Research Article
Resveratrolün Metotreksat Uygulanan Ratlarda Total Oksidan-Antioksidan Durumuna ve DNA Hasarına Etkisi
Abstract
Bu çalışmada resveratrolün (RES) metotreksat (MTX) uygulanan ratlarda lökosit yüzdelerine, total oksidan (TOS)-antioksidan durumuna (TAS) ve DNA hasarına (8-OHdG) etkisi araştırıldı. Çalışmada kullanılan ratlar rastgele her biri 10 rattan oluşan 4 gruba ayrıldı. Gruplar, kontrol grubu, MTX grubu (20 mg/kg i.p., haftada 1 kez, iki hafta), RES grubu (40 mg/kg/gün oral, 14 gün) MTX+RES grubu (20 mg/kg i.p, haftada 1 kez, iki hafta ve 40 mg/kg/gün oral, 14 gün) olarak oluşturuldu. Deneme sonunda ratların kalplerinden kan örnekleri alındı, lökosit (nötrofil, eozinofil, bazofil, lenfosit, monosit) yüzdelerine klasik yöntemlerle, TOS, TAS, değerlerine kolorimetrik kitlerle, 8-OHdG seviyesine ELISA kiti ile bakıldı. MTX ve MTX+RES gruplarındaki nötrofil (p≤0.05) ve monosit (p≤0.01) yüzdeleri kontrol ve RES grubuna göre istatistiksel önemde daha düşük olduğu belirlendi. Ayrıca, MTX grubundaki, TOS ve OSI seviyelerinde anlamlı bir artış (p<0.001), TAS seviyesinde anlamlı bir azalma (p<0.05) bulundu. MTX+RES grubundaki ratlarda ise bu parametrelerin istatistiksel önemde iyileştiği belirlendi. Eozinofil, bazofil, lenfosit yüzdelerindeki değişimler istatistiksel olarak önemsizdi. Sonuç olarak, MTX in neden olduğu nötropeni ve monositopeninin iyileştirilmesinde RES’ in etkili olamayacağını fakat oksidatif stresi azaltmada etkili olabileceğini söyleyebiliriz.
Keywords
Supporting Institution
Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Proje başkanlığı
Project Number
2015-SBE-YL235
Thanks
Yüzüncü Yıl Üniversitesi Bilimsel Araştırma Proje başkanlığına desteklerinden dolayı teşekkür ederiz.
References
- Abdel-Wahaba BA, Abdel-Wahab MM (2016). Protective effect of resveratrol against chronic intermittent hypoxia-induced spatial memory deficits, hippocampal oxidative DNA damage and increased p47Phox NADPH oxidase expression in young rats. Behav Brain Res, 305, 65–75.
- Aggrawal B, Shishodia S (2005). Resveratrol: A polyphenol for all season. Resveratrol in Health and Disease. University of Texas M. D. Anderson Cancer Center, CRC Press, Taylor and Francis group, 1, 70, 1-16.
- Akkuş Ö, Şanlı K (2006). Kanserli çocuklarda hemşirelik bakımı ve desteğinin önemi. Uyar M, Uslu R, Yıldırım Y.K, editör. Kanser ve palyatif bakım. İzmir: Meta Basım Matbaacılık, 309-19.
- Alturfan AA, Tozan-Beceren A, Sehirli AO, Demiralp E, Sener G, Omurtag GZ (2011). Resveratrol ameliorates oxidative DNA damage and protects against acrylamide-induced oxidative stress in rats. Mol Biol Rep, 39, 4, 4589-4596.
- Aribal-Kocatürk P, Kavas GÖ, Büyükkağnıcı Dİ (2007). Pretreatment effect of resveratrol on streptozotocin-induced diabetes in rats. Biol Trace Elem Res, 118, 3, 244–249.
- Armağan İ (2015). Metotreksat’ın karaciğer ve böbrekte neden olduğu hasarda oksidatif stresin rolü. SDÜ Tıp Fak Derg, 22, 4, 151-155.
- Atmaca N, Atmaca HT, Kanici A, Anteplioğlu (2014). Protective effect of resveratrol on sodium fluoride-induced oxidative stress, hepatotoxicity and neurotoxicity in rats Food and Chemical Toxicology, 70: 191–197
- Babiak RM, Campello AP, Carnieri EG, Oliveira MB (1998). Methotrexate: Pentose Cycle And Oxidative Stres. Cell. Biochem. Funct. 6: 283-293.
