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Fasciola Hepatıca İle Doğal Enfekte Sığırlarda Koagulasyon Profilleri

Year 2022, , 160 - 164, 30.12.2022

Erhan Yalçınkaya , Onur Basbug

https://doi.org/10.51754/cusbed.1093396

Abstract

Özet: Dünya gelinde yaygın olarak görülen Fasciola hepatica, koyun ve sığır başta olmak üzere bir çok hayvaın karaciğerini etkileyen ve zoonotik trematodtur. Bu çalışmada Fasciola hepatica tespit edilen sığırlarda koagülasyon profilinde meydana gelebilecek değişimlerin belirlenmesi amaçlanmıştır. Araştırmanın materyalini çiftliklerdeki 282 adet sığır oluşturdu. çalışma kapsamında , bu sığırların klinik ve labratuvar sonuçlarına göre; 20 tanesi hastalıklı gruba, 20 sığır ise sağlıklı gruba dahil edildi. Araştırma yapılacak hayvanların genel klinik muayenesi yapıldıktan sonra dışkı ve kan örnekleri alındı. Bu hayvanlardan alınan gaita numuneleri Fasciola spp. yumurtalarının belirlenmesine yönelik olarak, sedimentasyon-çinko sülfat flotasyon metodu kullanıldı. Ayrıca ELISA yöntemi ile serum örneklerinden Fasciola hepatica antikorları ile araştırıldı. Na-sitrat’lı tüplere alınan kan örneklerinden koagulasyon profilleri incelendi. Yapılan koagülasyon analizi sonucunda pozitif örneklerin parsiyel tromboplastin zamanı (aPTT), protrombin zamanı (PT) ve International Normalized Ratio (INR) 'de uzama meydana getirdiği tespit edildi. Sonuç olarak, kronik fasiolozisin sığırlarda hematolojik ve hemostatik parametrelerde önemli değişikliklere neden olabileceği kanısına varılmıştır.

