Research Article
Sıçanlarda Allium Schoenoprasum L. Etanol Ekstraktı Ve Akrilamid Uygulandıktan Sonra Hematolojik Parametrelerin Değerlendirilmesi
Abstract
Bu çalışmada, Allium schoenoprasum L. bitkisinin akrilamid toksisitesine karşı koruyucu etkisinin araştırılması amaçlanmıştır. Hayvan materyali olarak 32 adet Wistar-Albino dişi rat 4 gruba ayrıldı. Birinci grup kontrol grubunu oluşturdu. İkinci gruba 15 gün boyunca her gün 25 mg/kg Akrilamid gastrik sonda ile verildi. Üçüncü gruba 200 mg/kg Allium schoenoprasum L. etanol ekstresi gastrik sonda ile 15 gün verildi. Dördüncü gruba 25 mg/kg Acrylamide + 200 mg/kg Allium schoenoprasum L. etanol ekstresi gastrik sonda ile 15 gün verildi. Analiz sonucunda akrilamid grubundaki sıçanlarda RBC, HCT, HGB, MCV, MCH, MCHC WBC, Lenfosit, Monosit ve Eozinofil Lenfosit (%) seviyeleri düşmesine rağmen, nötrofil ve RDW miktarı arttı (P <0,05). Allium schoenoprasum L. bitkisinin akrilamit maruziyetinin olumsuz etkilerini düzeltmede etkili olabileceği belirlendi.
References
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- Ali MA, Aly EM, Elawady AI. (2014). Effectiveness of selenium on acrylamide toxicity to retina.Int J Opthalmol, 7(4):614-620
- Altinoz, E., Turkoz, Y. (2014). The protective role of N-Acetylcysteine against acrylamide-ınduced genotoxicity and oxidati ve stress ın rats. Gene Ther Mol Biol , 16: 35-43.
- Barber, D.S., Hunt, J.R., Ehrich, M.F., Lehning, E.J., LoPachin, R.M. (2001). Toxicokinetics and hemoglobin adduct formation in rats following subacute and subchronic acrylamide dosing. Metabolism, 22(3):341-353.
- Boettcher, M. I., Schettgen, T., Kutting, B., Pischetsrieder, M., Angerer, J. (2005). Mercapturic acids of acrylamide and glycidamide as biomarkers of the internal exposure to acrylamide in the general population. Mutat Res, 580: 167–76.
- Ceylan, S., Cetin, S., Camadan, Y., Saral, O., Ozsen, O., Tutus, A. (2019). Antibacterial and antioxidant activities of traditional medicinal plants from the Erzurum region of Turkey. Irish Journal of Medical Science,
- Claus, A., Carle, R., Schieber, A. (2008). Acrylamide in cereal products. A review Journal of Cereal Science, 47: 118-133.
- Comba, A., Oto, G., Comba, B., Ozdemir, H., Keskin, S., & Akveran, G. A. (2020). Effects of boric acid on proinflammation cytokines, total oxidativeantioxidative status and hematological parameters in rats applied benzo (a) pyrene. Feb Fresenıus Envıronmental Bulletin, 3599.
- Comba, B., Cinar, A., Comba, A., & GENCER, Y. G. (2016). Effects of ACTH application on kidney function tests, the electrolytes and hematological parameters in rats. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 63(3), 229-233.
- Comba, B., Oto, G., Mis, L., Özdemir, H., & Comba, A. (2016). Effects of borax on inflammation, haematological parameters and total oxidant-antioxidant status in rats applied 3– methylcholanthrene. Kafkas Univ Vet Fak Derg, 22(4), 539- 544.
- Dybing, E., Sanner, T. (2003). Risk assessment of acrylamide in foods. Toxicol. Sci., 75: 7–15.
- Eker İriş, H., & Yaşar, S. (2021). Investigation of the effect of Allium Schoenoprasum L. (sirmo) plant against acrylamide toxicity in rats. Fresenius Environmental Bulletin, vol.30, no.7, 8889-8904.