- Blijlevens N, Sonis S (2007). Palifermin (Recombinant Keratinocyte Growth Factor-1): A Pleiotropic Growth Factor With Multiple Biological Activities In Preventing Chemotherapy- And Radiotherapy-Induced Mucositis. Ann. Oncol. 18: 817-826.
- Block KI, Koch AC, Mead MN, Tothy PK, Newman RA, Gyllenhaal C (2007). Impact of antioxidant suplementation on chemotherapeutic efficacy: A systematic review of the evidence from randomized controlled trials. Cancer Treat Rev, 33, 5, 407-418.
- Bozkurt M, Em S, Oktayoglu P, Turkcu G, Yuksel H, Sarıyıldız MA, Caglayan M, Batmaz İ, Nas K, Bozkurt Y, Kuyumcu M (2014). Carvacrol prevents methotrexateinduced renal oxidative injury and renal damage in rats Clin In_est Med, 37 (1): E19-E25.
- Burkitt MJ, Duncan J (2000). Effects of trans-resveratrol on copper-dependent hydroxylradical formation and DNA damage: evidence for hydroxyl-radical scavenging and a novel, glu-tathione-sparing mechanism of action. Arch Biochem Biophys, 15, 381, 2, 253-263
- Can G (2003). Kanser kemoterapi rehberi ve uygulamaya yönelik öneriler. Editörler; Durna Z, Aydıner A, Istanbul, Nobel tıp Kitapevleri.
- Caron, J. E., Krull, K. R., Hockenberry, M., Jain, N., Kaemingk, K., Moore, I. M. (2009). Oxidative stress and executive function in children receiving chemotherapy for acute lymphoblastic leukemia. Pediatric Blood and Cancer, 53(4), 551-556.
- Cetinkaya A, Kurutas EB, Bulbuloglu E, Kantarceken B (2007). The effects of Nacetylcysteine on methotrexate-induced oxidative renal damage in rats. Nephrol Dial Transplant. 22, 1, 284-285.
- Comba B, Cinar A, Comba A, Gencer YG, 2016a. Effects of ACTH application on kidney function tests, the electrolytes and hematological parameters in rats. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 63 (3), 229-233.
- Comba B, Mis L, Uslu S, Comba A (2017). The effect of barley grass on antioxidant capacity and DNA damage in rat with renal failure - Fresenius Environmental Bulletin, 26 10, 6135-6140.
- Comba A, Oto G, Comba B, Ozdemir H, Keskin S, Akveran Aslan G, 2020. Effects of boric acid on proinflammation cytokines, total oxidative antioxidative status and hematological parameters in rats applied benzo(a)pyrene, Fresenıus Envıronmental Bulletin, 29(5)3599-3605.
- Comba B, Oto G, Mis L, Özdemir H, Comba A 2016b. Effects of borax on inflammation, haematological parameters and total oxidant-antioxidant status in rats applied 3–methylcholanthrene - Kafkas Univ Vet Fak Derg, , 22, 4 539-544
- Çelebi S (2003). Çocuklarda Febril Nötropeni, UÜ Tıp Fak Derg; 29, 2, 35-41.
- Çetin A, Kaynar L, Koçyiğit İ, Kabukçu Hacıoğlu S, Saraymen R, Ozturk A, Ismail Sari, Sagdic O (2008). Role of grape seed extract on methotrexate ınduced oxidative stress in rat liver The American Journal of Chinese Medicine, Vol. 36, No. 5, 861–872.
- Dalaklioğlu S, Genç G.E, Aksoy N.H, Akçit F, Gümüşlü S (2013). Resveratrol ameliorates methotrexate-induced hepatotoxicity in rats via inhibition of lipid peroxidation. Human and Experimental Toxicology, The Author(s), 32(6), 662–671.
- Dilek K (2002). Günümüzde en sık kullanılan antisupresif ajanların özellikleri. Ankem Derg, 16, 3, 199-202.
- Dong Z (2003). Molecular mechanism of the chemopreventive effect of resveratrol. Mutat Res, 523-524, 145-150.
- Duman TT, Üstün C, Şavlı H (2014). Hatalı Metotreksat Kullanımı Sonucu Gelişen Nötropenik Ateş. Ankem Antibiyotik ve Kemoterapi Kongresi, Bodrum. Ankem Derg, 28 (Ek 1)
- Emılia-Juan M, Pilar-Vinardell M, Joana M (2002). The daily oral administration of high doses of trans-resveratrol to rats for 28 days is not harmful. J. Nutr, 132: 257–260.
- Erel O (2004). A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem, 37, 112-119.