Keywords

Fasciola hepatica Sığır Koagulasyon

References

  • Ahmed, A. S. H. (2014). Prevalence and Risk Factors of Sheep Paramphistomiasis, Fascioliasis and Schistosomiasis in the White Nile State, Sudan (Doctoral dissertation, Sudan University of Science and Technology).
  • Aksakal, M., & Özer, E. (1987). Akkaraman koyunlarında antelmentik ilaçlarla tedaviden önce ve sonra hematolojik değerler ve kan plazması vitamin E düzeyi üzerine araştırmalar. Ankara Üniv Vet Fak Derg, 34, 72-84.
  • Alvarez Rojas, C. A., Ansell, B. R., Hall, R. S., Gasser, R. B., Young, N. D., Jex, A. R., & Scheerlinck, J. P. Y. (2015). Transcriptional analysis identifies key genes involved in metabolism, fibrosis/tissue repair and the immune response against Fasciola hepatica in sheep liver. Parasites & vectors, 8(1), 1-14.
  • Bostelmann, S. C. W., Luz, E., THOMAZ-SOCCOL, V., & Cirio, S. M. (2000). Histopatologia comparativa em fígados de bovinos, bubalinos e ovinos infectados por Fasciola hepatica. Archives of Veterinary Science, 5(1).
  • Celep, A., Açıcı, M., Çetindağ, M., Coşkun, Ş. Z., & Gürsoy, S. (1990). Samsun yöresi sığırlarında helmintolojik araştırmalar. Etlik Veteriner Mikrobiyoloji Dergisi, 6(6), 117-130.
  • Chowdhury, N., & Tada, I. (1994). Helminths of domesticated animals in Indian subcontinent. Helminthology, 1, 73-120.
  • Duncan, E. M., Casey, C. R., Duncan, B. M., & Lloyd, J. V. (1994). Effect of concentration of trisodium citrate anticoagulant on calculation of the international normalised ratio and the international sensitivity index of thromboplastin. Thrombosis and haemostasis, 72(07), 084-088.
  • Geurden, T., Somers, R., Thanh, N. T. G., Vien, L. V., Nga, V. T., Giang, H. H., ... & Vercruysse, J. (2008). Parasitic infections in dairy cattle around Hanoi, northern Vietnam. Veterinary Parasitology, 153(3-4), 384-388.
  • Gül, Y. (2006). Geviş Getiren Hayvanların İç Hastalıkları (Sığır, Koyun-Keçi). II. Baskı. Medipres Matbaacılık Ltd. Şti., Malatya, 452, 454.
  • Hawkins, C. D. (1984). The use of haemoglobin, packed-cell volume and serum sorbitol dehydrogenase as indicators of the development of fascioliasis in sheep. Veterinary Parasitology, 15(2), 125-133.
  • Huang, C., Wang, Y., Li, X., Ren, L., Zhao, J., Hu, Y., ... & Cao, B. (2020). Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. The lancet, 395(10223), 497-506. Heuwieser, W., Biesel, M., & Grunert, E. (1989). Physiological coagulation profile of dairy cattle. Journal of Veterinary Medicine Series A, 36(1‐10), 24-31.
  • Jadaon, M. M. (2012). Aetiology of Venous Thrombosis. In Venous Thrombosis-Principles and Practice. IntechOpen.
  • Kaplan, M., & Başpınar, S. (2009). Elazığ'da son 5 yılda kesilen kasaplık hayvanlarda fasciolosis sıklığı ve ekonomik önemi. Fırat Tıp Dergisi, 14(1), 25-27.
  • Karakoyun, F. D. İ. İ. (2016). Dondurulmuş Plazmada Protrombin Zamanı ve Aktive Parsiyel Tromboplastin Zamanı Stabilitesi.
  • Lip, G. Y., & Lowe, G. D. (1995). Fibrin D-dimer: a useful clinical marker of thrombogenesis?. Clinical Science, 89(3), 205-214.
  • Lisman, T., Porte, R. J., Leebeek, F. W., & Caldwell, S. H. (2006). Methodological issues with coagulation testing in patients with liver disease. Journal of thrombosis and haemostasis: JTH, 4(9), 2061-2062.
  • MacLeod, J. B., Lynn, M., McKenney, M. G., Cohn, S. M., & Murtha, M. (2003). Early coagulopathy predicts mortality in trauma. Journal of Trauma and Acute Care Surgery, 55(1), 39-44.
  • Mahdi, N. K., & Al-Baldawi, F. A. K. (1987). Hepatic fascioliasis in the abattoirs of Basrah. Annals of Tropical Medicine & Parasitology, 81(4), 377-379.
  • Mannucci, P. M. (2006). Abnormal hemostasis tests and bleeding in chronic liver disease: are they related? No. Journal of Thrombosis and Haemostasis, 4(4), 721-723.
  • Marcos, L. A., Yi, P., Machicado, A., Andrade, R., Samalvides, F., Sánchez, J., & Terashima, A. (2007). Hepatic fibrosis and Fasciola hepatica infection in cattle. Journal of helminthology, 81(4), 381-386.
  • Morris, D. D. (2002). Alterations in the clotting profile. Large Animal Internal Medicine, 2nd edition. New York: Mosby, 434-439.
  • Omran, S. A., Amin, H. M., El-Bassiouni, N. E., Essawy, F. M., & Toiema, S. M. (1994). Vitamin K dependent coagulation proteins in endemic hepatosplenomegaly in Egypt. Journal of clinical pathology, 47(6), 502-504.
  • Omran, S. A., Toiema, S. M., & El-Din, A. S. (1990). In vitro effect of Schistosoma worm antigen on some haemostatic parameters in hepatosplenic schistosomiasis. Egyptian Journal of Bilharziasis, 12(1-2), 17-34.
  • Osuna, O., Edds, G. T., & Blankespoor, H. D. (1977). Toxic effects of aflatoxin B1 in male Holstein calves with prior infection by flukes (Fasciola hepatica). American Journal of Veterinary Research, 38(3), 341-349.
  • Peterson, P., & Gottfried, E. L. (1982). The effects of inaccurate blood sample volume on prothrombin time (PT) and activated partial thromboplastin time (aPTT). Thrombosis and haemostasis, 47(02), 101-103.
  • Premkumar, M., & Sarin, S. K. (2020). Current Concepts in Coagulation Profile in Cirrhosis and Acute‐on‐Chronic Liver Failure. Clinical liver disease, 16(4), 158.
  • Riley, R. S., Rowe, D., & Fisher, L. M. (2000). Clinical utilization of the international normalized ratio (INR). Journal of clinical laboratory analysis, 14(3), 101-114.
  • Soulsby, E. J. L. (1968). Helminths, arthropods and protozoa of domesticated animals (of MSnnig's Veterinary helminthology and entomology). Helminths, arthropods and protozoa of domesticated animals (sixth edition of MSnnig's Veterinary helminthology and entomology).
  • Tang, N., Li, D., Wang, X., & Sun, Z. (2020). Abnormal coagulation parameters are associated with poor prognosis in patients with novel coronavirus pneumonia. Journal of thrombosis and haemostasis, 18(4), 844-847.
  • Toparlak, M. (1988). Van İli Belediye Mezbahasında Kesilen Koyunlarda Karaciğer Trematod Enfeksiyonları Üzerinde Araştırmalar. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 35(02.03), 270-274
  • Yavuz, A., İnci, A., Yıldırım, A., İça, A., & Düzlü, Ö. (2007). Sığırlarda Fasciola hepatica'nın yayılışı. Sağlık Bilimleri Dergisi, 16(2), 96-102.
  • Yildirim, A., Ica, A., Duzlu, Ö. & Inci, A. (2007). Prevalence and risk factors associated with Fasciola hepatica in cattle from Kayseri province, Turkey. Revue de médecine vétérinaire, 158.

