Abstract
Bu çalışma komplikasyonlu gebe ineklerin gebelik dönemlerinde serum haptoglobin düzeylerinin belirlenmesi amacıyla yapılmıştır. Çalışmaya aynı işletmeye ait doğum süreçleri takip edilen, sağlıklı doğum gerçekleştiren 14 inek ve komplikasyonlu gebeliğe sahip olan 14 inek olmak üzere toplam 28 hayvan dahil edilmiştir. Kan örnekleri gebeliklerinin 6. ayında vena jugularisten sarı kapaklı biyokimya tüplerine alınmıştır. Serum albümin, globulin ve total protein ölçümleri otoanalizör cihazı ile yapılmıştır. Haptoglobin seviyelerinin belirlenmesinde ticari test kiti kullanarak ELISA cihazında ölçülmüştür. Serum haptoglobin seviyeleri komplikasyonlu gebelerde 226.64±24.09 µg/ml; sağlıklı gebelerde ise 46.71±2.98 µg/ml olarak tespit edilmiştir. Komplikasyonlu ve sağlıklı gebelerde sırasıyla total protein 8,52±0,28-7,58±0,12 g/dL; albümin 3,31±0,08-3,34±0,04 g/dL; globulin 5,21±0,24-4,25±0,15 g/dL; alb/glb 0,65±0,03-0,80±0,04 olarak bulunmuştur. Serum haptoglobin, total protein, globulin ve alb/glb değerleri kontrol grubuna göre komplikasyonlu gebelik grubunda istatiksel olarak anlamlı görülmüştür (p<0.05).
References
- Alaçam, E. (2010). Evcil Hayvanlarda Doğum ve İnfertilite 7. Baskı. Medisan Yayınevi, Ankara.
- Aziz, D. M., & Mohammed, S. H. (2016). Alteration of serum haptoglobin concentration in normal parturition and dystocia affected cows. Iraqi Journal of Veterinary Sciences, 30(1), 19-22.
- Bayyit, E., & Merhan, O. (2020). Normal ve güç doğum yapan ineklerde bazı akut faz proteinlerinin ve oksidatif stres düzeyinin belirlenmesi. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 15(2), 145-150.
- Bhat, S. V., Anisha, J. P., Shynu, M., Jayavardhanan, K. K., & Ramnath, V. (2020). Higher concentration of haptoglogin indicates transient inflammation and negative energy balance in transition cows. Journal of Veterinary and Animal Sciences.
- Chan, J. P. W., Chu, C. C., Fung, H. P., Te Chuang, S., Lin, Y. C., Chu, R. M., & Lee, S. L. (2004). Serum haptoglobin concentration in cattle. Journal of Veterinary Medical Science, 66(1), 43-46.
- Dematawena, C. M. B., & Berger, P. J. (1997). Effect of dystocia on yield, fertility, and cow losses and an economic evaluation of dystocia scores for Holsteins. Journal of Dairy Science, 80(4), 754-761.
- Gädicke, P., Letelier, R., Chihuailaf, R., & RuizA, J. T. (2021). Variation in the Multivariate Relationships Between Serum Amyloid, Haptoglobin and Biochemical Profiles of Aborted and Non-Aborted Cows. SOJ Dairy Vet Sci, 1(1), 1-6.
- Hossein-Zadeh, N. G. (2013). Effects of main reproductive and health problems on the performance of dairy cows: a review. Spanish Journal of Agricultural Research, (3), 718-735.
- Keshavarzi, H., Sadeghi-Sefidmazgi, A., Ghorbani, G. R., Kowsar, R., Razmkabir, M., & Amer, P. (2020). Effect of abortion on milk production, health, and reproductive performance of Holstein dairy cattle. Animal reproduction science, 217, 106458.
- Lombard, J. E., Garry, F. B., Tomlinson, S. M., & Garber, L. P. (2007). Impacts of dystocia on health and survival of dairy calves. Journal of dairy science, 90(4), 1751-1760.
- Macmillan, K., Gobikrushanth, M., Helguera, I. L., Behrouzi, A., & Colazo, M. G. (2020). Relationships between early postpartum nutritional and metabolic profiles and subsequent reproductive performance of lactating dairy cows. Theriogenology, 151, 52-57.
- Morimatsu, M., Syuto, B., Shimada, N., Fujinaga, T., Yamamoto, S., Saito, M., & Naiki, M. (1991). Isolation and characterization of bovine haptoglobin from acute phase sera. Journal of Biological Chemistry, 266(18), 11833-11837.
- Oakes D.E., Parkinson T.J., England G.C.W. (2001). Arthur’s Veterinary Reproduction and Obstetrics, Eighth edition. Ed.by. Part three: Dystocia and other disorders associated with parturation. W.B. Saunders, London, ISBN, 978 0 7010 2\ \ 6 I.
- Pohl, A., Burfeind, O., & Heuwieser, W. (2015). The associations between postpartum serum haptoglobin concentration and metabolic status, calving difficulties, retained fetal membranes, and metritis. Journal of dairy science, 98(7), 4544-4551.
- SPSS (2014). IBM SPSS Statistics for Windows, version 22.00. SPSS Inc., Chicago, IL.