- Erel O (2005). A new automated colorimetric method for measuring total oxidant status. J Clin Biochem, 47, 119-129.
- Fontacave M, Lepoivre M, Elleilgant E, Gerez C, Guittet O (1998). Resveratrol, a remarkable inhibitor of ribonucleotide reductase, FEBS Lett, 421, 277-279.
- Hadi NR, Al-Amran FG, Swadi A (2012). Metformin ameliorates methotrexate-induced hepatotoxicity.J Pharmacol Pharma-cother, 3: 248-53.
- Hempel L, Misselwitz J, Fleck C, Kentouche K, Leder C, Appenroth D et al. (2003) In_uence of high-dose methotrexate therapy (HD-MTX) on glomerular and tubular kidney function. Medical and pediatric oncology, 40(6): 348-54.
- Hung LM, Chen JK, Huang SS, Lee RS, Su MJ (2000). Cardioprotective effect of resveratrol, a natural antioxidant derived from grapes. Cardiovasc Res, 18, 47(3), 549-55.
- Ignatowicz, E., Bear-Dubowska, W., 2001. Resveratrol, a natural chemopreventive agent against degenerative diseases. Pol. J. Pharmacol, 53: 557–569.
- Jahovic N, Cevik H, Sehirli AO, Yegen BC, Sener G (2003). Melatonin prevents methotrexate-induced hepatorenal oxidative injury in rats. Journal of pineal research, 34(4): 282-7.
- Kasdallah-Grissa A, Mornagui B, Aouani E, Hammami M, El May M, Gharbi N, Kamoun A, El-Fazaa S (2007). Resveratrol, a red wine polyphenol, attenuates ethanol-induced oxidative stress in rat liver. Life Sci, 80: 1033–1039.
- Kaya E, Yılmaz S. (2020). Deneysel Olarak Metotreksat Uygulanmış Ratlarda Oksidatif Stres ve Antioksidan Enzim Aktiviteleri Üzerine Enginar Uygulamasının Etkisi]. F.Ü.Sağ.Bil.Vet.Derg. 34 (1): 43 - 47
- Kaynar L, Çetin A, Hacıoğlu KS, Eser B, Koçyiğit S, Canöz Ö, Taşdemir A, Karadağ C, Kurnaz F, Saraymen R, Silici S (2012). Effıcacy of royal jelly on Methotrekxate-Induced systemic Oxıdatıve Stress and Damage to small ıntestıne rats, Afr J Tradit Complement Altern Med. 9(3): 412-417.
- Kimura Y, Okuda H, Arichi S (1985). Effects of stilbenes on arachidonate metabolism in leukocytes. Biochim Biophys Acta, 834(2), 275-8.
- Kirimlioğlu V, Ara C, Yilmaz M, Ozgor D, Isik B, Sogutlu G (2006). Resveratrol, a red wine constituent polyphenol, protects gastric tissue against the oxidative stress in cholestatic rats. Dig Dis Sci, 51, 298-302.
- Kocaman N, Çolakoğlu N (2013). Tekrarlayan Dozlarda Metotreksat Uygulamasının Sıçan Karaciğer Dokusu Üzerine Etkileri, Firat Med J 18(3), 141-145.
- Kuhnle G, Spencer JP, Chowrimootoo G, Schroeter H, Debnam ES, Srai SK, et al (2000). Resveratrol is absorbed in the small in testine as resveratrol glucuronide. Biochem Biophys Res Commun, 272, 1, 212-217.
- Kolouchova-Hanzlikova I, Melzoch K, Filip V, Smidrkal J (2004). Rapid method for resveratrol determination by HPLC with electrochemical and UV detections in wines. Food Chem. 87, 151–158.
- Mis L, Comba B, Basbugan Y, Yasar S, Comba A (2019) Assesment of the wheatgrass effects on antıoxıdant capacıty and DNA damage ın rats wıth experımental renal faılure, 28 (4)2876-2879.
- Mis L, Comba B, Uslu S, Yeltekin A (2018). Effect of wheatgrass on DNA damage, oxidative stress index and histological findings in diabetic rats. Int. J. Morphol., 36(4):1235-1240
- Miyazono Y, Gao F, Horie T (2004). Oxidative stress contributes to methotrexate-induced small intestinal toxicity in rats. Scandinavian journal of gastroenterology, 39(11): 1119-27.
- Mokni M, Elkahoui S, Limam F, Amri M, Aouani E (2007). Effect of resveratrol on antioxidant enzyme activities in the brain of healthy rat. Neurochem. Res, 32: 981–987.