- Fırat, M., Aziret, A. (2016). Edible Allium L. species that are sold as fresh vegetables in public bazaars of Hakkâri province and its surroundings in Turkey. Acta Biologica Turcica., 29: 14-19.
- Gargas, L., Kirman, R., Sweeney, L.M., Tardiff, R.G. (2009). Acrylamide: consideration of species differences and nonlinear processes inestimating risk and safety for human ingestion. Food chem Toxicol, 47 (4): 760-768
- Haro, G., Sinaga, S. M., Iksen, I., Nerdy, N., & Theerachetmongkol, S. (2017). Protective effects of Chives Leaves (Allium Schoenoprasum, L.) infusion against ethylene glycol and ammonium chloride induced nephrolithiasis in rats. Journal of Applied Pharmaceutical Science, 7(8), 222-225.
- Hashimoto, K., Aldridge, W.N. (1970). Biochemical studies on acrylamide, a neurotoxic agent. Biochem Pharmacol, 19: 2591–604
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- Mis, L., & Oğuz, B. (2022). Anaplasma phagocytophilum in Horses - Evaluation of Proinflammatory Biomarkers. Acta Scientiae Veterinariae, 50. https://doi.org/10.22456/1679-9216.119981
- Mis, L., Baydas, B., & Yaşar, S., (2021). Evaluation of the effect of pomegranate flowers on antioxidant activity and BDNF levels in experimental renal failure in rats. Fresenius Environmental Bulletin , vol.30, no.6, 5669-5675.
- Mis, L., Comba, B., Uslu, S., & Yeltekin, A. (2018). Effect of wheatgrass on DNA damage, oxidative stress index and histological findings in diabetic rats. International journal of morphology, 36(4)
- Pedreschi, F., Kaack, K., Granby, K. (2004). Reduction of acrylamide formation in potato slices during frying. LWT-Food Sci Technol, 37: 679–85
- Pruser, K. N., Flynn, N. E. (2011). Acrylamide in healthanddisease. Front Biosci (ScholEd), 3: 41-51
- Rayburn, J.R., Friedman, M. (2010). L-Cysteine, N-Acetyl-l- cysteine, and glutathione protect xenopus laevis embryos against acrylamide-induced malformations and mortality in the frog embryo teratogenesis assay. J. Agric. Food Chem;58(20):11172-1117
- Stadler, R. H., Blank, I., Varga, N., Robert, F., Hau, J., Guy, P. A., Robert, M. C. Riedeker, S,. (2002). Food chemistry: Acrylamide from maillard reaction products. Nature, 419: 449-450.
- Stajner, D., Popovic, B. M., Calic-Dragosavac, D., Malencic, D., Zdravkovic‐Korac, S. (2011). Comparative study on Allium Schoenoprasum cultivated plant and allium schoenoprasum tissue culture organs antioxidant status. Phytother. Res: 1618- 1622.
- Stajner, D.,Canadanoviç-Brunet ,J. Pavloviç, A. (2004). Allium Schoenoprasum L., as a natural antioxidant. Phytother. Res, 18: 522–524
- Timite, G., Offer, A. C. M., Miyamoto, T., Tanaka, C., Mirjolet, J. F., Duchamp, O. (2013). Dubois MAL. Structure and cytotoxicity of steroidal glycosides from Allium schoenoprasum L. Phytochemistry, 88: 61–66.
- Yousef, M. I., El-Demerdash, F. M. (2006). Acrylamide-induced oxidative stress and biochemical perturbations in rats. Toxicology, 219 (1-3): 133-141.
