Research Article
Determination of serum Leptin levels in cattle, sheep, goats and buffaloes in Burdur province in Türkiye by ELISA method
Abstract
Leptin is a hormone that provides the central and/or peripheral regulation of food intake, energy expenditure, whole body energy balance, fertility and immune functions by creating a feeling of satiety in animals and humans. This study was carried out to determine serum leptin values for some ruminant species and breeds that were healthy and had at least one birth. In the study, 15 Simental and 15 Holstein cows, 15 Italian buffalo, 15 Pırlak sheep, 15 Honamlı and 15 Turkish hair goats were used as material. Serum leptin concentrations were measured with the BT LAB Bovine Leptin ELISA kit. Serum leptin levels were determined as 2.78±0.22 ng/ml in Simmental cows, 2.87±0.11 ng/ml in Holstein cows, 3.6±0.48 ng/ml in Pırlak sheep, 3.38±0.76 ng/ml in Honamlı goats, 5.48±0.92 ng/ml in Hair goats and 2.50±0.17 ng/ml in Italian buffaloes. As a result, it was determined that serum leptin levels obtained from different ruminant species that were healthy and gave at least one birth in Burdur province were within the reference values reported for ruminants.
Keywords
References
- Abbas N, Suleman M, Zahur AB et al (2019). Molecular Analysis of Leptin Gene Polymorphism in Achai, Sahiwal Cattle and Nili-ravi Buffalo Breeds of Pakistan. International Journal of Genetics and Genomics 7(3): 75-79.
- Avci G, Kuçukkurt İ, Kontaş T et al (2013). The effect of dietary zinc supplementation on plasma leptin, insulin and thyroid hormone levels and some biochemical parameters in sheep of different breeds. Ankara Univ vet fac journal. 60:1-5.
- Avondo M, Trana AD, Valenti B et al (2019). Leptin Gene Polymorphism in Goats Fed with Diet at Different Energy Level: Effects on Feed Intake, Milk Traits, Milk Fatty Acids Composition, and Metabolic State. Animals 9(7): 424-433.
- Baile CA, Della-Fera MA, Martin RJ (2000). Regulation of metabolism and body fat mass by leptin. Annual Review of Nutrition, 20: 105-127.
- Blache D, Tellam RL, Chagas LM et al (2000). Level of nutrition affects leptin concentrations in plasma and cerebrospinal fluid in sheep. J Endocrinol, 165:625-637.
- Chelikani PK, Glimm DR, Kennelly JJ (2003). Short communication: tissue distribution of leptin and leptin receptor mRNA in the bovine. J Dairy Sci 2003; 86:2369-72.
- Chilliard Y, Bonnet M, Delavaud C et al (2001). Leptin in ruminants. Gene expression in adipose tissue and mammary gland, and regulation of plasma concentration. Domest Anim Endocrinol 21: 271-95.
- Chilliard Y, Ferlay A, Faulconnier Y et al (2000). Adipose tissue metabolism and its role in adaptations to undernutrition in ruminants. Proceedings of The Nutrition Society, 59: 127-134.
- Comba A. (2014). Determination of leptin and lipid profile levels in different sheep breeds. YYU. Doctoral thesis of the Institute of Health Sciences, Department of Biochemistry, Van.
- Comba B, Karakus K, Comba A. (2015). Use of Ghrelin and Leptin Hormones in Livestock. Igdir Univ J Inst sci & Tech 5(1):99-105.
- Das A, Sahin T, Akbulut O et al (2019). Determination of Leptin, Ghrelin and Insulin-Like Growth Factor-1 (IGF-1) Gene Polymorphisms in Cultured Meat Breed Cattle in Turkey. Harran Univ Vet Fak Journal, 8 (1):112-119.
- Delavaud C, Ferlay A, Faulconnier Y et al (2002). Plasma leptin concentration in adult cattle: Effects of breed, adiposity, feding level and meal intake. J Anim Sci, 80:1317-1328.
- Ferchichi MA, Jemmali B, Amiri S et al (2018) Effect of leptin genetic polymorphism on lameness prevalence in Tunisian Holstein cows. Arch Anim Breed, 61: 305–310.
- Guzel S, Tanriverdi M ve Gunes N (2012). Serum leptin concentration in some ruminant species and breeds. Journal of Animal and Veterinary Advances. 11(15):2753-2755.
- Ibrahim AHM, Ghoneim E, Faid-Allah E (2020). Barkı keçilerinde leptin gen polimorfizmlerinin süt üretim özellikleri üzerine etkisi. Egyptian Journal of Sheep & Goat Sciences. 15(3):13-22.
- Kaplan S (2018) Characterization of Bubaline Leptin Gene Polymorphism in Anatolian Buffaloes By Using PCR-RFLP Method. Alinteri Journal of Agriculture Sciences. 33(1): 93-97.