- Murcia MA, Martinez-Tome M (2001). Antioxidant activity of resveratrol compared with common food additives. J Food Prot, 64, 3, 379-384.
- Ohasone Y, Okano Y, Kameda H, Hama N, Matsumura M, Nojima T et al. (1997) Toxicity of low-dose methotrexate in rheumatoid arthritis –clinical characteristics in patients with MTX-induced pancytopenia and interstitial pneumonitis. Ryumachi, 37(1): 16-23.
- Sancar A, Lindsey-Boltz LA, Kang TH, Reardon JT, Lee JH, Ozturk N (2010). Circadian clock control of the cellular response to DNA damage. FEBS Letters 584 2618–2625.
- Signorelli P, Ghidoni R (2005). Resveratrol as an anticancer nutrient: moleculer basis, open questions and promises. J Nutr Biochem, 16, 449-466
- Sonis ST (2007). Pathobiology Of Oral Mucositis: Novel Insights And Opportunities. J. Support. Oncol. 5: 3-11.
- Şenol E (2004). Son bir yılda febril nötropenide ne oldu? etkenler epidemiyoloji (literatür değerlendirilmesi). Üçüncü Febril Nötropeni Mezuniyet Sonrası Eğitim Kursu. 27–29 Şubat. Bilkent Oteli, Ankara.
- Taş A, Karasu A, Comba B, Aksu D, Düz E. 2011. Effects of sildenafil citrate on the hematological parameters in the early phase of wound healing in diabetic rats.Asian Journal of Animal and Veterinary Advances, 6, 3. 290-296.
- Tatlidede E, Sehirli O, Velioğlu-Oğünc A, Cetinel S. 2009. Resveratrol treatment protects against doxorubicin-induced cardiotoxicity by alleviating oxidative damage. Free Radic Res; 43: 195- 205.
- Tian H, Cronstein BN (2007). Understanding the mechanisms of action of methotrexate: implications for the treatment of rheumatoid arthritis. Bulletin of the NYU hospital for joint diseases, 65(3):168-73.
- Tunalı-Akbay T, Şehirli Ö, Ercan F, Şener G (2010). Resveratrol Protects Against Methotreksate-İnduced Hepatic İnjury in rats. J Pharm Pharmaceut Sci ,13(2), 303-310.
- Uguralp S, Usta U, Mizrak B (2005). Resveratrol may reduce apoptosis of rat testicular germ cells after experimental testicular torsion. Eur J Pediatr Surg, 15, 333-336.
- Uraz S, Tahan V, Aygun C (2008). Role of ursodeoxycholic acid in prevention of methotrexate-induced liver toxicity. Dig Dis Sci, 53: 1071-77.
- Uzar E, Koyuncuoglu HR, Uz E, Yilmaz HR, Kutluhan S, Kilbas S et al. (2006) E activities of antioxidant enzymes and the level of malondialdehyde in cerebellum of rats subjected to methotrexate: protective efect of cafeic acid phenethyl ester. Molecular and cellular biochemistry, 291(1-2): 63-8.
- Widemann BC, Adamson PC (2006). Understanding and managing methotrexate nephrotoxicity. Oncologist, 11(6):694-703.
- Wildburger R, Mrakovcic L, Stroser M, Andrisic L, Borovic Sunjic S, Zarkovic K, Zarkovic N (2009). Lipid peroxidation and age-associated diseases-cause or consequence? Review Citation. Türkiye Klinikleri Tıp Bilimleri Derg, 29, 1, 189-193.
- Yan Y, Yang JY, Mou YH, Wang LH, Zhou YN, Wu CF (2012). Differences in the activities of resveratrol and ascorbic acid in protection of ethanol-induced oxidative DNA damage in human peripheral lymphocytes. Food Chem. Toxicol, 50: 168–174.
- Yarris JP, Hunter AJ. Roy Hertz (2003). M.D. (1909-2002): The cure of choriocarcinoma and its impact on the development of chemotherapy for cancer. Gynecologic oncology, 89(2): 193-8.
- Yıldırım S, Oto G, Comba B, Ekin S, Cinar DA (2017). An investigation of the protective effects of resveratrol on some biochemical parameters and histopathological findings in experimentally-induced chronic flurosis in rats. Fluoride, , 50, 3 365-373
- Yoon KH, Ng SC (2001). Early onset methotrexate-induced pancytopenia and response to GCSF: areport of two cases. J Clin Rheumatol, 7: 17-20.
- Zini R, Morin C, Bertelli A, Bertelli AA, Tillement JP (1999). Effects of resveratrol on the rat brain respiratory chain. Drugs Exp. Clin. Res, 25: 87–97.
