listeria monocytogenes in abortion of ewes. Anim Healt Res J. 2019;7(4):979-987.
  • 27. Ezzat M, Ibrahim M, ELgedway A, et al. Prevalence, phenotypic characterization of listeria monocytogenes isolated from diseased sheep. SCVMJ. 2022;27(2):223-233.
  • 28. Wagner M, Melzner D, Bago Z, et al. Outbreak of clinical listeriosis in sheep: evaluation from possible contamination routes from feed to raw produce and humans. J Vet Med B Infect Dis Vet Public Health. 2005;52:278-283.
  • 29. Shakuntala I, Das S, Ghatak S, et al. Prevalence, characterization, and genetic diversity of Listeria monocytogenes isolated from foods of animal origin in North East India. Food Biotechnol. 2019;33:237-250.
  • 30. Laximan S, Kaur S, Aulakh RS, et al. Molecular characterization of Listeria monocytogenes in bovine milk and evaluating the sensitivity of PCR for direct detection in milk. Indian J Anim Sci. 2016;86:512-517.

Molecular detection of Ovine listeric abortion in Nineveh governorate, Iraq

Yıl 2024, Cilt: 19 Sayı: 1, 46 - 51, 26.04.2024

Hadeel Mohammad Maab Ibrahim Al-farwachi Balsam Rasheed

https://doi.org/10.17094/vetsci.1415509

Öz

Listeriosis is a important abortive diseases in sheep , and is considered one of the most risky bacterial zoonotic disease worldwide.The study was carried out in 50 sheep flocks placed in the Nineveh governorate, Iraq during November and December 2022, a total of 300 specimens of blood , abomasum content and brain ( 100 each)were collected from local breed ovine aborted fetues(in the last stage of gestation) were obtained to be tested for molecular detection of Listeria monocytogenes . L monocytogenes DNA was detected in a total of 61(20.3%) specimens , distributed as: 35(57.4%) ,15(24.6% and 11(18.0%) strains from fetal brain, abomasum and blood specimens respectively using direct genus-specific conventional polymerase chain reaction (prfA gene) C- PCR. Two L. monocytogenes strains ( HMB1 listeriolysin, HMB 2 listeriolysin ) deposited in GenBank under accession numbers LC769365.1,and LC769366.1. All L. monocytogenes strains were positive for three genes (InlJ, InlA, and hlyA) except actA gene was detected in 46 (75.4%) strains. In conclusion , L. monocytogenes is one of the important causal agents of abortion in sheep flocks in Nineveh province , Iraq, and most of the brain specimens were positive for listeric infection compared to the other specimens.

Anahtar Kelimeler

Keywords: Listerosis Abortion ovine listeriolysin Iraq.

Etik Beyan

The institutional care of animals and utilization authority of the Veterinary Medicine College, University of Mosul, acknowledged this research (authorization number UM.2022.032)

Destekleyen Kurum

This study results from a Ph.D. thesis Subsidized by the College of Veterinary Medicine, University of Mosul / Iraq.

