Research Article
Year 2016,
Volume: 1 Issue: 2, 47 - 52, 31.12.2016
Abstract
The Kangal dog significant richness of our country within region of Sivas is under risk at venereal diseases and unconscious mating. Animal genetic resources in-situ and ex-situ methods can be protected using. Ex-situ methods; animals outside the environment in which they live, to keep these animals or gametes, embryos, is to protect the cells or DNA. The protection of specific species such as stud Kangal dogs, possible by cryopreservation. The sperms from Kangal dogs were diluted and frozen with two different diluents in the study. Higher values were determined by the diluent supplemented with BSA in terms of the post-thawing sperm motility (%45 and %55). As a result, it is evaluated that the addition of BSA to Tris-egg yolk-based extenders in freezing of Kangal dog semen can positively affect the fertility rates after artificial insemination.
References
- 1. Blesbois E, Caffin JP. (1992): Serum like albumin of fowl seminal plasma and effects of albumin on fowl spermatozoa stored at 4 0C. British Poultry Science, 33, 663-670. 2. Concannon PW, Battista M. (1989): Canine semen freezing and artificial insemination. Current Veterinary Therapy. 1247-1259. 3. Daşkın A, Tekin N, Akçay E. (2003): Köpeklerde transservikal – intrauterin ve intravaginal tohumlama yön-temlerinin dölverimine etkisi. Turkish Journal of Veterinary And Animal Sciences, 27, 235-239. 4. De Leeuw FE, De Leeuw AM, Den Das JH, Colenbrander B, Verkleij AJ. (1993): Effect of various cryoprotective agents and membransstabilizing compounds on bull sperm membrane integrity after cooling and freezing. Cryobiology, 30, 32-44 5. England GC. (1992): Cryopreservation of dog semen: a review. Journal of reproduction and fertility. Supp. 47: 243-255. 6. Funahashi H, Cantley TC, Stumpf TT, Terlouw Sl, Day BN. (1994): Use of low salt culture medium for in vitro maturation of porcine oocytes is associated with elevated oocytes glutathione levels and enhanced male pronuclear formation after in vitro fertization. Biology of Reproduction, 51, 633-639. 7. Hermansson U, Forsberg CL. (2006): Freezing of stored, chilled dog spermatozoa. Theriogenology. 65(3): 584-593. 8. Hiemstra SJ. (2003): Guidelines for the constiution of national cryopreservation programmes for farm animals (FAO). 9. Hunter RHF. (1980): Physiology and technology of reproduction in female domestic animals. Academic Press, Edinburgh, UK. 10.Linde-Forsberg C, Forsberg M. (1989): Fertility in dogs in relation to semen quality and the time and site of insemination with fresh and frozen semen. Journal of Reproduction and Fertility. 299-310. 11.Mamak N. (2002): Sivas yöresindeki kangal köpeği üretim çiftliklerinde bulunan köpeklerde bazı enfeksiyöz ve paraziter hastalıkların (leptospirozis, listeriozis, dirofilariazis, barsak parazitleri) araştırılması ve sağaltımı. Doktora Tezi, Ankara Üniversitesi, Sağlık Bilimleri Enstitüsü. 12.Moce E, Vicente JS, Lavara R. (2003): Effect of freezing-thawing protocols on the performance of semen from three rabbit lines after artificial insemination. Theriogenology, 60, 115-123. 13.Morton DB, Bruce SG. (1989): Semen evaluation, cryopreservation and factors relevant to the use of frozen semen in dogs. Journal of Reproduction Fertility, 39, 311-316. 