- Klaus S (2004). Adipose tissue as a regulatorof energy balance. Curr Drug Targets, 5: 241-50.
- Magistrelli DL, Pinotti L, Rapetti L et al (2011) Ghrelin, insülin and pancreatic activity in tehe periweanng period of goad kids. J Anim Physiol Anim Nutr, 95: 40-46.
- Mahrous KF, Aboelenin MM, Rashed MA et al (2020). Detection of polymorphism within leptin gene in Egyptian river buffalo and predict its effects on different molecular levels. J Genet Eng Biotechnol Dec; 18: 6.
- McFadin EL, Morrison CD, Buff PR et al (2002). Leptin concentrations in periparturient ewes and their subsequent offspring. J Anim Sci, 80:738-743.
- Schoeller DA, Cella LK, Sinha MK, Caro JF (1997). Entrainment of the diurnal rhythm of plasma leptin to meal timing. J Clin Invest, 100: 1882–1887.
- Sedykh TA, Kalashnikova LA, Gizatullin RS et al (2020). Effects of Leptin Gene Polymorphism on Beef Cattle Performance. Russian agricultural sciences. 46 (6): 614-618.
- Tajik J and Nazifi S (2011). A study of correlation of serum leptin with trace elements in water buffalo (Bubalus bubalis). Pakistan Veterinary Journal. 31(3):231-214.
- Thomas MG, Enns RM, Hallford DM et al (2002). Relationships of metabolic hormones and serum glucose to growth and reproductive development in performance-tested Angus, Brangus and Brahman bulls. J Anim Sci, 80:757-767.
- Topal CA (2004). Serum leptin levels in patients with breast cancer and their relationship with histological parameters. Master thesis. Ministry of Health Dr. Lütfi Kırdar Kartal Training and Research Hospital, Department of Internal Medicine, Istanbul.
- Yonekura S, Kitade K, Furukawa G et al (2002). Effects of aging and weaning on mRNA expression of leptin and CCK receptors in the calf rumen and abomasum. Domest Anim Endocrinol 22: 25–35.
- Zieba DA, Amstalden M, Morton S et al (2004). Regulatory roles of leptin at the hypothalamic-hypopyseal axis before and after sexual maturation in catlle. Biol Reprod 71:804-812.
Determination of Serum Leptin Levels in Cattle, Sheep, Goats and Buffaloes in Burdur Province in Türkiye by ELISA Method
Abstract
Leptin is a hormone that provides the central and/or peripheral regulation of food intake, energy expenditure, whole body energy balance, fertility and immune functions by creating a feeling of satiety in animals and humans. This study was carried out to determine serum leptin values for some ruminant species and breeds that were healthy and had at least one birth. In the study, 15 Simental and 15 Holstein cows, 15 Italian buffalo, 15 Pırlak sheep, 15 Honamlı and 15 Turkish hair goats were used as material. Serum leptin concentrations were measured with the BT LAB Bovine Leptin ELISA kit. Serum leptin levels were determined as 2.78±0.22 ng/ml in Simmental cows, 2.87±0.11 ng/ml in Holstein cows, 3.6±0.48 ng/ml in Pırlak sheep, 3.38±0.76 ng/ml in Honamlı goats, 5.48±0.92 ng/ml in Hair goats and 2.50±0.17 ng/ml in Italian buffaloes. As a result, it was determined that serum leptin levels obtained from different ruminant species that were healthy and gave at least one birth in Burdur province were within the reference values reported for ruminants.
Keywords
References
- Abbas N, Suleman M, Zahur AB et al (2019). Molecular Analysis of Leptin Gene Polymorphism in Achai, Sahiwal Cattle and Nili-ravi Buffalo Breeds of Pakistan. International Journal of Genetics and Genomics 7(3): 75-79.
- Avci G, Kuçukkurt İ, Kontaş T et al (2013). The effect of dietary zinc supplementation on plasma leptin, insulin and thyroid hormone levels and some biochemical parameters in sheep of different breeds. Ankara Univ vet fac journal. 60:1-5.
- Avondo M, Trana AD, Valenti B et al (2019). Leptin Gene Polymorphism in Goats Fed with Diet at Different Energy Level: Effects on Feed Intake, Milk Traits, Milk Fatty Acids Composition, and Metabolic State. Animals 9(7): 424-433.
- Baile CA, Della-Fera MA, Martin RJ (2000). Regulation of metabolism and body fat mass by leptin. Annual Review of Nutrition, 20: 105-127.
- Blache D, Tellam RL, Chagas LM et al (2000). Level of nutrition affects leptin concentrations in plasma and cerebrospinal fluid in sheep. J Endocrinol, 165:625-637.
- Chelikani PK, Glimm DR, Kennelly JJ (2003). Short communication: tissue distribution of leptin and leptin receptor mRNA in the bovine. J Dairy Sci 2003; 86:2369-72.