- Zou Y, Van HB (1999). Strand opening by the uvra(2)b complex allows dynamic recognition of DNA damage. Embo J. 18(17):4889-901.
Effect of Resveratrol on Total Oxidative-Antioxidative Status and DNA Damage in Rats Induced Methotrexate
Abstract
In this study, the effects of resveratrol (RES) on leukocyte percentages, total oxidant (TOS) -antioxidan status (TAS) and DNA damage (8-OHDG) were investigated in rats induced by methotrexate (MTX). The rats used in the study were randomly divided into 4 groups each consisting of 10 rats.Control group , MTX group (20 mg / kg ip, once a week, for two weeks) ,RES group (40 mg / kg / day orally, for two weeks), MTX + RES group (20 mg / kg ip, once a week and 40 mg / kg / day orally for 2 weeks)At the end of the experiment, blood samples were taken from the hearts of the rats and the percentage of leukocyte (neutrophil, eosinophil, basophil, lymphocyte, monocyte) was examined by classical methods. The levels of TOS and TAS were measured by colorimetric kits and 8-OHDG level by ELISA kit. Neutrophil (p≤0.05) and monocyte (p≤0.01) percentages of MTX and MTX + RES groups were found to be statistically lower than control and RES groups. There was also a significant increase in TOS and OSI levels (p <0.001) and a significant decrease in TAS level (p <0.05) in the MTX group. In the MTX + RES group, the positive statistical significance of these parameters was determined. Changes in eosinophil, basophil, lymphocyte percentages were statistically insignificant. In conclusion, it can be said that the RES treatment for neutropenia and monocytopenia caused by MTX can not be effective but may be effective in reducing oxidative stress.
Keywords
Project Number
2015-SBE-YL235
References
- Abdel-Wahaba BA, Abdel-Wahab MM (2016). Protective effect of resveratrol against chronic intermittent hypoxia-induced spatial memory deficits, hippocampal oxidative DNA damage and increased p47Phox NADPH oxidase expression in young rats. Behav Brain Res, 305, 65–75.
- Aggrawal B, Shishodia S (2005). Resveratrol: A polyphenol for all season. Resveratrol in Health and Disease. University of Texas M. D. Anderson Cancer Center, CRC Press, Taylor and Francis group, 1, 70, 1-16.
- Akkuş Ö, Şanlı K (2006). Kanserli çocuklarda hemşirelik bakımı ve desteğinin önemi. Uyar M, Uslu R, Yıldırım Y.K, editör. Kanser ve palyatif bakım. İzmir: Meta Basım Matbaacılık, 309-19.
- Alturfan AA, Tozan-Beceren A, Sehirli AO, Demiralp E, Sener G, Omurtag GZ (2011). Resveratrol ameliorates oxidative DNA damage and protects against acrylamide-induced oxidative stress in rats. Mol Biol Rep, 39, 4, 4589-4596.
- Aribal-Kocatürk P, Kavas GÖ, Büyükkağnıcı Dİ (2007). Pretreatment effect of resveratrol on streptozotocin-induced diabetes in rats. Biol Trace Elem Res, 118, 3, 244–249.
- Armağan İ (2015). Metotreksat’ın karaciğer ve böbrekte neden olduğu hasarda oksidatif stresin rolü. SDÜ Tıp Fak Derg, 22, 4, 151-155.
- Atmaca N, Atmaca HT, Kanici A, Anteplioğlu (2014). Protective effect of resveratrol on sodium fluoride-induced oxidative stress, hepatotoxicity and neurotoxicity in rats Food and Chemical Toxicology, 70: 191–197
- Babiak RM, Campello AP, Carnieri EG, Oliveira MB (1998). Methotrexate: Pentose Cycle And Oxidative Stres. Cell. Biochem. Funct. 6: 283-293.
- Blijlevens N, Sonis S (2007). Palifermin (Recombinant Keratinocyte Growth Factor-1): A Pleiotropic Growth Factor With Multiple Biological Activities In Preventing Chemotherapy- And Radiotherapy-Induced Mucositis. Ann. Oncol. 18: 817-826.
- Block KI, Koch AC, Mead MN, Tothy PK, Newman RA, Gyllenhaal C (2007). Impact of antioxidant suplementation on chemotherapeutic efficacy: A systematic review of the evidence from randomized controlled trials. Cancer Treat Rev, 33, 5, 407-418.