14.Müller B, Kircher C. (1978): Influence of seminal plasma proteins on motility of rabbit spermatozoa. Journal of Reproduction and Fertility, 54, 167-172 15.Risopatron J, Catalan S, Miska W, Schell WB, Sanchez, R. (2002): Effect of albumine and polyvinly alcohol on the vitality, motility and acrosomal integrity of canine spermatozoa ıncubated in vitro. Reproduction Domestic Animals, 37, 347-351. 16.Silva LDM, Verstegen JP. (1995): Comparisons between three different extenders for canine intrauterine insemination with frozenthawed spermatozoa. Theriogenology, 44(4), 571- 579. 17.Smith FO, Graham, EF. (1984): Cryopreservation of canine semen: technique and performance. In 10. international congress on animal reproduction and artificial insemination, University of Illinois USA. 18.Tekin N, İzgür H, Özyurt M. (1987): Köpeklerde penis masajı yöntemiyle sperma alma ve başlıca spermatolojik özellikler üzerinde araştırmalar. Selçuk Üniversitesi Veteriner Fakültesi Dergisi, 1: 83-95. 19.Tosun H. Uysal O. (2007): Köpek spermasının dondurulmasında farklı sulandırıcıların ve bireylerin etkisi. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 54:23-28. 20.Trimeche A, Anton M, Renard P, Gabdemer, G, Tainturier D. (1997): Quail egg yolk: A novel cryoprotectant for the freeze preservation of poitou jackass sperm. Cryobiology, 34, 385-393. 21.Uysal O, Korkmaz T, Tosun H. (2005): Effect of bovine serum albumine on freezing of canine semen. Indian Veterinary Journal, 82, 97-98. 22.Uysal O, Varish O, Tosun H, Yavas I, Gurcan I. (2007): Cryopreservation of canine semen at different freezing and thawing programmes. Indian veterinary journal, 84(1): 57-59. 23.Watson PF. Artificial insemination and the preservation of semen. Marshall’s Physiology of Reproduction, 2, 747-869, 1990. 24.Yurdaydın N, Kotzab E. (1987): Köpek spermasının dondurulması üzerinde araştırmalar. Ankara Üniveersitesi Veteriner Fakültesi Dergisi 34 (3): 534-540.
Year 2016,
Volume: 1 Issue: 2, 47 - 52, 31.12.2016
Abstract
Ülkemizin zenginliklerinden biri olan ve üstün özellikler barındıran Türk Çoban Köpeği Kangal (Karabaş), dünyanın en eski ve en yaygın doğal çoban köpeği ırkıdır. Fakat gerek evcilleştirmenin etkisi gerekse bilinçsiz, kontrolsüz çiftleştirmeler sebebiyle kangal köpekleri sahip olduğu üstün özelikler açısından risk altındadır. Ayrıca venereal (çiftleşme ile bulaşan) hastalıklar, damızlık köpekler için sürekli tehdit oluşturmaktadır. Bu gibi özel türler gamet, embriyo, hücre ya da DNA'ları dondurularak koruma altına alınabilmek ve kontrollü olarak yavru almak mümkündür. Köpek spermalarının dondurularak saklanmasında çeşitli yöntemler ve protokoller üzerinde çalışmalar devam etmektedir. Çalışmada Kangal ırkı köpeklerden alınan spermalar iki farklı sulandırıcı ile sulandırılmış ve dondurulmuştur. Çözüm sonu motilite oranları açısından BSA ile desteklenen sulandırıcı ile daha yüksek değerler tespit edilmiştir (%45 ve %55). Sonuç olarak Kangal Köpeği spermasının dondurulmasında Tris-yumurta sarısı bazlı sulandırıcılara BSA ilavesi yapılmasının suni tohumlama uygulamaları sonrası fertilite oranlarına olumlu etki edebileceği değerlendirilmektedir.