Teşekkür

Thanks

Kaynakça

  • 1. Gebretensay A, Alemayehu G, Rekik M, et al. Risk factors for reproductive disorders and major infectious causes of abortion in sheep in the highlands of Ethiopia. Small Rum Res. 2019;177(1):1-9.
  • 2. Constable PD, Hinchcliff KW, Done SH, Grünberg W. Veterinary medicine: A textbook of the diseases of cattle, horses,sheep, pigs, and goats. 11th ed. Amsterdam: Elsevier Ltd.; 2017:2278.
  • 3. Schlech III WF. Epidemiology and clinical manifestations of Listeria monocytogenes infection. Microbiol Spectr. 2019; 7(3):3-7.
  • 4. Anderson ML. Infectious causes of bovine abortion during mid- to late-gestation. Theriogenol. 2007;68:474-486.
  • 5. Quereda JJ, Andersson C, Cossart P, et al. Role in virulence of phospholipases, listeriolysin O and listeriolysin S from epidemic Listeria monocytogenes using the chicken embryo infection model. Vet Res. 2018;49:13.
  • 6. Allerberger F. Listeria: growth, phenotypic differentiation and molecular microbiology. FEMS Immunol Med Microbiol. 2003;35(3):183-189.
  • 7. Lantz PG, Tjerneld F, Borch E, Hahn-Hagerdal B, Radstrom P. Enhanced sensitivity in PCR detection of Listeria monocytogenes in soft cheese through use of an aqueous two-phase system as a sample preparation method. J Appl Environ Microbiol. 1994;60(9):3416-3418.
  • 8. Somer L, Kash Y. A PCR method based on 16S rRNA sequence for simultaneous detection of the genus Listeria and the species Listeria monocytogenes in food products. J Food Prot. 2003;66(9):1658-1665.
  • 9. Al-Brefkania AMT, Narin A Rasheed NA, Hussein NR. Virulotyping of listeria monocytogenes isolated from human and food products samples using multiplex pcr in duhok province, Iraq. Sci J of the University of Zakho. 2023;11(2):215–219.
  • 10. Noomi BS, Anwar SHA, Salih SM. Detection of Listeria monocytogenes in raw milk and aborted cow cases at Salahudeen province. Iraqi J of Agri Sci. 2021;52(2):315-321.
  • 11. Jami S, Jamshidi A, Khanzadi S. The presence of Listeria monocytogenes in raw milk samples in Mashhad, Iran. Iran J Vet Med. 2010; 11(4):363-367.
  • 12. Skowron K, Wiktorczyk N, Grudlewska K, et al. Phenotypic and genotypic evaluation of Listeria monocytogenes strains isolated from fish and fish processing plants. Ann Microbiol. 2019;69:469-482.
  • 13. Liu D, Lawrence ML, Austin FW, Ainsworth AJ. A multiplex PCR for species-and virulence-specific determination of Listeria monocytogenes. J Microbial Methods. 2007;71(2):133-140.
  • 14. Hudson JA, Lake RJ, Savill MG, Scholes P, McCormick RE. Rapid detection of Listeria monocytogenes in ham samples using immunomagnetic separation followed by polymerase chain reaction. J Appl Microbial. 2001;90(4):614-621.
  • 15. Suárez M, González-Zorn B, Vega Y, Chico-Calero I, Vázquez-Boland JA. A role for ActA in epithelial cell invasion by Listeria monocytogenes. Cell Microbiol. 2001;3(12):853-64.
  • 16. Bubert A, Hein I, Rauch M, Lehner A, et al. Detection and differentiation of Listeria spp. by a single reaction based multiplex PCR. J Appl Environ Microbiol. 1999;65(10):4688-4892.
  • 17. Hatipoğlu F, Terzi F, Özdemir Ö, Ortatatli M, Çiftçi MK, Ateş MB. Comparison of histopathological, immunohistochemical and real-time PCR methods for diagnosis of listeriosis in ruminants with encephalitis. Kafkas Univ Vet Fak Derg. 2022;28(5):643-652.
  • 18. Haligur M, Aydogan A, Ozmen O, Ipek V. Immunohistochemical evaluation of natural cases of encephalitic listeriosis in sheep. Biotech Histochem. 2019;94(5):341-347.
  • 19. Janzten MM, Navas J, Corujo A, Moreno R, López V, Martínez-Suárez JV. Specific detection of Listeria monocytogenes in foods using commercial methods: From chromogenic media to real-time PCR. Span J Agric Res. 2006;4:235-247.
  • 20. Haley BJ, Sonnier J, Schukken YH, Karns JS, Van Kessel JA. Diversity of Listeria monocytogenes within a U.S. dairy herd, 2004–2010. Foodborne Pathog Dis. 2015;12:844-850.
  • 21. Yawoz M, Jaafar SE, Alali F, Babur C. Seroprevalence of camels listeriosis, brucellosis and toxoplasmosis from Kirkuk province, Iraq. Pak Vet J. 2021;41(3):335-340.
  • 22. Mohammad HA, AL-Farwachi MI, Rasheed BY. Seroprevalence of anti-listeriolysin O amongst aborted ewes in Nineveh province, Iraq. Iraqi J of Vet Sci. 2023;37(Supplement IV):227-231.
  • 23. Monteiro LR, Mesquita AJD, André MCD, et al. Molecular characterization of Listeria monocytogenes isolated from animal products in a city of Northern Brazil. Cienc Rural. 2013;43:1443-1448.
  • 24. Shoukat S, Mailk SVS, Rawool DB, et al. A Study on Detection of pathogenic Listeria monocytogenes in ovine’s of Kashmir region having abortion or history of abortion. Proc Natl Acad Sci India. Sect B Biol Sci. 2014;84: 311.
  • 25. Agerholm JS, Aalbaek B, Fog-Larsen AM, et al. Veterinary and medical aspects of abortion in Danish sheep. APMIS. 2006; 114: 146-152.
  • 26. El-Gedawy AA, Rasha A, MohsenA et al. Role of Listeria monocytogenes in abortion of ewes. Anim Healt Res J. 2019;7(4):979-987.
  • 27. Ezzat M, Ibrahim M, ELgedway A, et al. Prevalence, phenotypic characterization of listeria monocytogenes isolated from diseased sheep. SCVMJ. 2022;27(2):223-233.
  • 28. Wagner M, Melzner D, Bago Z, et al. Outbreak of clinical listeriosis in sheep: evaluation from possible contamination routes from feed to raw produce and humans. J Vet Med B Infect Dis Vet Public Health. 2005;52:278-283.
  • 29. Shakuntala I, Das S, Ghatak S, et al. Prevalence, characterization, and genetic diversity of Listeria monocytogenes isolated from foods of animal origin in North East India. Food Biotechnol. 2019;33:237-250.
  • 30. Laximan S, Kaur S, Aulakh RS, et al. Molecular characterization of Listeria monocytogenes in bovine milk and evaluating the sensitivity of PCR for direct detection in milk. Indian J Anim Sci. 2016;86:512-517.
Toplam 30 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Tanı ve Teşhis
Bölüm Araştırma Makaleleri
Yazarlar