- Bozkurt M, Em S, Oktayoglu P, Turkcu G, Yuksel H, Sarıyıldız MA, Caglayan M, Batmaz İ, Nas K, Bozkurt Y, Kuyumcu M (2014). Carvacrol prevents methotrexateinduced renal oxidative injury and renal damage in rats Clin In_est Med, 37 (1): E19-E25.
- Burkitt MJ, Duncan J (2000). Effects of trans-resveratrol on copper-dependent hydroxylradical formation and DNA damage: evidence for hydroxyl-radical scavenging and a novel, glu-tathione-sparing mechanism of action. Arch Biochem Biophys, 15, 381, 2, 253-263
- Can G (2003). Kanser kemoterapi rehberi ve uygulamaya yönelik öneriler. Editörler; Durna Z, Aydıner A, Istanbul, Nobel tıp Kitapevleri.
- Caron, J. E., Krull, K. R., Hockenberry, M., Jain, N., Kaemingk, K., Moore, I. M. (2009). Oxidative stress and executive function in children receiving chemotherapy for acute lymphoblastic leukemia. Pediatric Blood and Cancer, 53(4), 551-556.
- Cetinkaya A, Kurutas EB, Bulbuloglu E, Kantarceken B (2007). The effects of Nacetylcysteine on methotrexate-induced oxidative renal damage in rats. Nephrol Dial Transplant. 22, 1, 284-285.
- Comba B, Cinar A, Comba A, Gencer YG, 2016a. Effects of ACTH application on kidney function tests, the electrolytes and hematological parameters in rats. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 63 (3), 229-233.
- Comba B, Mis L, Uslu S, Comba A (2017). The effect of barley grass on antioxidant capacity and DNA damage in rat with renal failure - Fresenius Environmental Bulletin, 26 10, 6135-6140.
- Comba A, Oto G, Comba B, Ozdemir H, Keskin S, Akveran Aslan G, 2020. Effects of boric acid on proinflammation cytokines, total oxidative antioxidative status and hematological parameters in rats applied benzo(a)pyrene, Fresenıus Envıronmental Bulletin, 29(5)3599-3605.
- Comba B, Oto G, Mis L, Özdemir H, Comba A 2016b. Effects of borax on inflammation, haematological parameters and total oxidant-antioxidant status in rats applied 3–methylcholanthrene - Kafkas Univ Vet Fak Derg, , 22, 4 539-544
- Çelebi S (2003). Çocuklarda Febril Nötropeni, UÜ Tıp Fak Derg; 29, 2, 35-41.
- Çetin A, Kaynar L, Koçyiğit İ, Kabukçu Hacıoğlu S, Saraymen R, Ozturk A, Ismail Sari, Sagdic O (2008). Role of grape seed extract on methotrexate ınduced oxidative stress in rat liver The American Journal of Chinese Medicine, Vol. 36, No. 5, 861–872.
- Dalaklioğlu S, Genç G.E, Aksoy N.H, Akçit F, Gümüşlü S (2013). Resveratrol ameliorates methotrexate-induced hepatotoxicity in rats via inhibition of lipid peroxidation. Human and Experimental Toxicology, The Author(s), 32(6), 662–671.
- Dilek K (2002). Günümüzde en sık kullanılan antisupresif ajanların özellikleri. Ankem Derg, 16, 3, 199-202.
- Dong Z (2003). Molecular mechanism of the chemopreventive effect of resveratrol. Mutat Res, 523-524, 145-150.
- Duman TT, Üstün C, Şavlı H (2014). Hatalı Metotreksat Kullanımı Sonucu Gelişen Nötropenik Ateş. Ankem Antibiyotik ve Kemoterapi Kongresi, Bodrum. Ankem Derg, 28 (Ek 1)
- Emılia-Juan M, Pilar-Vinardell M, Joana M (2002). The daily oral administration of high doses of trans-resveratrol to rats for 28 days is not harmful. J. Nutr, 132: 257–260.
- Erel O (2004). A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem, 37, 112-119.
- Erel O (2005). A new automated colorimetric method for measuring total oxidant status. J Clin Biochem, 47, 119-129.
- Fontacave M, Lepoivre M, Elleilgant E, Gerez C, Guittet O (1998). Resveratrol, a remarkable inhibitor of ribonucleotide reductase, FEBS Lett, 421, 277-279.
- Hadi NR, Al-Amran FG, Swadi A (2012). Metformin ameliorates methotrexate-induced hepatotoxicity.J Pharmacol Pharma-cother, 3: 248-53.