Coagulation Profiles in Naturally Infective Cattle With Fasciola Hepatica

Year 2022, , 160 - 164, 30.12.2022

Erhan Yalçınkaya , Onur Basbug

https://doi.org/10.51754/cusbed.1093396

Abstract

: Fasciola hepatica, which is common in the world, is a zoonotic trematode that affects the liver of many animals, especially sheep and cattle. In this study, it was aimed to determine the changes that may occur in the coagulation profile in cattle with Fasciola hepatica detected. The material of the study consisted of 282 cattle on farms. Within the scope of the study, according to the clinical and laboratory results of these cattle; 20 cattle were included in the diseased group and 20 cattle were included in the healthy group. After the general clinical examination of the animals to be studied, stool and blood samples were taken. Stool samples taken from these animals Fasciola spp. Sedimentation-zinc sulfate flotation method was used to determine the eggs. In addition, Fasciola hepatica antibodies from serum samples were investigated by ELISA method. Coagulation profiles were examined from blood samples taken into tubes with Na-citrate. As a result of the coagulation analysis, it was determined that positive samples caused prolongation in activated partial thromboplastin time (aPTT), Prothrombin time (PT) and INR (International Normalized Ratio) . In conclusion, it was concluded that chronic fasciolosis may cause significant changes in hematological and hemostatic parameters in cattle.