Keywords
References
- 1. Blesbois E, Caffin JP. (1992): Serum like albumin of fowl seminal plasma and effects of albumin on fowl spermatozoa stored at 4 0C. British Poultry Science, 33, 663-670. 2. Concannon PW, Battista M. (1989): Canine semen freezing and artificial insemination. Current Veterinary Therapy. 1247-1259. 3. Daşkın A, Tekin N, Akçay E. (2003): Köpeklerde transservikal – intrauterin ve intravaginal tohumlama yön-temlerinin dölverimine etkisi. Turkish Journal of Veterinary And Animal Sciences, 27, 235-239. 4. De Leeuw FE, De Leeuw AM, Den Das JH, Colenbrander B, Verkleij AJ. (1993): Effect of various cryoprotective agents and membransstabilizing compounds on bull sperm membrane integrity after cooling and freezing. Cryobiology, 30, 32-44 5. England GC. (1992): Cryopreservation of dog semen: a review. Journal of reproduction and fertility. Supp. 47: 243-255. 6. Funahashi H, Cantley TC, Stumpf TT, Terlouw Sl, Day BN. (1994): Use of low salt culture medium for in vitro maturation of porcine oocytes is associated with elevated oocytes glutathione levels and enhanced male pronuclear formation after in vitro fertization. Biology of Reproduction, 51, 633-639. 7. Hermansson U, Forsberg CL. (2006): Freezing of stored, chilled dog spermatozoa. Theriogenology. 65(3): 584-593. 8. Hiemstra SJ. (2003): Guidelines for the constiution of national cryopreservation programmes for farm animals (FAO). 9. Hunter RHF. (1980): Physiology and technology of reproduction in female domestic animals. Academic Press, Edinburgh, UK. 10.Linde-Forsberg C, Forsberg M. (1989): Fertility in dogs in relation to semen quality and the time and site of insemination with fresh and frozen semen. Journal of Reproduction and Fertility. 299-310. 11.Mamak N. (2002): Sivas yöresindeki kangal köpeği üretim çiftliklerinde bulunan köpeklerde bazı enfeksiyöz ve paraziter hastalıkların (leptospirozis, listeriozis, dirofilariazis, barsak parazitleri) araştırılması ve sağaltımı. Doktora Tezi, Ankara Üniversitesi, Sağlık Bilimleri Enstitüsü. 12.Moce E, Vicente JS, Lavara R. (2003): Effect of freezing-thawing protocols on the performance of semen from three rabbit lines after artificial insemination. Theriogenology, 60, 115-123. 13.Morton DB, Bruce SG. (1989): Semen evaluation, cryopreservation and factors relevant to the use of frozen semen in dogs. Journal of Reproduction Fertility, 39, 311-316. 14.Müller B, Kircher C. (1978): Influence of seminal plasma proteins on motility of rabbit spermatozoa. Journal of Reproduction and Fertility, 54, 167-172 15.Risopatron J, Catalan S, Miska W, Schell WB, Sanchez, R. (2002): Effect of albumine and polyvinly alcohol on the vitality, motility and acrosomal integrity of canine spermatozoa ıncubated in vitro. Reproduction Domestic Animals, 37, 347-351. 16.Silva LDM, Verstegen JP. (1995): Comparisons between three different extenders for canine intrauterine insemination with frozenthawed spermatozoa. Theriogenology, 44(4), 571- 579. 17.Smith FO, Graham, EF. (1984): Cryopreservation of canine semen: technique and performance. In 10. international congress on animal reproduction and artificial insemination, University of Illinois USA. 18.Tekin N, İzgür H, Özyurt M. (1987): Köpeklerde penis masajı yöntemiyle sperma alma ve başlıca spermatolojik özellikler üzerinde araştırmalar. Selçuk Üniversitesi Veteriner Fakültesi Dergisi, 1: 83-95. 19.Tosun H. Uysal O. (2007): Köpek spermasının dondurulmasında farklı sulandırıcıların ve bireylerin etkisi. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 54:23-28. 20.Trimeche A, Anton M, Renard P, Gabdemer, G, Tainturier D. (1997): Quail egg yolk: A novel cryoprotectant for the freeze preservation of poitou jackass sperm. Cryobiology, 34, 385-393. 21.Uysal O, Korkmaz T, Tosun H. (2005): Effect of bovine serum albumine on freezing of canine semen. Indian Veterinary Journal, 82, 97-98. 22.Uysal O, Varish O, Tosun H, Yavas I, Gurcan I. (2007): Cryopreservation of canine semen at different freezing and thawing programmes. Indian veterinary journal, 84(1): 57-59. 23.Watson PF. Artificial insemination and the preservation of semen. Marshall’s Physiology of Reproduction, 2, 747-869, 1990. 24.Yurdaydın N, Kotzab E. (1987): Köpek spermasının dondurulması üzerinde araştırmalar. Ankara Üniveersitesi Veteriner Fakültesi Dergisi 34 (3): 534-540.
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Details
| Primary Language | Turkish |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Research Article |
| Authors | |
| Publication Date | December 31, 2016 |
| Published in Issue | Year 2016Volume: 1 Issue: 2 |