- Hempel L, Misselwitz J, Fleck C, Kentouche K, Leder C, Appenroth D et al. (2003) In_uence of high-dose methotrexate therapy (HD-MTX) on glomerular and tubular kidney function. Medical and pediatric oncology, 40(6): 348-54.
- Hung LM, Chen JK, Huang SS, Lee RS, Su MJ (2000). Cardioprotective effect of resveratrol, a natural antioxidant derived from grapes. Cardiovasc Res, 18, 47(3), 549-55.
- Ignatowicz, E., Bear-Dubowska, W., 2001. Resveratrol, a natural chemopreventive agent against degenerative diseases. Pol. J. Pharmacol, 53: 557–569.
- Jahovic N, Cevik H, Sehirli AO, Yegen BC, Sener G (2003). Melatonin prevents methotrexate-induced hepatorenal oxidative injury in rats. Journal of pineal research, 34(4): 282-7.
- Kasdallah-Grissa A, Mornagui B, Aouani E, Hammami M, El May M, Gharbi N, Kamoun A, El-Fazaa S (2007). Resveratrol, a red wine polyphenol, attenuates ethanol-induced oxidative stress in rat liver. Life Sci, 80: 1033–1039.
- Kaya E, Yılmaz S. (2020). Deneysel Olarak Metotreksat Uygulanmış Ratlarda Oksidatif Stres ve Antioksidan Enzim Aktiviteleri Üzerine Enginar Uygulamasının Etkisi]. F.Ü.Sağ.Bil.Vet.Derg. 34 (1): 43 - 47
- Kaynar L, Çetin A, Hacıoğlu KS, Eser B, Koçyiğit S, Canöz Ö, Taşdemir A, Karadağ C, Kurnaz F, Saraymen R, Silici S (2012). Effıcacy of royal jelly on Methotrekxate-Induced systemic Oxıdatıve Stress and Damage to small ıntestıne rats, Afr J Tradit Complement Altern Med. 9(3): 412-417.
- Kimura Y, Okuda H, Arichi S (1985). Effects of stilbenes on arachidonate metabolism in leukocytes. Biochim Biophys Acta, 834(2), 275-8.
- Kirimlioğlu V, Ara C, Yilmaz M, Ozgor D, Isik B, Sogutlu G (2006). Resveratrol, a red wine constituent polyphenol, protects gastric tissue against the oxidative stress in cholestatic rats. Dig Dis Sci, 51, 298-302.
- Kocaman N, Çolakoğlu N (2013). Tekrarlayan Dozlarda Metotreksat Uygulamasının Sıçan Karaciğer Dokusu Üzerine Etkileri, Firat Med J 18(3), 141-145.
- Kuhnle G, Spencer JP, Chowrimootoo G, Schroeter H, Debnam ES, Srai SK, et al (2000). Resveratrol is absorbed in the small in testine as resveratrol glucuronide. Biochem Biophys Res Commun, 272, 1, 212-217.
- Kolouchova-Hanzlikova I, Melzoch K, Filip V, Smidrkal J (2004). Rapid method for resveratrol determination by HPLC with electrochemical and UV detections in wines. Food Chem. 87, 151–158.
- Mis L, Comba B, Basbugan Y, Yasar S, Comba A (2019) Assesment of the wheatgrass effects on antıoxıdant capacıty and DNA damage ın rats wıth experımental renal faılure, 28 (4)2876-2879.
- Mis L, Comba B, Uslu S, Yeltekin A (2018). Effect of wheatgrass on DNA damage, oxidative stress index and histological findings in diabetic rats. Int. J. Morphol., 36(4):1235-1240
- Miyazono Y, Gao F, Horie T (2004). Oxidative stress contributes to methotrexate-induced small intestinal toxicity in rats. Scandinavian journal of gastroenterology, 39(11): 1119-27.
- Mokni M, Elkahoui S, Limam F, Amri M, Aouani E (2007). Effect of resveratrol on antioxidant enzyme activities in the brain of healthy rat. Neurochem. Res, 32: 981–987.
- Murcia MA, Martinez-Tome M (2001). Antioxidant activity of resveratrol compared with common food additives. J Food Prot, 64, 3, 379-384.
- Ohasone Y, Okano Y, Kameda H, Hama N, Matsumura M, Nojima T et al. (1997) Toxicity of low-dose methotrexate in rheumatoid arthritis –clinical characteristics in patients with MTX-induced pancytopenia and interstitial pneumonitis. Ryumachi, 37(1): 16-23.
- Sancar A, Lindsey-Boltz LA, Kang TH, Reardon JT, Lee JH, Ozturk N (2010). Circadian clock control of the cellular response to DNA damage. FEBS Letters 584 2618–2625.