Keywords

Fasciola hepatica Cattle Coagulation

References

  • Ahmed, A. S. H. (2014). Prevalence and Risk Factors of Sheep Paramphistomiasis, Fascioliasis and Schistosomiasis in the White Nile State, Sudan (Doctoral dissertation, Sudan University of Science and Technology).
  • Aksakal, M., & Özer, E. (1987). Akkaraman koyunlarında antelmentik ilaçlarla tedaviden önce ve sonra hematolojik değerler ve kan plazması vitamin E düzeyi üzerine araştırmalar. Ankara Üniv Vet Fak Derg, 34, 72-84.
  • Alvarez Rojas, C. A., Ansell, B. R., Hall, R. S., Gasser, R. B., Young, N. D., Jex, A. R., & Scheerlinck, J. P. Y. (2015). Transcriptional analysis identifies key genes involved in metabolism, fibrosis/tissue repair and the immune response against Fasciola hepatica in sheep liver. Parasites & vectors, 8(1), 1-14.
  • Bostelmann, S. C. W., Luz, E., THOMAZ-SOCCOL, V., & Cirio, S. M. (2000). Histopatologia comparativa em fígados de bovinos, bubalinos e ovinos infectados por Fasciola hepatica. Archives of Veterinary Science, 5(1).
  • Celep, A., Açıcı, M., Çetindağ, M., Coşkun, Ş. Z., & Gürsoy, S. (1990). Samsun yöresi sığırlarında helmintolojik araştırmalar. Etlik Veteriner Mikrobiyoloji Dergisi, 6(6), 117-130.
  • Chowdhury, N., & Tada, I. (1994). Helminths of domesticated animals in Indian subcontinent. Helminthology, 1, 73-120.
  • Duncan, E. M., Casey, C. R., Duncan, B. M., & Lloyd, J. V. (1994). Effect of concentration of trisodium citrate anticoagulant on calculation of the international normalised ratio and the international sensitivity index of thromboplastin. Thrombosis and haemostasis, 72(07), 084-088.
  • Geurden, T., Somers, R., Thanh, N. T. G., Vien, L. V., Nga, V. T., Giang, H. H., ... & Vercruysse, J. (2008). Parasitic infections in dairy cattle around Hanoi, northern Vietnam. Veterinary Parasitology, 153(3-4), 384-388.
  • Gül, Y. (2006). Geviş Getiren Hayvanların İç Hastalıkları (Sığır, Koyun-Keçi). II. Baskı. Medipres Matbaacılık Ltd. Şti., Malatya, 452, 454.
  • Hawkins, C. D. (1984). The use of haemoglobin, packed-cell volume and serum sorbitol dehydrogenase as indicators of the development of fascioliasis in sheep. Veterinary Parasitology, 15(2), 125-133.
  • Huang, C., Wang, Y., Li, X., Ren, L., Zhao, J., Hu, Y., ... & Cao, B. (2020). Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. The lancet, 395(10223), 497-506. Heuwieser, W., Biesel, M., & Grunert, E. (1989). Physiological coagulation profile of dairy cattle. Journal of Veterinary Medicine Series A, 36(1‐10), 24-31.
  • Jadaon, M. M. (2012). Aetiology of Venous Thrombosis. In Venous Thrombosis-Principles and Practice. IntechOpen.
  • Kaplan, M., & Başpınar, S. (2009). Elazığ'da son 5 yılda kesilen kasaplık hayvanlarda fasciolosis sıklığı ve ekonomik önemi. Fırat Tıp Dergisi, 14(1), 25-27.
  • Karakoyun, F. D. İ. İ. (2016). Dondurulmuş Plazmada Protrombin Zamanı ve Aktive Parsiyel Tromboplastin Zamanı Stabilitesi.
  • Lip, G. Y., & Lowe, G. D. (1995). Fibrin D-dimer: a useful clinical marker of thrombogenesis?. Clinical Science, 89(3), 205-214.
  • Lisman, T., Porte, R. J., Leebeek, F. W., & Caldwell, S. H. (2006). Methodological issues with coagulation testing in patients with liver disease. Journal of thrombosis and haemostasis: JTH, 4(9), 2061-2062.
  • MacLeod, J. B., Lynn, M., McKenney, M. G., Cohn, S. M., & Murtha, M. (2003). Early coagulopathy predicts mortality in trauma. Journal of Trauma and Acute Care Surgery, 55(1), 39-44.