- Taşçene, N. (2017). Akut Faz Proteinlerinin Hayvanlarda Önemi. Lalahan Hayvancılık Araştırma Enstitüsü Dergisi, 57(1), 52-60.
Abstract
This study was carried out to determine serum haptoglobin levels of pregnant cows with complications during pregnancy. A total of 28 animals were included in the study, 14 cows with healthy birth and 14 cows with complicated pregnancies, whose birth processes were followed in the same farm. Blood samples were taken from the vena jugularis into yellow capped biochemistry tubes at the 6th month of their pregnancy. Serum albumin, globulin and total protein measurements were made with an autoanalyzer. Determination of haptoglobin levels was measured in an ELISA device using a commercial test kit. Serum haptoglobin levels were found as 226.64±24.09 µg/ml in pregnant cows with complications and 46.71±2.98 µg/ml in healthy pregnant cows. Total protein 8.52±0.28-7.58±0.12 g/dL, respectively; albumin 3.31±0.08-3.34±0.04 g/dL; globulin 5.21±0.24-4.25±0.15 g/dL; alb/glb 0.65±0.03-0.80±0.04 was found to be in complicated and healthy pregnant. Serum haptoglobin, total protein, globulin and alb/glb values were statistically significant in the complicated pregnancy group compared to the control group (p<0.05).
Keywords
References
- Alaçam, E. (2010). Evcil Hayvanlarda Doğum ve İnfertilite 7. Baskı. Medisan Yayınevi, Ankara.
- Aziz, D. M., & Mohammed, S. H. (2016). Alteration of serum haptoglobin concentration in normal parturition and dystocia affected cows. Iraqi Journal of Veterinary Sciences, 30(1), 19-22.
- Bayyit, E., & Merhan, O. (2020). Normal ve güç doğum yapan ineklerde bazı akut faz proteinlerinin ve oksidatif stres düzeyinin belirlenmesi. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 15(2), 145-150.
- Bhat, S. V., Anisha, J. P., Shynu, M., Jayavardhanan, K. K., & Ramnath, V. (2020). Higher concentration of haptoglogin indicates transient inflammation and negative energy balance in transition cows. Journal of Veterinary and Animal Sciences.
- Chan, J. P. W., Chu, C. C., Fung, H. P., Te Chuang, S., Lin, Y. C., Chu, R. M., & Lee, S. L. (2004). Serum haptoglobin concentration in cattle. Journal of Veterinary Medical Science, 66(1), 43-46.
- Dematawena, C. M. B., & Berger, P. J. (1997). Effect of dystocia on yield, fertility, and cow losses and an economic evaluation of dystocia scores for Holsteins. Journal of Dairy Science, 80(4), 754-761.
- Gädicke, P., Letelier, R., Chihuailaf, R., & RuizA, J. T. (2021). Variation in the Multivariate Relationships Between Serum Amyloid, Haptoglobin and Biochemical Profiles of Aborted and Non-Aborted Cows. SOJ Dairy Vet Sci, 1(1), 1-6.
- Hossein-Zadeh, N. G. (2013). Effects of main reproductive and health problems on the performance of dairy cows: a review. Spanish Journal of Agricultural Research, (3), 718-735.
- Keshavarzi, H., Sadeghi-Sefidmazgi, A., Ghorbani, G. R., Kowsar, R., Razmkabir, M., & Amer, P. (2020). Effect of abortion on milk production, health, and reproductive performance of Holstein dairy cattle. Animal reproduction science, 217, 106458.
- Lombard, J. E., Garry, F. B., Tomlinson, S. M., & Garber, L. P. (2007). Impacts of dystocia on health and survival of dairy calves. Journal of dairy science, 90(4), 1751-1760.
- Macmillan, K., Gobikrushanth, M., Helguera, I. L., Behrouzi, A., & Colazo, M. G. (2020). Relationships between early postpartum nutritional and metabolic profiles and subsequent reproductive performance of lactating dairy cows. Theriogenology, 151, 52-57.
- Morimatsu, M., Syuto, B., Shimada, N., Fujinaga, T., Yamamoto, S., Saito, M., & Naiki, M. (1991). Isolation and characterization of bovine haptoglobin from acute phase sera. Journal of Biological Chemistry, 266(18), 11833-11837.
- Oakes D.E., Parkinson T.J., England G.C.W. (2001). Arthur’s Veterinary Reproduction and Obstetrics, Eighth edition. Ed.by. Part three: Dystocia and other disorders associated with parturation. W.B. Saunders, London, ISBN, 978 0 7010 2\ \ 6 I.
- Pohl, A., Burfeind, O., & Heuwieser, W. (2015). The associations between postpartum serum haptoglobin concentration and metabolic status, calving difficulties, retained fetal membranes, and metritis. Journal of dairy science, 98(7), 4544-4551.
- SPSS (2014). IBM SPSS Statistics for Windows, version 22.00. SPSS Inc., Chicago, IL.
- Taşçene, N. (2017). Akut Faz Proteinlerinin Hayvanlarda Önemi. Lalahan Hayvancılık Araştırma Enstitüsü Dergisi, 57(1), 52-60.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Research Article |
| Authors | |
| Publication Date | December 30, 2022 |
| Published in Issue | Year 2022 |