The Evaluation Of Hematological Parameters After Administrated Allium Schoenoprasum L. Ethanol Extract And Acrylamide İn The Rats
Abstract
In this study, it was aimed to investigate the protective effect of Allium schoenoprasum L. plant against acrylamide toxicity. As animal material, 32 Wistar-Albino female rats were divided into 4 groups. The first group formed the control group. In the second group, 25 mg/kg Acrylamide was administered by gastric gavage every day for 15 days. In the third group, 200 mg/kg Allium schoenoprasum L. ethanol extract was given by gastric gavage for 15 days. In the fourth group, 25 mg/kg Acrylamide + 200 mg/kg Allium schoenoprasum L. ethanol extract was administered by gastric gavage for 15 days. As a result of the analysis, although the levels of RBC, HCT, HGB, MCV, MCH, MCHC WBC, Lymphocyte, Monocyte and Eosinophil Lymphocyte (%) decreased in rats in the acrylamide group, the amount of neutrophils and RDW increased (P<0.05). It was determined that Allium schoenoprasum L. plant may have an effect in correcting the negative effects of acrylamide exposure.
References
- Aamir, M., Shazma,N., Rukhsana,A., Mehwish,J., Habib,A., Asifa,B., Qurat-ul,A., Baderqa,A. (2016). Evaluation of Hypolipidemic Activity of Alliumschoenoprasum in Albino Rats. British Journal of Pharmaceutical Research, 14 (5): 1-10.
- Ali MA, Aly EM, Elawady AI. (2014). Effectiveness of selenium on acrylamide toxicity to retina.Int J Opthalmol, 7(4):614-620
- Altinoz, E., Turkoz, Y. (2014). The protective role of N-Acetylcysteine against acrylamide-ınduced genotoxicity and oxidati ve stress ın rats. Gene Ther Mol Biol , 16: 35-43.
- Barber, D.S., Hunt, J.R., Ehrich, M.F., Lehning, E.J., LoPachin, R.M. (2001). Toxicokinetics and hemoglobin adduct formation in rats following subacute and subchronic acrylamide dosing. Metabolism, 22(3):341-353.
- Boettcher, M. I., Schettgen, T., Kutting, B., Pischetsrieder, M., Angerer, J. (2005). Mercapturic acids of acrylamide and glycidamide as biomarkers of the internal exposure to acrylamide in the general population. Mutat Res, 580: 167–76.
- Ceylan, S., Cetin, S., Camadan, Y., Saral, O., Ozsen, O., Tutus, A. (2019). Antibacterial and antioxidant activities of traditional medicinal plants from the Erzurum region of Turkey. Irish Journal of Medical Science,
- Claus, A., Carle, R., Schieber, A. (2008). Acrylamide in cereal products. A review Journal of Cereal Science, 47: 118-133.
- Comba, A., Oto, G., Comba, B., Ozdemir, H., Keskin, S., & Akveran, G. A. (2020). Effects of boric acid on proinflammation cytokines, total oxidativeantioxidative status and hematological parameters in rats applied benzo (a) pyrene. Feb Fresenıus Envıronmental Bulletin, 3599.
- Comba, B., Cinar, A., Comba, A., & GENCER, Y. G. (2016). Effects of ACTH application on kidney function tests, the electrolytes and hematological parameters in rats. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 63(3), 229-233.
- Comba, B., Oto, G., Mis, L., Özdemir, H., & Comba, A. (2016). Effects of borax on inflammation, haematological parameters and total oxidant-antioxidant status in rats applied 3– methylcholanthrene. Kafkas Univ Vet Fak Derg, 22(4), 539- 544.
- Dybing, E., Sanner, T. (2003). Risk assessment of acrylamide in foods. Toxicol. Sci., 75: 7–15.
- Eker İriş, H., & Yaşar, S. (2021). Investigation of the effect of Allium Schoenoprasum L. (sirmo) plant against acrylamide toxicity in rats. Fresenius Environmental Bulletin, vol.30, no.7, 8889-8904.
- Fırat, M., Aziret, A. (2016). Edible Allium L. species that are sold as fresh vegetables in public bazaars of Hakkâri province and its surroundings in Turkey. Acta Biologica Turcica., 29: 14-19.