- Chilliard Y, Bonnet M, Delavaud C et al (2001). Leptin in ruminants. Gene expression in adipose tissue and mammary gland, and regulation of plasma concentration. Domest Anim Endocrinol 21: 271-95.
- Chilliard Y, Ferlay A, Faulconnier Y et al (2000). Adipose tissue metabolism and its role in adaptations to undernutrition in ruminants. Proceedings of The Nutrition Society, 59: 127-134.
- Comba A. (2014). Determination of leptin and lipid profile levels in different sheep breeds. YYU. Doctoral thesis of the Institute of Health Sciences, Department of Biochemistry, Van.
- Comba B, Karakus K, Comba A. (2015). Use of Ghrelin and Leptin Hormones in Livestock. Igdir Univ J Inst sci & Tech 5(1):99-105.
- Das A, Sahin T, Akbulut O et al (2019). Determination of Leptin, Ghrelin and Insulin-Like Growth Factor-1 (IGF-1) Gene Polymorphisms in Cultured Meat Breed Cattle in Turkey. Harran Univ Vet Fak Journal, 8 (1):112-119.
- Delavaud C, Ferlay A, Faulconnier Y et al (2002). Plasma leptin concentration in adult cattle: Effects of breed, adiposity, feding level and meal intake. J Anim Sci, 80:1317-1328.
- Ferchichi MA, Jemmali B, Amiri S et al (2018) Effect of leptin genetic polymorphism on lameness prevalence in Tunisian Holstein cows. Arch Anim Breed, 61: 305–310.
- Guzel S, Tanriverdi M ve Gunes N (2012). Serum leptin concentration in some ruminant species and breeds. Journal of Animal and Veterinary Advances. 11(15):2753-2755.
- Ibrahim AHM, Ghoneim E, Faid-Allah E (2020). Barkı keçilerinde leptin gen polimorfizmlerinin süt üretim özellikleri üzerine etkisi. Egyptian Journal of Sheep & Goat Sciences. 15(3):13-22.
- Kaplan S (2018) Characterization of Bubaline Leptin Gene Polymorphism in Anatolian Buffaloes By Using PCR-RFLP Method. Alinteri Journal of Agriculture Sciences. 33(1): 93-97.
- Klaus S (2004). Adipose tissue as a regulatorof energy balance. Curr Drug Targets, 5: 241-50.
- Magistrelli DL, Pinotti L, Rapetti L et al (2011) Ghrelin, insülin and pancreatic activity in tehe periweanng period of goad kids. J Anim Physiol Anim Nutr, 95: 40-46.
- Mahrous KF, Aboelenin MM, Rashed MA et al (2020). Detection of polymorphism within leptin gene in Egyptian river buffalo and predict its effects on different molecular levels. J Genet Eng Biotechnol Dec; 18: 6.
- McFadin EL, Morrison CD, Buff PR et al (2002). Leptin concentrations in periparturient ewes and their subsequent offspring. J Anim Sci, 80:738-743.
- Schoeller DA, Cella LK, Sinha MK, Caro JF (1997). Entrainment of the diurnal rhythm of plasma leptin to meal timing. J Clin Invest, 100: 1882–1887.
- Sedykh TA, Kalashnikova LA, Gizatullin RS et al (2020). Effects of Leptin Gene Polymorphism on Beef Cattle Performance. Russian agricultural sciences. 46 (6): 614-618.
- Tajik J and Nazifi S (2011). A study of correlation of serum leptin with trace elements in water buffalo (Bubalus bubalis). Pakistan Veterinary Journal. 31(3):231-214.
- Thomas MG, Enns RM, Hallford DM et al (2002). Relationships of metabolic hormones and serum glucose to growth and reproductive development in performance-tested Angus, Brangus and Brahman bulls. J Anim Sci, 80:757-767.
- Topal CA (2004). Serum leptin levels in patients with breast cancer and their relationship with histological parameters. Master thesis. Ministry of Health Dr. Lütfi Kırdar Kartal Training and Research Hospital, Department of Internal Medicine, Istanbul.
- Yonekura S, Kitade K, Furukawa G et al (2002). Effects of aging and weaning on mRNA expression of leptin and CCK receptors in the calf rumen and abomasum. Domest Anim Endocrinol 22: 25–35.
- Zieba DA, Amstalden M, Morton S et al (2004). Regulatory roles of leptin at the hypothalamic-hypopyseal axis before and after sexual maturation in catlle. Biol Reprod 71:804-812.
There are 27 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Clinical Sciences, Health Care Administration, Veterinary Surgery |
| Journal Section | Research Article |
| Authors | |
| Early Pub Date | December 22, 2022 |
| Publication Date | December 30, 2022 |
| Published in Issue | Year 2022Volume: 7 Issue: 3 |