Hadeel Mohammad Bu kişi benim 0000-0003-3918-1761

Maab Ibrahim Al-farwachi 0000-0002-2288-6274

Balsam Rasheed 0000-0002-4557-250X

Yayımlanma Tarihi 26 Nisan 2024
Gönderilme Tarihi 5 Ocak 2024
Kabul Tarihi 27 Mart 2024
Yayımlandığı Sayı Yıl 2024 Cilt: 19 Sayı: 1

Kaynak Göster

APA Mohammad, H., Ibrahim Al-farwachi, M., & Rasheed, B. (2024). Molecular detection of Ovine listeric abortion in Nineveh governorate, Iraq. Veterinary Sciences and Practices, 19(1), 46-51. https://doi.org/10.17094/vetsci.1415509
AMA Mohammad H, Ibrahim Al-farwachi M, Rasheed B. Molecular detection of Ovine listeric abortion in Nineveh governorate, Iraq. Veterinary Sciences and Practices. Nisan 2024;19(1):46-51. doi:10.17094/vetsci.1415509
Chicago Mohammad, Hadeel, Maab Ibrahim Al-farwachi, ve Balsam Rasheed. “Molecular Detection of Ovine Listeric Abortion in Nineveh Governorate, Iraq”. Veterinary Sciences and Practices 19, sy. 1 (Nisan 2024): 46-51. https://doi.org/10.17094/vetsci.1415509.
EndNote Mohammad H, Ibrahim Al-farwachi M, Rasheed B (01 Nisan 2024) Molecular detection of Ovine listeric abortion in Nineveh governorate, Iraq. Veterinary Sciences and Practices 19 1 46–51.
IEEE H. Mohammad, M. Ibrahim Al-farwachi, ve B. Rasheed, “Molecular detection of Ovine listeric abortion in Nineveh governorate, Iraq”, Veterinary Sciences and Practices, c. 19, sy. 1, ss. 46–51, 2024, doi: 10.17094/vetsci.1415509.
ISNAD Mohammad, Hadeel vd. “Molecular Detection of Ovine Listeric Abortion in Nineveh Governorate, Iraq”. Veterinary Sciences and Practices 19/1 (Nisan 2024), 46-51. https://doi.org/10.17094/vetsci.1415509.
JAMA Mohammad H, Ibrahim Al-farwachi M, Rasheed B. Molecular detection of Ovine listeric abortion in Nineveh governorate, Iraq. Veterinary Sciences and Practices. 2024;19:46–51.
MLA Mohammad, Hadeel vd. “Molecular Detection of Ovine Listeric Abortion in Nineveh Governorate, Iraq”. Veterinary Sciences and Practices, c. 19, sy. 1, 2024, ss. 46-51, doi:10.17094/vetsci.1415509.
Vancouver Mohammad H, Ibrahim Al-farwachi M, Rasheed B. Molecular detection of Ovine listeric abortion in Nineveh governorate, Iraq. Veterinary Sciences and Practices. 2024;19(1):46-51.
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