- Signorelli P, Ghidoni R (2005). Resveratrol as an anticancer nutrient: moleculer basis, open questions and promises. J Nutr Biochem, 16, 449-466
- Sonis ST (2007). Pathobiology Of Oral Mucositis: Novel Insights And Opportunities. J. Support. Oncol. 5: 3-11.
- Şenol E (2004). Son bir yılda febril nötropenide ne oldu? etkenler epidemiyoloji (literatür değerlendirilmesi). Üçüncü Febril Nötropeni Mezuniyet Sonrası Eğitim Kursu. 27–29 Şubat. Bilkent Oteli, Ankara.
- Taş A, Karasu A, Comba B, Aksu D, Düz E. 2011. Effects of sildenafil citrate on the hematological parameters in the early phase of wound healing in diabetic rats.Asian Journal of Animal and Veterinary Advances, 6, 3. 290-296.
- Tatlidede E, Sehirli O, Velioğlu-Oğünc A, Cetinel S. 2009. Resveratrol treatment protects against doxorubicin-induced cardiotoxicity by alleviating oxidative damage. Free Radic Res; 43: 195- 205.
- Tian H, Cronstein BN (2007). Understanding the mechanisms of action of methotrexate: implications for the treatment of rheumatoid arthritis. Bulletin of the NYU hospital for joint diseases, 65(3):168-73.
- Tunalı-Akbay T, Şehirli Ö, Ercan F, Şener G (2010). Resveratrol Protects Against Methotreksate-İnduced Hepatic İnjury in rats. J Pharm Pharmaceut Sci ,13(2), 303-310.
- Uguralp S, Usta U, Mizrak B (2005). Resveratrol may reduce apoptosis of rat testicular germ cells after experimental testicular torsion. Eur J Pediatr Surg, 15, 333-336.
- Uraz S, Tahan V, Aygun C (2008). Role of ursodeoxycholic acid in prevention of methotrexate-induced liver toxicity. Dig Dis Sci, 53: 1071-77.
- Uzar E, Koyuncuoglu HR, Uz E, Yilmaz HR, Kutluhan S, Kilbas S et al. (2006) E activities of antioxidant enzymes and the level of malondialdehyde in cerebellum of rats subjected to methotrexate: protective efect of cafeic acid phenethyl ester. Molecular and cellular biochemistry, 291(1-2): 63-8.
- Widemann BC, Adamson PC (2006). Understanding and managing methotrexate nephrotoxicity. Oncologist, 11(6):694-703.
- Wildburger R, Mrakovcic L, Stroser M, Andrisic L, Borovic Sunjic S, Zarkovic K, Zarkovic N (2009). Lipid peroxidation and age-associated diseases-cause or consequence? Review Citation. Türkiye Klinikleri Tıp Bilimleri Derg, 29, 1, 189-193.
- Yan Y, Yang JY, Mou YH, Wang LH, Zhou YN, Wu CF (2012). Differences in the activities of resveratrol and ascorbic acid in protection of ethanol-induced oxidative DNA damage in human peripheral lymphocytes. Food Chem. Toxicol, 50: 168–174.
- Yarris JP, Hunter AJ. Roy Hertz (2003). M.D. (1909-2002): The cure of choriocarcinoma and its impact on the development of chemotherapy for cancer. Gynecologic oncology, 89(2): 193-8.
- Yıldırım S, Oto G, Comba B, Ekin S, Cinar DA (2017). An investigation of the protective effects of resveratrol on some biochemical parameters and histopathological findings in experimentally-induced chronic flurosis in rats. Fluoride, , 50, 3 365-373
- Yoon KH, Ng SC (2001). Early onset methotrexate-induced pancytopenia and response to GCSF: areport of two cases. J Clin Rheumatol, 7: 17-20.
- Zini R, Morin C, Bertelli A, Bertelli AA, Tillement JP (1999). Effects of resveratrol on the rat brain respiratory chain. Drugs Exp. Clin. Res, 25: 87–97.
- Zou Y, Van HB (1999). Strand opening by the uvra(2)b complex allows dynamic recognition of DNA damage. Embo J. 18(17):4889-901.
There are 67 citations in total.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Veterinary Surgery |
| Journal Section | Research Article |
| Authors | |
| Project Number | 2015-SBE-YL235 |
| Early Pub Date | August 29, 2022 |
| Publication Date | August 31, 2022 |
| Published in Issue | Year 2022Volume: 7 Issue: 2 |