- Gargas, L., Kirman, R., Sweeney, L.M., Tardiff, R.G. (2009). Acrylamide: consideration of species differences and nonlinear processes inestimating risk and safety for human ingestion. Food chem Toxicol, 47 (4): 760-768
- Haro, G., Sinaga, S. M., Iksen, I., Nerdy, N., & Theerachetmongkol, S. (2017). Protective effects of Chives Leaves (Allium Schoenoprasum, L.) infusion against ethylene glycol and ammonium chloride induced nephrolithiasis in rats. Journal of Applied Pharmaceutical Science, 7(8), 222-225.
- Hashimoto, K., Aldridge, W.N. (1970). Biochemical studies on acrylamide, a neurotoxic agent. Biochem Pharmacol, 19: 2591–604
- Ko, M. H., Chen, W. P., Hsieh, S.T. (2002). Neuropathology of skin denervation in acrylamide-inducedneuropathy. Neurobiol Dis, 11 (1): 155-65.
- Konings, E.J.M, Baarsb, A.J., van Klaveren, J.D., Spanjer, M.C., Rensen, P.M., Hiemstra, M., van Kooij, J.A., Peters, P.W. (2003). Acrylamide exposure from foods of the Dutch population and an assessment of the consequent risks.;41(11):1569-1579
- Mis, L., & Oğuz, B. (2022). Anaplasma phagocytophilum in Horses - Evaluation of Proinflammatory Biomarkers. Acta Scientiae Veterinariae, 50. https://doi.org/10.22456/1679-9216.119981
- Mis, L., Baydas, B., & Yaşar, S., (2021). Evaluation of the effect of pomegranate flowers on antioxidant activity and BDNF levels in experimental renal failure in rats. Fresenius Environmental Bulletin , vol.30, no.6, 5669-5675.
- Mis, L., Comba, B., Uslu, S., & Yeltekin, A. (2018). Effect of wheatgrass on DNA damage, oxidative stress index and histological findings in diabetic rats. International journal of morphology, 36(4)
- Pedreschi, F., Kaack, K., Granby, K. (2004). Reduction of acrylamide formation in potato slices during frying. LWT-Food Sci Technol, 37: 679–85
- Pruser, K. N., Flynn, N. E. (2011). Acrylamide in healthanddisease. Front Biosci (ScholEd), 3: 41-51
- Rayburn, J.R., Friedman, M. (2010). L-Cysteine, N-Acetyl-l- cysteine, and glutathione protect xenopus laevis embryos against acrylamide-induced malformations and mortality in the frog embryo teratogenesis assay. J. Agric. Food Chem;58(20):11172-1117
- Stadler, R. H., Blank, I., Varga, N., Robert, F., Hau, J., Guy, P. A., Robert, M. C. Riedeker, S,. (2002). Food chemistry: Acrylamide from maillard reaction products. Nature, 419: 449-450.
- Stajner, D., Popovic, B. M., Calic-Dragosavac, D., Malencic, D., Zdravkovic‐Korac, S. (2011). Comparative study on Allium Schoenoprasum cultivated plant and allium schoenoprasum tissue culture organs antioxidant status. Phytother. Res: 1618- 1622.
- Stajner, D.,Canadanoviç-Brunet ,J. Pavloviç, A. (2004). Allium Schoenoprasum L., as a natural antioxidant. Phytother. Res, 18: 522–524
- Timite, G., Offer, A. C. M., Miyamoto, T., Tanaka, C., Mirjolet, J. F., Duchamp, O. (2013). Dubois MAL. Structure and cytotoxicity of steroidal glycosides from Allium schoenoprasum L. Phytochemistry, 88: 61–66.
- Yousef, M. I., El-Demerdash, F. M. (2006). Acrylamide-induced oxidative stress and biochemical perturbations in rats. Toxicology, 219 (1-3): 133-141.
There are 29 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Research Article |
| Authors | |
| Publication Date | August 31, 2023 |
| Published in Issue | Year 2023 |