  • Mahdi, N. K., & Al-Baldawi, F. A. K. (1987). Hepatic fascioliasis in the abattoirs of Basrah. Annals of Tropical Medicine & Parasitology, 81(4), 377-379.
  • Mannucci, P. M. (2006). Abnormal hemostasis tests and bleeding in chronic liver disease: are they related? No. Journal of Thrombosis and Haemostasis, 4(4), 721-723.
  • Marcos, L. A., Yi, P., Machicado, A., Andrade, R., Samalvides, F., Sánchez, J., & Terashima, A. (2007). Hepatic fibrosis and Fasciola hepatica infection in cattle. Journal of helminthology, 81(4), 381-386.
  • Morris, D. D. (2002). Alterations in the clotting profile. Large Animal Internal Medicine, 2nd edition. New York: Mosby, 434-439.
  • Omran, S. A., Amin, H. M., El-Bassiouni, N. E., Essawy, F. M., & Toiema, S. M. (1994). Vitamin K dependent coagulation proteins in endemic hepatosplenomegaly in Egypt. Journal of clinical pathology, 47(6), 502-504.
  • Omran, S. A., Toiema, S. M., & El-Din, A. S. (1990). In vitro effect of Schistosoma worm antigen on some haemostatic parameters in hepatosplenic schistosomiasis. Egyptian Journal of Bilharziasis, 12(1-2), 17-34.
  • Osuna, O., Edds, G. T., & Blankespoor, H. D. (1977). Toxic effects of aflatoxin B1 in male Holstein calves with prior infection by flukes (Fasciola hepatica). American Journal of Veterinary Research, 38(3), 341-349.
  • Peterson, P., & Gottfried, E. L. (1982). The effects of inaccurate blood sample volume on prothrombin time (PT) and activated partial thromboplastin time (aPTT). Thrombosis and haemostasis, 47(02), 101-103.
  • Premkumar, M., & Sarin, S. K. (2020). Current Concepts in Coagulation Profile in Cirrhosis and Acute‐on‐Chronic Liver Failure. Clinical liver disease, 16(4), 158.
  • Riley, R. S., Rowe, D., & Fisher, L. M. (2000). Clinical utilization of the international normalized ratio (INR). Journal of clinical laboratory analysis, 14(3), 101-114.
  • Soulsby, E. J. L. (1968). Helminths, arthropods and protozoa of domesticated animals (of MSnnig's Veterinary helminthology and entomology). Helminths, arthropods and protozoa of domesticated animals (sixth edition of MSnnig's Veterinary helminthology and entomology).
  • Tang, N., Li, D., Wang, X., & Sun, Z. (2020). Abnormal coagulation parameters are associated with poor prognosis in patients with novel coronavirus pneumonia. Journal of thrombosis and haemostasis, 18(4), 844-847.
  • Toparlak, M. (1988). Van İli Belediye Mezbahasında Kesilen Koyunlarda Karaciğer Trematod Enfeksiyonları Üzerinde Araştırmalar. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 35(02.03), 270-274
  • Yavuz, A., İnci, A., Yıldırım, A., İça, A., & Düzlü, Ö. (2007). Sığırlarda Fasciola hepatica'nın yayılışı. Sağlık Bilimleri Dergisi, 16(2), 96-102.
  • Yildirim, A., Ica, A., Duzlu, Ö. & Inci, A. (2007). Prevalence and risk factors associated with Fasciola hepatica in cattle from Kayseri province, Turkey. Revue de médecine vétérinaire, 158.
There are 32 citations in total.

Details

Primary Language Turkish
Subjects Veterinary Surgery
Journal Section Research Article
Authors

Erhan Yalçınkaya 0000-0002-7042-9115

Onur Basbug 0000-0003-3136-0589

Publication Date December 30, 2022
Published in Issue Year 2022

Cite

APA Yalçınkaya, E., & Basbug, O. (2022). Fasciola Hepatıca İle Doğal Enfekte Sığırlarda Koagulasyon Profilleri. Instıtute of Health Sciences Journal, 7(3), 160-164. https://doi.org/10.51754/cusbed.1093396

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